Dr Sub Reddy
Senior Lecturer in Applied Analytical Chemistry
Qualifications: BSc PhD CChem MRSC
Email: s.reddy@surrey.ac.uk
Phone: Work: 01483 68 6396
Room no: 34 AZ 03
Further information
Biography
Dr. Sub Reddy (C.Chem. MRSC) obtained his first class degree in Chemistry from the University of Manchester. He received his Ph.D. in Membrane-based Electrochemical Biosensing from the same University (1996). His post-doctoral research interests have included the development of quartz crystal-based biosensors, operating in the liquid phase (University of Wales, Bangor; 1994-1997) and the development of application-specific odour sensors (UMIST, Manchester; 1997-1998).
Dr. Reddy is, at present, Senior Lecturer in Applied Analytical Chemistry in the Faculty of Health and Medical Sciences at the University of Surrey. Current research interests include the development of smart, permselective and biocompatible molecular imprinted polymers and membrane materials for the sensor/sample interface and the advancement of smart materials-based electrochemical, quartz crystal and optical sensors for medical, food and environmental applications. He is particularly interested in developing hydrogel-based molecularly imprinted polymers (HydroMIPs) for the determination of protein markers and other biomarkers and construction of biosensors
Research Interests
Materials and Biosensor Research:
- Biomolecularly Imprinted Polymers (Bionanotechnology)
- Smart Materials (Nanotechnology)
- Electrochemical Biosensors
- Quartz Crystal Microbalance Biosensors
- Biocompatibility
Research Collaborations
University of Surrey:
FHMS
Dr D Stevenson (joint Ph.D student; 3 papers published)
FEPS
Dr Stephen Sweeney (joint Ph.D. student; EPSRC grant submitted)
Dr Karen Kirkby (EPSRC network grant)
Dr Mike Hughes (joint paper published;)
International
Dr Shigeru Kurosawa, Japan (Daiwa funding and papers published);
Professor Andreas Holzenburg Texas A&M University, USA (DTI funding; paper)
Professor Gerry Meininger, University of Missouri-Columbia, USA (DTI funding; paper)
Professor Marcel Gindre, University of Cergy-Pontoise, France (Visiting Associate Professor)
Dr Keith Warriner, University of Guelph, ON, Canada (NCFPD funding)
Publications
Journal articles
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(2012) 'Protein crystallization and biosensor applications of hydrogel-based molecularly imprinted polymers.'. Biomacromolecules, United States: 13 (12), pp. 3959-3965.doi: 10.1021/bm301189f
- . (2011) 'Investigation of polyviologens as oxygen indicators in food packaging (vol 152, pg 63, 2011)'. ELSEVIER SCIENCE SA SENSORS AND ACTUATORS B-CHEMICAL, 159 (1), pp. 342-344.
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(2011) 'Electrochemical probing of selective haemoglobin binding in hydrogel-based molecularly imprinted polymers'. Elsevier Electrochimica Acta, 56 (25), pp. 9203-9208.Full text is available at: http://epubs.surrey.ac.uk/7316/
Abstract
An electrochemical method has been developed for the probing of hydrogel-based molecularly imprinted polymers (HydroMIPs) on the surface of a glassy carbon electrode. HydroMIPs designed for bovine haemoglobin selectivity were electrochemically characterised and their rebinding properties were monitored using cyclic voltammetry. The electrochemical reduction of bovine oxyhaemoglobin (BHb) in solution was observed to occur at −0.460 V vs (Ag/AgCl) in 150 mM phosphate buffer solution (PBS). When the protein was selectively bound to the MIP, the electrochemical reduction of oxyhaemoglobin could be observed at a similar peak potential of −0.480 V vs (Ag/AgCl). When analysing the non-imprinted control polymer (NIP) interfaced at the electrode, which contained no protein, the peak reduction potential corresponded to that observed for dissolved oxygen in solution (−0.65 V vs (Ag/AgCl)). MIP and NIP (in the absence of protein) were interfaced at the electrode and protein allowed to diffuse through the polymers from the bulk solution end to the electrode. It was observed that whereas NIP exhibited a protein response within 10 min of protein exposure, up to 45 min of exposure time was required in the case of the MIP before a protein response could be obtained. Our results suggest that due to the selective nature of the MIP, BHb arrival at the electrode via diffusion is delayed by the MIP due to attractive selective interactions with exposed cavities, but not the NIP which is devoid of selective cavities.
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(2011) 'Protein crystallization facilitated by molecularly imprinted polymers'. NATIONAL ACADEMY OF SCIENCES Proceedings of the National Academy of Sciences USA, 108 (27), pp. 11081-11086.Full text is available at: http://epubs.surrey.ac.uk/7322/
Abstract
We present a new initiative and its application, namely the design of molecularly imprinted polymers (MIPs) for producing protein crystals which are essential for determining high-resolution 3-D structures of proteins. MIPs, also referred to as ‘smart materials’ are made to contain cavities capable of rebinding protein, thus the fingerprint of the protein created on the polymer allows it to serve as an ideal template for crystal formation. We have shown that six different MIPs induced crystallization of nine proteins, yielding crystals in conditions that do not give crystals otherwise. The incorporation of MIPs in screening experiments gave rise to crystalline hits in 8- 10% of the trials for three target proteins. These hits would have been missed using other known nucleants. MIPs also facilitated the formation of large single crystals at metastable conditions for seven proteins. Moreover, the presence of MIPs has led to faster formation of crystals in all cases where crystals would appear eventually and to major improvement in diffraction in some cases. The MIPs were effective for their cognate proteins and also for other proteins, with size-compatibility being a likely criterion for efficacy. Atomic Force Microscopy (AFM) measurements demonstrated specific affinity between the MIPs cavities and a protein-functionalised AFM tip, corroborating our hypothesis that due to the recognition of proteins by the cavities, MIPs can act as nucleation inducing substrates (nucleants) by harnessing the proteins themselves as templates.
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(2011) 'Investigation of polyviologens as oxygen indicators in food packaging'. ELSEVIER SCIENCE SA SENSOR ACTUAT B-CHEM, 152 (1), pp. 63-67.Full text is available at: http://epubs.surrey.ac.uk/29728/
Abstract
A triggered oxygen indicator, formulated from a combination of electrochrome, titanium dioxide and EDTA, was evaluated for use in modified atmosphere packaging. Methylene blue was not an ideal electrochrome due to its slow reduction to the leuco form and fast subsequent oxidation by oxygen present at low concentrations, >0.1%. Polyviologen electrochromes showed much faster reduction after exposure to UV light. Thionine and 2,2′-dicyano-1,1′-dimethylviologen dimesylate, which have more anodic reduction potentials compared to methylene blue, can be used to produce oxygen indicators with decreased sensitivity to oxygen. These indicators can be used to detect oxygen even when levels increase up to 4.0%.
- . (2011) 'Erratum to "Investigation of polyviologens as oxygen indicators in food packaging" [Sensors Actuat. B 152 (2011) 63-67] (DOI:10.1016/j.snb.2010.09.047)'. Sensors and Actuators, B: Chemical,
- . (2010) 'Synthesis and Characterization of Organic Thin Film Using Atmospheric-Pressure Plasma Polymerization'. MYU SENSORS AND MATERIALS, 22 (7), pp. 337-345.
- . (2009) 'Gas Sorption of Acetone, Diethyl Ether, Toluene, Acetic Acid, and Ammonia on Plasma-Polymerized Hexamethyldisiloxane Films Coated with Quartz Crystal Microbalance'. TECHNICAL ASSOC PHOTOPOLYMERS,JAPAN J PHOTOPOLYM SCI TEC, 22 (6), pp. 743-745.
- . (2009) 'The development of a compact free spectral range semiconductor laser biosensor'. EUROPEAN OPTICAL SOC JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS, 4 Article number ARTN 09013
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(2007) 'Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs)'. SPRINGER JOURNAL OF MATERIALS SCIENCE, 42 (22), pp. 9465-9468.Full text is available at: http://epubs.surrey.ac.uk/205740/
- . (2007) 'An integrated dielectrophoretic quartz crystal microbalance (DEP-QCM) device for rapid biosensing applications'. ELSEVIER ADVANCED TECHNOLOGY BIOSENSORS & BIOELECTRONICS, 23 (2), pp. 225-232.
- . (2007) 'Surface plasmon resonance-based trace detection of small molecules by competitive and signal enhancement immunoreaction'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, 591 (2), pp. 191-194.
- . (2007) 'On-demand fabrication of microplasma-polymerized styrene films using automatic motion controller'. TECHNICAL ASSOC PHOTOPOLYMERS,JAPAN JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 20 (2), pp. 215-220.
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(2006) 'Quantification and confocal imaging of protein specific molecularly imprinted polymers'. AMER CHEMICAL SOC BIOMACROMOLECULES, 7 (9), pp. 2560-2564.doi: 10.1021/bm060494dFull text is available at: http://epubs.surrey.ac.uk/205734/
- . (2004) 'Amperometric sensor study of the selectivity of poly(vinyl chloride) membranes plasticised with surfactants and liquid crystals'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, 508 (1), pp. 71-78.
- . (2002) 'Quartz crystal microbalance determination of organophosphorus and carbamate pesticides'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, 469 (2) Article number PII S0003-2670(02)00668-2 , pp. 189-196.
- . (2002) 'Optimisation of the enzyme-based determination of hydrogen peroxide using the quartz crystal microbalance'. ELSEVIER ADVANCED TECHNOLOGY BIOSENSORS & BIOELECTRONICS, 17 (9) Article number PII S0956-5663(02)00057-X , pp. 735-739.
- . (2002) 'Entrapment of glucose oxidase in non-porous poly(vinyl chloride)'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, 461 (1) Article number PII S0003-2670(02)00246-5 , pp. 57-64.
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(2002) 'Determination of 4-aminophenol using the quartz crystal microbalance sensor'. ROYAL SOC CHEMISTRY ANALYST, 127 (3), pp. 368-372.doi: 10.1039/b111301c
- . (1999) 'PVC as a sensor membrane material: influence of solvent casting variables'. KLUWER ACADEMIC PUBL JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 10 (5), pp. 295-300.
Conference papers
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(2007) 'Optimisation of distributed feedback laser biosensors'. INST ENGINEERING TECHNOLOGY-IET IET OPTOELECTRONICS, Cardiff, WALES: 21st Conference on Semiconductor Integrated Optoelectronics (SIOE) 1 (6), pp. 266-271.Full text is available at: http://epubs.surrey.ac.uk/733533/
- . (2005) 'Electrochemical disinfection, an environmentally acceptable method of drinking water disinfection?'. PERGAMON-ELSEVIER SCIENCE LTD ELECTROCHIMICA ACTA, Thessaloniki, GREECE: 55th Annual Meeting of the International-Society-of-Electrochemistry 50 (25-26), pp. 5270-5277.
- . (2005) 'Investigation of protein imprinting in hydrogel-based molecularly imprinted polymers (HydroMIPs)'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, Cardiff, WALES: 3rd International Workshop on Molecularly Imprinted Polmers 542 (1), pp. 61-65.
- . (2003) 'Enzyme-based determination of cholesterol using the quartz crystal acoustic wave sensor'. ELSEVIER SCIENCE BV ANALYTICA CHIMICA ACTA, CORNELL UNIV ITHACA, ITHACA, NEW YORK: 5th Workshop on Biosensors and Biological Techniques in Environmental Analysis 487 (1), pp. 91-100.
- . (2000) 'Detection of phenol in aqueous solution using an AT-cut quartz thickness shear mode sensor'. ELSEVIER SCIENCE BV MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, NATL INST ADV INTERDISCIPLINARY RES, TSUKUBA, JAPAN: Biomolecular Mechanism and Design Workshop 99: an Approach to Environmental Sensing 12 (1-2), pp. 23-28.
Book chapters
- . (2008) 'Principles of bacterial detection'. in Zourob M, Elwary S, Turner A (eds.) Principles of bacterial detection Springer Verlag Article number 29 , pp. 785-814.
Teaching
Physical and Bioinorganic Chemistry
Practical Biochemistry and Chemistry
Key Skills I
Chemical and Biochemical Foundations
Food Analysis and Quality Control
Analytical Biochemistry
Applied Organic Chemistry
Biochemical Techniques
Analytical Techniques 1
Analytical Techniques 2
Analytical Techniques 3
Physical Chemistry and Green Frontiers
Bionanotechnology
Advanced Bionanotechnology
MSc Clinical Biochemistry
MSc Toxicology
Departmental Duties
Senior Lecturer in Applied Analytical Chemistry
Admissions Tutor (Chemical Sciences)
Theme Leader (Materials and Nanobiology Theme)
Affiliations
Member of Royal Society of Chemistry (CChem MRSC)
