Dr Alexis Bailey

Lecturer in Neuropharmacology

Qualifications: BSc, PhD

Email:
Phone: Work: 01483 68 2564

Office hours

04AY02

9-5 with appointment

Further information

Biography

Lecturer in Neuropharmacology (from 2010)
RCUK Academic Fellow in In Vivo Toxicology and Safety Pharmacology, University of Surrey (2008-2010)
Postdoctoral Research Fellow, FP6 EU grant (GENADDICT), University of Surrey (2005-2008)
Postdoctoral Research Fellow, The Laboratory of the Biology of Addictive Diseases, Rockefeller University, New York (2003-2005)
PhD in Neuropharmacology, University of Surrey (1999-2002)
Research Assistant, Bayer Pharmaceutical Research Division, Stoke Poges (1997-1998)
BSc Biochemistry (Medical), University of Surrey (1995-1999)

Research Interests

Dr Bailey’s current research projects are listed below. He is actively looking for prospective PhD students interested in these project areas. Students that are on line for a first or upper second class honours degree in one of the biomedical sciences, or equivalent, should send their cv by e-mail to a.bailey@surrey.ac.uk

Research Group
Sleep, Chronobiology and Addiction

Drug Addiction and Neuropsychiatric Disorders

Drug addiction is serious chronic relapsing brain disorder with severe health and socioeconomic consequences. It’s the fourth most costly mental disorder in Europe with 40-50% comorbidity with major depression and anxiety. Nonetheless, stigmatization has been the prevailing response of society which results in social isolation of drug addicts. Our laboratory's main areas of interest are geared towards better understanding the genetic/molecular and psychobiological mechanisms underpinning addiction and the affective and social consequences of this disorder. Understanding these mechanisms will help us develop better therapeutic tools for addictive disorders and psychiatric comorbidities that amount to billions of pounds per year in health costs and lost productivity in the UK. We have made significant contribution in identifying the role of specific components of neurotransmitter systems including the endogenous opioid, nicotinic, adenosine, glutamatergic, dopaminergic and NADPH oxidase

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Endogenous opioid neurocircuitrieshypothesized to be recruited during the transition from positive to negative reinforcement in cocaine addiction. For more detail see our review published in British Journal Pharmacology

 In particular, we have recently identified a specific role for the “social neuropeptide”, the “love hormone” oxytocin in the long-term effects of opioid use and have demonstrated the oxytocin system as a target for the development of novel treatments for opioid withdrawal-related emotional impairment and relapse prevention.  Current research is utilizing behavioural pharmacology techniques including conditioned place preference, reinstatement models freely moving in vivo microdialysis,  as well as molecular and imaging techniques in order to tackle the research questions posed. We also have an ongoing collaboration with the department of Psychology (Cropley), University of Surrey trying to delineate the mechanism by which exercise may reduce craving to nicotine with a combination of human and in vivo studies.

Dysregulation of the endogenous oxytocinergic system is likely to be driving the emotional impairment that abstinent opioid abusers experience. For more detail see our recent paper published in Neuropsychopharmacology

 

Microbiome-Gut-Brain Axis Regulation

There has been a big rise of the gut microbiota as a major topic of research in biology. Studies are revealing how variations and changes in the composition of the gut microbiota influence physiology and contributes to diseases. New evidence shows that gut microbiota also communicates with the CNS and thereby influences brain function and behavior. We are currently investigating the effect of early life environmental influences, such as weaning and stress, on gut-brain axis which would ultimately affect brain function and behaviour.

Gut-brain axis communication

 

Awards and Prizes

Aptuit prize from the British Pharmacological Society based on published outstanding work in Integrative Pharmacology (2010)

Shortlisted for the Trends in Pharmacological Sciences Young Pharmacologist of the Year Competition (2006, 2008)                                                                                                                                                                                    

IUPHAR Early Career Investigator Award (2006)

The College of the Problems of Drug Dependence Early Career Investigator Award (2005)

RESEARCH FUNDING

Bailey A (PI), Swann J, Kitchen I
BBSRC-University of Surrey Doctoral Training in Food security (2013-2017)  
£ 80,000
Impact of prolonged maternal milk exposure on gut microbiota, brain development and behaviour

Bailey A (PI)
BBSRC In Vivo Strategic Skill Awards (2013-2017)
£20,000
Impact of prolonged maternal milk exposure on gut microbiota, brain development and behaviour

Bailey A (PI)
Royal Society project grant (2013-2014)
£14,500
Oxytocin analogues: A novel target for preventing relapse in opioid addiction?

Bailey A (PI), Camarini R
FAPESP (Brazil)-University of Surrey Research Grant (2012-2013)
£10,000
Effect and neurobiological mechanisms of environmental manipulations on ethanol and nicotine induced behavioural effects: involvement of the stress circuitry

Bailey A (PI)
Santander mobilization grant for collaborative project with University Sao Paulo, Brazil (2011, 2013)
£5,000

Bailey A (Co.I), Kitchen I (PI), Cropley M, Chen Y, 
MRC/ESRC Interdisciplinary Research Studentship (2009-2013)
£82,000
Effect and neurobiological mechanisms of exercise on nicotine craving

PhD Students 

2013-2017 Aya Osman, BBSRC Doctoral Training in Food Security (P.I. Bailey)
Impact of prolonged maternal milk exposure on gut microbiota, brain
development and behaviour

2013-2016 Loreto Rojo Gonzalez, Self-Funded (Co.I. Bailey)
Role of GABAb receptors in addictive processes

2011-2014 Polymnia Georgiou, Self-Funded (P.I. Bailey)
Neurobiological mechanisms of drug relapse

2010-2013 Pamela Farshim, University of Surrey Faculty Studentship: (Co.I. Bailey)
Impact of prolonged maternal milk exposure on gut microbiota, brain
development and behaviour

2009-2013 Helen Keyworth, MRC/ESRC Interdisciplinary Studentship (Co.I. Bailey)
Effect and neurobiological mechanisms of exercise on nicotine craving

2010-2013 Panos Zanos, Self-Funded (P.I. Bailey)
The role of oxytocin in drug addiction

2009-2012 Sherie R Wright, University of Surrey Faculty Studentship (P.I. Bailey)
The role of A2A receptors in drug addiction: Interaction with mGluR5

Visiting Researchers

2013-2014 Vicky Wright, Pharmacology dept, University of Bath, UK

2013-2014 Victoria Sebok, Chemistry dept, University of Surrey, UK

2013 Juan-Antonio Garcia Carmona, Pharmacology dept, University of Murcia, Spain

2013 Mariana Rae, Pharmacology dept, University Sao Paulo, Brazil

2012 Elisabet Freixas, Pharmacology dept, Pompeu Fabra University, Spain

Research Collaborations

University of Surrey
Prof. Ian Kitchen, Professor of Neuropharmacology, Dept. Biochemistry & Physiology
Prof. Susanna Hourani, Professor of Pharmacology, Dept. Biochemistry & Physiology
Prof. Jian Mei Li, Professor of Cardiovascular Biology, Dept. Biochemistry & Physiology
Prof. Mark Cropley, Professor of Health Psychology, Dept. Psychology
Dr. Raphaelle Winsky-Sommerer, Senior Lecturer in Sleep & Circadian Rhythms
Dr. Ying Chen, Lecturer in Neurophysiology, Dept. Biochemistry & Physiology
Dr. Daan van der Veen, Lecturer in Sleep & Chronobiology, Dept. Biochemistry & Physiology
Dr. Simon Archer, Reader in Chronobiology, Dept. Biochemistry & Physiology
Dr Shahida Shafi, Research Fellow, Dept. Biochemistry & Physiology
Dr. Priti Chivers, Research Fellow, Dept. Biochemistry & Physiology
Dr. Kevin Well, Senior Lecturer in Medical Imaging, Dept. Electronic Engineering

External Collaborations
Prof. Rafael Maldonado, Pompeu Fabra University, Barcelona
Prof. Brigitte Kieffer, Institut de Genetique et de Biologie Moleculaire et Celulaire, Director at INSERM
Prof. Andreas Zimmer, Director of Institute for Molecular Psychiatry, University of Bonn
Prof. Rosana Camarini, Department of Pharmacology, University Sao Paolo, Brazil
Dr. MJ Kreek, Labaratory of the Biology of Addictive Diseases, Rockefeller University, New York
Prof. Catherine Ledent, Director of IRIBHM, University of Brussels
Dr. Heidi Lesscher, Faculty of Veterinary Medicine, University of Utrecht
Dr. Leda Kovatsi, School of Medicine, Aristotle University of Thessaloniki
Dr. Jonathan Swann, Department of Food and Nutritional Sciences, University of Reading
Dr. Lisa Wells, PET Biologist, Imanova, Centre for Imaging Sciences
Dr. Christopher Bailey, Department of Pharmacy & Pharmacology, University of Bath
Prof. Sue Wonnacott, Department of Biology & Biochemistry, University of Bath
Dr. Fiona Robinson, Surrey & Borders Partnership NHS Foundation Trust, Chertsey

Publications

Journal articles

  • Chao MR, Fragou D, Zanos P, Hu CW, Bailey A, Kouidou S, Kovatsi L. (2014) 'Epigenetically modified nucleotides in chronic heroin and cocaine treated mice.'. Toxicol Lett, Netherlands: 229 (3), pp. 451-457.

    Abstract

    Epigenetic changes include the addition of a methyl group to the 5' carbon of the cytosine ring, known as DNA methylation, which results in the generation of the fifth DNA base, namely 5-methylcytosine. During active or passive demethylation, an intermediate modified base is formed, 5-hydroxymethylcytosine. We have currently quantified 5-methylcytosine and 5-hydroxymethylcytosine in the liver and brain of mice treated with cocaine or heroin, using liquid chromatography/tandem mass spectrometry (LC-MS/MS). Our results show that global 5-methylcytosine levels are not affected by heroin or cocaine administration, neither in the liver nor in the brain. However, 5-hydroxymethylcytosine levels are reduced in the liver following cocaine administration, while they are not affected by cocaine in the brain or by heroin administration in the liver and the brain. Elucidation of the epigenetic phenomena that takes place with respect to drug abuse and addiction, via quantitative analysis of different modified bases, may enable a better understanding of the underlying mechanisms and may lead to more personalized and effective treatment options.

  • Zanos P, Wright SR, Georgiou P, Yoo JH, Hourani SM, Kitchen I, Winsky-Sommerer R, Bailey A, Ledent C. (2014) 'Chronic methamphetamine treatment induces oxytocin receptor up-regulation in the amygdala and hypothalamus via an adenosine A receptor-independent mechanism'. Pharmacology Biochemistry and Behavior, 119, pp. 72-79.

    Abstract

    There is mounting evidence that the neuropeptide oxytocin is a possible candidate for the treatment of drug addiction. Oxytocin was shown to reduce methamphetamine self-administration, conditioned place-preference, hyperactivity and reinstatement in rodents, highlighting its potential for the management of methamphetamine addiction. Thus, we hypothesised that the central endogenous oxytocinergic system is dysregulated following chronic methamphetamine administration. We tested this hypothesis by examining the effect of chronic methamphetamine administration on oxytocin receptor density in mice brains with the use of quantitative receptor autoradiographic binding. Saline (4 ml/kg/day, i.p.) or methamphetamine (1 mg/kg/day, i.p.) was administered daily for 10 days to male, CD1 mice. Quantitative autoradiographic mapping of oxytocin receptors was carried out with the use of [I]-vasotocin in brain sections of these animals. Chronic methamphetamine administration induced a region specific upregulation of oxytocin receptor density in the amygdala and hypothalamus, but not in the nucleus accumbens and caudate putamen. As there is evidence suggesting an involvement of central adenosine A receptors on central endogenous oxytocinergic function, we investigated whether these methamphetamine-induced oxytocinergic neuroadaptations are mediated via an A receptor-dependent mechanism. To test this hypothesis, autoradiographic oxytocin receptor binding was carried out in brain sections of male CD1 mice lacking A receptors which were chronically treated with methamphetamine (1 mg/kg/day, i.p. for 10 days) or saline. Similar to wild-type animals, chronic methamphetamine administration induced a region-specific upregulation of oxytocin receptor binding in the amygdala and hypothalamus of A receptor knockout mice and no genotype effect was observed. These results indicate that chronic methamphetamine use can induce profound neuroadaptations of the oxytocinergic receptor system in brain regions associated with stress, emotionality and social bonding and that these neuroadaptations are independent on the presence of A receptors. These results may at least partly explain some of the behavioural consequences of chronic methamphetamine use. © 2013 Elsevier Inc. All rights reserved.

  • Yoo JH, Bailey A, Borsodi A, Tóth G, Matifas A, Kieffer BL, Kitchen I. (2014) 'Knockout subtraction autoradiography: a novel ex vivo method to detect heteromers finds sparse KOP receptor/DOP receptor heterodimerization in the brain.'. Eur J Pharmacol, Netherlands: 731, pp. 1-7.

    Abstract

    Several methodological approaches suggest that receptor heteromers exist in cell systems, but their presence in physiological tissue is widely contentious. We describe a novel method to determine if heterodimers exist in brain tissue sections using autoradiographic binding comparisons from single and double gene knockout mice, where tissues either have a full receptor complement and can form heterodimers, or are incapable of making heterodimers. We have tested this model, which we have named Knockout Subtraction Autoradiography, to determine if heterodimerisation of the kappa (KOP) and delta opioid (DOP) receptors occurs, as evidence from binding studies in cell systems suggest they are present in the brain. Using labeling of putative KOP receptor/DOP receptor heterodimers with either [(3)H]bremazocine or with [(3)H]naltrindole, two ligands which were used to provide evidence suggesting that these opioid receptor subtypes heterodimerize, we have applied a subtraction equation model based on the principle that receptor gene double knockout of either MOP receptor/KOP receptor (DOP receptor expression only) or MOP receptor/DOP receptor (KOP receptor expression only) produces tissue incapable of making the KOP receptor/DOP receptor heterodimer. We have shown in most brain regions that the labeling fits a simple additive model of monomer labeling, but that in a few brain regions opioid receptor heterodimerization does occur. The data does not support the conclusion that KOP receptor/DOP receptor heterodimerisation is widespread in the central nervous system, but does indicate that this novel methodology can detect heterodimerisation, when ligands with distinct binding affinities for monomer and heterodimer forms exist.

  • Zanos P, Georgiou P, Wright SR, Hourani SM, Kitchen I, Winsky-Sommerer R, Bailey A. (2014) 'The oxytocin analogue carbetocin prevents emotional impairment and stress-induced reinstatement of opioid-seeking in morphine-abstinent mice'. Neuropsychopharmacology, 39 (4), pp. 855-865.

    Abstract

    The main challenge in treating opioid addicts is to maintain abstinence due to the affective consequences associated with withdrawal which may trigger relapse. Emerging evidence suggests a role of the neurohypophysial peptide oxytocin (OT) in the modulation of mood disorders as well as drug addiction. However, its involvement in the emotional consequences of drug abstinence remains unclear. We investigated the effect of 7-day opioid abstinence on the oxytocinergic system and assessed the effect of the OT analogue carbetocin (CBT) on the emotional consequences of opioid abstinence, as well as relapse. Male C57BL/6J mice were treated with a chronic escalating-dose morphine regimen (20-100 mg/kg/day, i.p.). Seven days withdrawal from this administration paradigm induced a decrease of hypothalamic OT levels and a concomitant increase of oxytocin receptor (OTR) binding in the lateral septum and amygdala. Although no physical withdrawal symptoms or alterations in the plasma corticosterone levels were observed after 7 days of abstinence, mice exhibited increased anxiety-like and depressive-like behaviors and impaired sociability. CBT (6.4 mg/kg, i.p.) attenuated the observed negative emotional consequences of opioid withdrawal. Furthermore, in the conditioned place preference paradigm with 10 mg/kg morphine conditioning, CBT (6.4 mg/kg, i.p.) was able to prevent the stress-induced reinstatement to morphine-seeking following extinction. Overall, our results suggest that alterations of the oxytocinergic system contribute to the mechanisms underlying anxiety, depression, and social deficits observed during opioid abstinence. This study also highlights the oxytocinergic system as a target for developing pharmacotherapy for the treatment of emotional impairment associated with abstinence and thereby prevention of relapse.

  • Hambsch B, Otte DM, Racz I, Zimmer A, Keyworth H, Lind J, Kitchen I, Bailey A. (2014) 'Chronic nicotine improves short-term memory selectively in a G72 mouse model of schizophrenia'. British Journal of Pharmacology, 171 (7), pp. 1758-1771.

    Abstract

    Background and Purpose The prevalence of smoking in schizophrenia patients is exceptionally high; it is not known why but many researchers suggest that smoking constitutes a form of self-medication. Among the symptoms of schizophrenia that may be improved by nicotine are cognitive deficits. Hence, we studied the effects of long-term nicotine administration on cognition in a genetic animal model of schizophrenia susceptibility, G72-transgenic (G72Tg) mice. Experimental Approach The effect of long-term nicotine or saline, administered by osmotic minipumps, on different cognitive domains was assessed in G72Tg mice and controls using a battery of behavioural tests. To investigate the mechanism underlying phenotypic differences, quantitative autoradiographic mapping of nACh receptor subtypes was performed in forebrain structures to explore effects of chronic nicotine exposure on nACh receptor density in wild-type (WT) and G72Tg mice. Key Results Genotype significantly affected the cognitive effects of chronic nicotine administration. Whereas chronic nicotine disrupted cognitive performance in WT mice, it was effective at restoring impaired prepulse inhibition, working memory and social recognition in G72Tg mice. However, long-term spatial learning was further impaired by nicotine in transgenic animals. In contrast, associative learning was protected by G72-expression against the adverse nicotine effects seen in WT animals. G72-expression did not decisively influence nicotine-induced up-regulation of the α4β2subtype, whereas α7nACh receptor density was differentially altered by genotype or by a genotype·treatment interaction in specific brain areas, most notably hippocampal subregions. Conclusions and Implications Our data support the hypothesis that nicotine self-medication of schizophrenics improves cognitive symptoms, possibly by facilitating nicotine-induced α7nACh receptor activation in the hippocampus. © 2014 The British Pharmacological Society.

  • Valzachi MC, Camarini R, Teodorov E, Marcourakis T, Bailey A. (2013) 'Enhancement of Behavioral Sensitization, Anxiety-Like Behavior, and Hippocampal and Frontal Cortical CREB Levels Following Cocaine Abstinence in Mice Exposed to Cocaine during Adolescence'. PLoS ONE, 8 (10)

    Abstract

    Adolescence has been linked to greater risk-taking and novelty-seeking behavior and a higher prevalence of drug abuse and risk of relapse. Decreases in cyclic adenosine monophosphate response element binding protein (CREB) and phosphorylated CREB (pCREB) have been reported after repeated cocaine administration in animal models. We compared the behavioral effects of cocaine and abstinence in adolescent and adult mice and investigated possible age-related differences in CREB and pCREB levels. Adolescent and adult male Swiss mice received one daily injection of saline or cocaine (10 mg/kg, i.p.) for 8 days. On day 9, the mice received a saline injection to evaluate possible environmental conditioning. After 9 days of withdrawal, the mice were tested in the elevated plus maze to evaluate anxiety-like behavior. Twelve days after the last saline/cocaine injection, the mice received a challenge injection of either cocaine or saline, and locomotor activity was assessed. One hour after the last injection, the brains were extracted, and CREB and pCREB levels were evaluated using Western blot in the prefrontal cortex (PFC) and hippocampus. The cocaine-pretreated mice during adolescence exhibited a greater magnitude of the expression of behavioral sensitization and greater cocaine withdrawal-induced anxiety-like behavior compared with the control group. Significant increases in CREB levels in the PFC and hippocampus and pCREB in the hippocampus were observed in cocaine-abstinent animals compared with the animals treated with cocaine in adulthood. Interestingly, significant negative correlations were observed between cocaine sensitization and CREB levels in both regions. These results suggest that the behavioral and neurochemical consequences of psychoactive substances in a still-developing nervous system can be more severe than in an already mature nervous system. © 2013 Valzachi et al.

  • Metaxas A, Al-Hasani R, Farshim P, Tubby K, Berwick A, Ledent C, Hourani S, Kitchen I, Bailey A. (2013) 'Genetic deletion of the adenosine A2A receptor prevents nicotine-induced upregulation of α7, but not α4β2* nicotinic acetylcholine receptor binding in the brain.'. Neuropharmacology, 71, pp. 228-236.

    Abstract

    Considerable evidence indicates that adenosine A2A receptors (A2ARs) modulate cholinergic neurotransmission, nicotinic acetylcholine receptor (nAChR) function, and nicotine-induced behavioural effects. To explore the interaction between A2A and nAChRs, we examined if the complete genetic deletion of adenosine A2ARs in mice induces compensatory alterations in the binding of different nAChR subtypes, and whether the long-term effects of nicotine on nAChR regulation are altered in the absence of the A2AR gene. Quantitative autoradiography was used to measure cytisine-sensitive [(125)I]epibatidine and [(125)I]α-bungarotoxin binding to α4β2* and α7 nAChRs, respectively, in brain sections of drug-naïve (n = 6) or nicotine treated (n = 5-7), wild-type and adenosine A2AR knockout mice. Saline or nicotine (7.8 mg/kg/day; free-base weight) were administered to male CD1 mice via subcutaneous osmotic minipumps for a period of 14 days. Blood plasma levels of nicotine and cotinine were measured at the end of treatment. There were no compensatory developmental alterations in nAChR subtype distribution or density in drug-naïve A2AR knockout mice. In nicotine treated wild-type mice, both α4β2* and α7 nAChR binding sites were increased compared with saline treated controls. The genetic ablation of adenosine A2ARs prevented nicotine-induced upregulation of α7 nAChRs, without affecting α4β2* receptor upregulation. This selective effect was observed at plasma levels of nicotine that were within the range reported for smokers (10-50 ng ml(-1)). Our data highlight the involvement of adenosine A2ARs in the mechanisms of nicotine-induced α7 nAChR upregulation, and identify A2ARs as novel pharmacological targets for modulating the long-term effects of nicotine on α7 receptors.

  • Fragou D, Njau S, Kovatsi L, Zanos P, Kitchen I, Bailey A, Kouidou S. (2013) 'Effect of chronic heroin and cocaine administration on global DNA methylation in brain and liver'. Toxicology Letters, 218 (3), pp. 260-265.

    Abstract

    Drug abuse is associated with epigenetic changes, such as histone modifications and DNA methylation. The purpose of the present study was to examine the effect of chronic cocaine and heroin administration on global DNA methylation in brain and liver. Male, 8 week old, C57BL/6. J mice received heroin in a chronic 'intermittent' escalating dose paradigm, or cocaine in a chronic escalating dose 'binge' paradigm, which mimic the human pattern of opioid or cocaine abuse respectively. Following sacrifice, livers and brains were removed and DNA was extracted from them. The extracted DNA was hydrolyzed and 2'-deoxycytidine and 5-methyl-2'-deoxycytidine were determined by HPLC-UV. The % 5-methyl-2'-deoxycytidine content of DNA was significantly higher in the brain compared to the liver.There were no differences between the control animals and the cocaine or heroin treated animals in neither of the tissues examined, which is surprising since cocaine administration induced gross morphological changes in the liver. Moreover, there was no difference in the % 5-methyl-2'-deoxycytidine content of DNA between the cocaine and the heroin treated animals. The global DNA methylation status in the brain and liver of mice chronically treated with cocaine or heroin remains unaffected, but this finding cannot exclude the existence of anatomical region or gene-specific methylation differences. This is the first time that global DNA methylation in the liver and whole brain has been studied following chronic cocaine or heroin treatment. © 2013 Elsevier Ireland Ltd.

  • Zanos P, Wright SR, Georgiou P, Yoo JH, Hourani SM, Kitchen I, Winsky-Sommerer R, Bailey A, Ledent C. (2013) 'Chronic methamphetamine treatment induces oxytocin receptor up-regulation in the amygdala and hypothalamus via an adenosine A receptor-independent mechanism'. Pharmacology Biochemistry and Behavior,

    Abstract

    There is mounting evidence that the neuropeptide oxytocin is a possible candidate for the treatment of drug addiction. Oxytocin was shown to reduce methamphetamine self-administration, conditioned place-preference, hyperactivity and reinstatement in rodents, highlighting its potential for the management of methamphetamine addiction. Thus, we hypothesised that the central endogenous oxytocinergic system is dysregulated following chronic methamphetamine administration. We tested this hypothesis by examining the effect of chronic methamphetamine administration on oxytocin receptor density in mice brains with the use of quantitative receptor autoradiographic binding. Saline (4 ml/kg/day, i.p.) or methamphetamine (1 mg/kg/day, i.p.) was administered daily for 10 days to male, CD1 mice. Quantitative autoradiographic mapping of oxytocin receptors was carried out with the use of [I]-vasotocin in brain sections of these animals. Chronic methamphetamine administration induced a region specific upregulation of oxytocin receptor density in the amygdala and hypothalamus, but not in the nucleus accumbens and caudate putamen. As there is evidence suggesting an involvement of central adenosine A receptors on central endogenous oxytocinergic function, we investigated whether these methamphetamine-induced oxytocinergic neuroadaptations are mediated via an A receptor-dependent mechanism. To test this hypothesis, autoradiographic oxytocin receptor binding was carried out in brain sections of male CD1 mice lacking A receptors which were chronically treated with methamphetamine (1 mg/kg/day, i.p. for 10 days) or saline. Similar to wild-type animals, chronic methamphetamine administration induced a region-specific upregulation of oxytocin receptor binding in the amygdala and hypothalamus of A receptor knockout mice and no genotype effect was observed. These results indicate that chronic methamphetamine use can induce profound neuroadaptations of the oxytocinergic receptor system in brain regions associated with stress, emotionality and social bonding and that these neuroadaptations are independent on the presence of A receptors. These results may at least partly explain some of the behavioural consequences of chronic methamphetamine use. © 2013 Elsevier Inc. All rights reserved.

  • Yoo JH, Kitchen I, Bailey A. (2012) 'The endogenous opioid system in cocaine addiction: what lessons have opioid peptide and receptor knockout mice taught us?'. Br J Pharmacol, England: 166 (7), pp. 1993-2014.

    Abstract

    Cocaine addiction has become a major concern in the UK as Britain tops the European 'league table' for cocaine abuse. Despite its devastating health and socio-economic consequences, no effective pharmacotherapy for treating cocaine addiction is available. Identifying neurochemical changes induced by repeated drug exposure is critical not only for understanding the transition from recreational drug use towards compulsive drug abuse but also for the development of novel targets for the treatment of the disease and especially for relapse prevention. This article focuses on the effects of chronic cocaine exposure and withdrawal on each of the endogenous opioid peptides and receptors in rodent models. In addition, we review the studies that utilized opioid peptide or receptor knockout mice in order to identify and/or clarify the role of different components of the opioid system in cocaine-addictive behaviours and in cocaine-induced alterations of brain neurochemistry. The review of these studies indicates a region-specific activation of the µ-opioid receptor system following chronic cocaine exposure, which may contribute towards the rewarding effect of the drug and possibly towards cocaine craving during withdrawal followed by relapse. Cocaine also causes a region-specific activation of the κ-opioid receptor/dynorphin system, which may antagonize the rewarding effect of the drug, and at the same time, contribute to the stress-inducing properties of the drug and the triggering of relapse. These conclusions have important implications for the development of effective pharmacotherapy for the treatment of cocaine addiction and the prevention of relapse.

  • Metaxas A, Keyworth HL, Yoo JH, Chen Y, Kitchen I, Bailey A. (2012) 'The stereotypy-inducing and OCD-like effects of chronic 'binge' cocaine are modulated by distinct subtypes of nicotinic acetylcholine receptors.'. Br J Pharmacol,

    Abstract

    Background and purpose: High rates of cigarette smoking occur in cocaine dependent individuals, reflecting an involvement of nicotinic acetylcholine receptors (nAChRs) in cocaine-elicited behaviour. This study was designed to parse the contribution of different nAChR subtypes to the behavioural and neurochemical effects of chronic cocaine treatment. Experimental approach: Cocaine (15 mg/kg, i.p.) was administered to male C57BL/6J mice in a chronic 'binge' paradigm, with and without the co-administration of the α7 preferring antagonist methyllycaconitine (MLA; 5 mg/kg; i.p.) or the β2* nAChR antagonist dihydro-β-erythroidine (DHßE; 2 mg/kg. i.p.). Quantitative autoradiography was used to examine the impact of cocaine exposure on α7 and α4β2* nAChRs, and on the high-affinity choline transporter. Key results: MLA+cocaine administration induced an intense self-grooming behaviour, indicating a likely role for α7 nAChRs in modulating this anxiogenic, compulsive-like effect of cocaine. In the major island of Calleja, a key area of action for neuroleptics, MLA+cocaine reduced choline-transporter binding compared to cocaine (±DHßE) administration. DHßE treatment prevented the induction of stereotypy sensitisation to cocaine, but prolonged locomotor sensitisation, implicating heteromeric β2* nAChRs in the neuroadaptations mediating cocaine-induced behavioural sensitisation. 'Binge' cocaine treatment region-specifically increased α4β2* nAChR binding in the midbrain dopaminergic regions: ventral tegmental area and substantia nigra pars compacta. Conclusions and implications: We show a differential, subtype-selective contribution of nAChRs to the behavioural and neurochemical sequelae of chronic cocaine administration. These data support the clinical utility of targeting specific nAChR subtypes for the alleviation of cocaine-abuse symptomatology. © 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.

  • Kovatsi L, Fragou D, Samanidou V, Njau S, Kouidou S, Bailey A. (2012) 'Evaluation of 5-methyl-2′-deoxycytidine stability in hydrolyzed and nonhydrolyzed DNA by HPLC-UV'. Bioanalysis, 4 (4), pp. 367-372.
  • Esposito M, Wells K, Bailey A, Newcombe J, Anaxagoras T, Allinson NM. (2012) 'CMOS APS in pre-clinical science: Next generation disruptive technology for multi-modality imaging'. IEEE Nuclear Science Symposium Conference Record, , pp. 1910-1913.

    Abstract

    A new large area CMOS Active Pixel Sensor has been developed as single platform technology to be used across a range of ionizing and non-ionizing imaging applications in preclinical science, ranging from imaging of protein sequences to functional analysis of radio-labeled tissue sections.We present the first images of chemiluminescence detection in western blotting with a room temperature CMOS APS. Detection performance in western blotting have been compared with the gold standard detection medium, film emulsion, showing higher dynamic range and sensitivity with this new device. We also report on our first images of [125I]Epibatidine autoradiography of brain sections using a novel large area CMOS APS. © 2012 IEEE.

  • Metaxas A, Bailey A, Flavia Barbano M, Galeote L, Maldonado R, Kitchen I. (2010) 'Differential region-specific regulation of alpha 4 beta 2*nAChRs by self-administered and non-contingent nicotine in C57BL/6J mice'. WILEY-BLACKWELL PUBLISHING, INC ADDICTION BIOLOGY, 15 (4), pp. 464-479.
  • Yoo J-H, Bailey A, Ansonoff M, Pintar JE, Matifas A, Kieffer BL, Kitchen I. (2010) 'Lack of Genotype Effect on D1, D2 Receptors and Dopamine Transporter Binding in Triple MOP-, DOP-, and KOP-Opioid Receptor Knockout Mice of Three Different Genetic Backgrounds'. WILEY-LISS SYNAPSE, 64 (7), pp. 520-527.
  • Bailey A, Metaxas A, Al-Hasani R, Keyworth HL, Forster DM, Kitchen I. (2010) 'Mouse strain differences in locomotor, sensitisation and rewarding effect of heroin; association with alterations in MOP-r activation and dopamine transporter binding'. WILEY-BLACKWELL PUBLISHING, INC EUROPEAN JOURNAL OF NEUROSCIENCE, 31 (4), pp. 742-753.
  • Fan L, Sawbridge D, George V, Teng L, Bailey A, Kitchen I, Li J-M. (2009) 'Chronic Cocaine-Induced Cardiac Oxidative Stress and Mitogen-Activated Protein Kinase Activation: The Role of Nox2 Oxidase'. AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 328 (1), pp. 99-106.
  • Bailey A, Metaxas A, Yoo JH, McGee T, Kitchen I. (2008) 'Decrease of D(2) receptor binding but increase in D(2)-stimulated G-protein activation, dopamine transporter binding and behavioural sensitization in brains of mice treated with a chronic escalating dose 'binge' cocaine administration paradigm'. WILEY-BLACKWELL EUROPEAN JOURNAL OF NEUROSCIENCE, 28 (4), pp. 759-770.
  • Bailey A, Gianotti R, Ho A, Kreek MJ. (2007) 'Downregulation of kappa-opioid receptors in basolateral amygdala and septum of rats withdrawn for 14 days from an escalating dose "Binge" cocaine administration paradigm'. WILEY-LISS SYNAPSE, 61 (10), pp. 820-826.
  • Bailey A, Yoo JH, Racz I, Zimmer A, Kitchen I. (2007) 'Preprodynorphin mediates locomotion and D-2 dopamine and mu-opioid receptor changes induced by chronic 'binge' cocaine administration'. BLACKWELL PUBLISHING JOURNAL OF NEUROCHEMISTRY, 102 (6), pp. 1817-1830.
  • Cabello J, Bailey A, Kitchen I, Prydderch M, Clark A, Turchetta R, Wells K. (2007) 'Digital autoradiography using room temperature CCD and CMOS imaging technology'. IOP PUBLISHING LTD PHYSICS IN MEDICINE AND BIOLOGY, 52 (16), pp. 4993-5011.
  • Bailey A, Gianotti R, Ho A, Kreek MJ. (2005) 'Persistent Upregulation of mu-opioid, but not adenosine, receptors in brains of long-term withdrawn escalating dose "Binge" cocaine-treated rats'. WILEY-LISS SYNAPSE, 57 (3), pp. 160-166.
  • Schlussman SD, Zhou Y, Bailey A, Ho A, Kreek MJ. (2005) 'Steady-dose and escalating dose “binge” administration of cocaine alter expression of behavioral stereotypy and striatal preprodynorphin mRNA levels in rats.'. Brain Research Bulletin, 67, pp. 169-175.
  • Bailey A, Yuferov V, Bendor J, Schlussman SD, Zhou Y, Ho A, Kreek MJ. (2005) 'Immediate withdrawal from chronic “binge” cocaine administration increases Mu-opioid receptor mRNA levels in rat frontal cortex'. Molecular Brain Research, 137, pp. 258-262.
  • Kelly M, Bailey A, Ledent C, Kitchen I, Hourani S. (2004) 'Characterization of [H-3]ZM 241385 binding in wild-type and adenosine A(2A) receptor knockout mice'. ELSEVIER SCIENCE BV EUROPEAN JOURNAL OF PHARMACOLOGY, 504 (1-2), pp. 55-59.
  • Bailey A, Weber D, Zimmer A, Zimmer AM, Hourani SMO, Kitchen I. (2004) 'Quantitative autoradiography of adenosine receptors and NBTI-sensitive adenosine transporters in the brains of mice deficient in the preproenkephalin gene'. ELSEVIER SCIENCE BV BRAIN RESEARCH, 1025 (1-2), pp. 1-9.
  • Bailey A, Davis L, Lesscher HMB, Kelly MDW, Ledent C, Hourani SMO, Kitchen I. (2004) 'Enhanced morphine withdrawal and mu-opioid receptor G-protein coupling in A(2A) adenosine receptor knockout ti ice'. BLACKWELL PUBLISHING LTD JOURNAL OF NEUROCHEMISTRY, 88 (4), pp. 827-834.
  • Kokkinou E, Wells K, Petrou M, Bailey A. (2003) 'Digital autoradiography imaging using direct irradiation of a CCD between 278-309 K'. IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC IEEE TRANSACTIONS ON NUCLEAR SCIENCE, 50 (5), pp. 1702-1707.
  • Bailey A, Hawkins RM, Hourani SMO, Kitchen I. (2003) 'Quantitative autoradiography of adenosine receptors in brains of chronic naltrexone-treated mice'. NATURE PUBLISHING GROUP BRITISH JOURNAL OF PHARMACOLOGY, 139 (6), pp. 1187-1195.
  • Lesscher HMB, Bailey A, Burbach JPH, van Ree JM, Kitchen I, Gerrits MAFM. (2003) 'Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice'. BLACKWELL PUBLISHING LTD EUROPEAN JOURNAL OF NEUROSCIENCE, 17 (5), pp. 1006-1012.
  • Bailey A, Ledent C, Kelly M, Hourani SMO, Kitchen I. (2002) 'Changes in spinal delta and kappa opioid systems in mice deficient in the A(2A) receptor gene'. SOC NEUROSCIENCE JOURNAL OF NEUROSCIENCE, 22 (21), pp. 9210-9220.
  • Bailey A, Mathes H, Kieffer B, Slowe S, Hourani SMO, Kitchen I. (2002) 'Quantitative autoradiography of adenosine receptors and NBTI-sensitive adenosine transporters in the brains and spinal cords of mice deficient in the mu-opioid receptor gene'. ELSEVIER SCIENCE BV BRAIN RESEARCH, 943 (1) Article number PII S0006-8993(02)02536-2 , pp. 68-79.
  • Bailey A, Kelland EE, Thomas A, Biggs J, Crawford D, Kitchen I, Toms NJ. (2001) 'Regional mapping of low-affinity kainate receptors in mouse brain using [H-3](2S,4R)-4-methylglutamate autoradiography'. ELSEVIER SCIENCE BV EUROPEAN JOURNAL OF PHARMACOLOGY, 431 (3), pp. 305-310.

External Oral Presentations

Conference Oral and Prize Presentations from myself and my Group

10th World Congress of Neurohypophysial Hormones 2013: Bristol
Carbetocin prevents stress-induced reinstatement to morphine-seeking in mice (Georgiou, 3rd Prize) 

European Opioid Conference 2013: Guildford
Carbetocin prevents stress-induced reinstatement to morphine-seeking in mice (Georgiou, 2nd Prize) 

British Pharmacological Society Meeting 2012: London
Complete abolition of stereotypic rearing and mGluR5 up-regulation in chronically
methamphetamine but not cocaine treated A2A receptor knock-out mice (Smith)

British Pharmacological Society Meeting 2012: London
Oxytocin analogue carbetocin reverses impaired emotional-like behaviour during prolonged abstinence from chronic morphine treatment (Zanos)

Society for Nicotine and Tobacco Research Europe Meeting 2012: Helsinki
Exercise Attenuates Withdrawal Symptoms From Nicotine In Mice (Keyworth)

British Pharmacological Society Meeting 2011: London
Delayed Weaning Influences Depressive, But Not Anxiety-like Or Social Behaviour In Rats (Farshim)

British Pharmacological Society Meeting 2011: London
Persistent brain region-specific up-regulation of the vasopressin V1a receptor following chronic cocaine and morphine administration and withdrawal in mice (Bailey)                     

European Opioid Conference 2011: Krakow
Alterations of the mGluR5, vasopressin and oxytocin receptor systems by chronic opioid administration and withdrawal (Bailey, Invited symposium talk)

European Opioid Conference 2011: Krakow
Metabotropic mGluR5- And Adenosine A2A- Receptor Interactions In Opioid Addiction (Smith)

British Pharmacological Society Meeting 2010: London
Cocaine and opioid addiction and the endogenous opioid system: “A mouse genetics approach to study the neurobiology of drug addiction” (Bailey, Invited Aptuit prize talk)

British Pharmacological Society Meeting 2009: London
Withdrawal from chronic morphine, but not cocaine induce marked upregulation of mGluR5 binding in the mouse brain (Bailey, symposium talk)

British Pharmacological Society Meeting 2008: Brighton
The role of Nox2 in chronic administration-induced cardiac oxidative stress cardiotoxicity (Fan)

British Pharmacological Society Meeting 2008: Brighton
Repeated heroin administration induces locomotion, sensitisation and conditioned place preference in C57BL/6J mice but not in DBA/2J; association with alterations in MOP-r activation and dopamine transporter binding (Bailey, symposium talk)

European Opioid Conference 2008: Ferrara
Repeated heroin administration induces locomotion, sensitisation and conditioned place preference in C57BL/6J mice but not in DBA/2J; association with alterations in MOP-r activation and dopamine transporter binding (Bailey, Invited talk, Trends in Pharmacological Sciences Young Pharmacologist of the Year Competition)

37th International Narcotics Research Conference 2007: Berlin
Morphine induced reinstatement in CD1 wildtype and adenosine A2A receptor knockout mice (Al Hasani)

British Pharmacological  Society Meeting 2006 (75th anniversary Meeting): Oxford, UK
Cocaine sensitization and dopamine D2 and Mu opioid receptor changes in chronic “binge” cocaine treated preprodynorpin knockout mice (Bailey, Invited talk, Trends in Pharmacological Sciences Young Pharmacologist of the Year Competition)

European Opioid Conference 2006: Salamanca, Spain
Enhanced cocaine sensitization in mice lacking preprodynorpin gene (Bailey, symposium talk)

36th International Narcotics Research Conference 2006: Minneapolis/St Paul, USA
Enhanced cocaine sensitization in mice lacking preprodynorpin gene (Bailey, symposium talk)

European Opioid Conference 2002: Uppsala, Sweeden
Changes in spinal delta and kappa opioid systems in mice deficient in the A2A receptor gene (Bailey)

Invited lectures at institutions

Institute of Molecular Psychiatry, University of Bonn (2013): The oxytocin analogue carbetocin prevents emotional impairment and stress-induced reinstatement of opioid-seeking in morphine abstinent mice

Rudolf Magnus Institute, University Medical Centre, Utrecht (2013): Oxytocin: a novel target for the prevention of relapse to opioid addiction?

Hot Topic talk at the Festival of Research, University of Surrey (2013): Love does not always conquer all but can our body’s love drug help us beat drug addiction?

University of Sao Paulo, Department of Pharmacology, Brazil (2012): Oxytocin: a novel target for the prevention of relapse to opioid addiction?

Imperial College, Department of Medicine (2012): Identifying neurobiological alterations in drug addiction: Implications for the development of novel relapse prevention pharmacotherapy

Centre for Integrative Mammalian Physiology & Pharmacology, Imperial College (2012): The endogenous system in cocaine addiction: what lessons have the opioid peptide and receptor knockout mice taught us?

University of Sao Paulo, Department of Pharmacology, Brazil (2011): Cocaine and opioid addiction and the endogenous opioid system: “A mouse genetics approach to study the neurobiology of drug addiction”

Navarra University, Department of Food Sciences, Nutrition, Physiology and Toxicology (2011): The endogenous system in cocaine addiction: what lessons have the opioid peptide and receptor knockout mice taught us?

The Cyprus Institute of Neurology & Genetics, Nicosia (2011): The endogenous system in cocaine addiction: what lessons have the opioid peptide and receptor knockout mice taught us?

Pompeu Fabra University, Barcelona (2010): GENADDICT Progress report

Institut de Genetique et de Biologie Moleculaire et Celulaire, INSERM (2008):GENADDICT Progress report

Rockefeller University, New York (2008): Research summary 2005-2008

University of Bonn, Institute of Molecular Psychiatry (2007): A comparison of locomotor, rewarding and neurochemical effects of chronic heroin treatment on the opioid and dopaminergic systems in C57BL/6J and DBA/2J mice

Temple University, School of Medicine, Philadelphia (2004): Interactions between opioids and adenosine in the CNS


Conference Abstracts

LARNEDA-LASBRA Joint meeting 2013: Concepcion, Chile
Mariana Rae, Polymnia Georgiou, Panos Zanos, Alexis Bailey, Rosana Camarini
The effects of environmental manipulations on ethanol-related behaviors: Involvement of the stress circuitry

10th World Congress of Neurohypophysial Hormones 2013: Bristol
Polymnia Georgiou, Panos Zanos, Susanna Hourani, Ian Kitchen, Alexis
Bailey
Carbetocin prevents stress-induced reinstatement to morphine-seeking in mice

10th World Congress of Neurohypophysial Hormones 2013: Bristol
Panos Zanos, Polymnia Georgiou, Raphaelle Winsky-Sommerer, Ian Kitchen,
Alexis Bailey
The Oxytocin Analogue Carbetocin Reverses the Negative Emotional Symptoms Induced by Protracted Opioid Abstinence

European Opioid Conference 2013: Guildford
P. Zanos, P. Georgiou, R. Winsky-Sommerer, I. Kitchen, A. Bailey
Carbetocin reverses the behavioural phenotype induced by opioid abstinence

European Opioid Conference 2013: Guildford
H. Keyworth, A. Tzaikouri, J. Lind, Y. Chen, M. Cropley, I. Kitchen, A. Bailey
Exercise reduces nicotine withdrawal symptom severity: association with hippocampal a7 nAChR upregulation

European Opioid Conference 2013: Guildford
P. Georgiou, I. Kitchen, S. Hourani, M. Ehteramyan R. Maldonado, A. Bailey
Alterations of MOP and mGlur5 receptor system following cocaine self-administration, priming-, and cue-unduced reinstatement of cocaine seeking

European Opioid Conference 2013: Guildford
P. Farshim, A. Bailey, J. Swann, B. Chakrabarti, G. Walton, I. Givens, D. Saddy and
I. Kitchen
Delayed weaning increases depressive-like behaviour, alters intestinal microflora and urinary metabolites in rats

European Opioid Conference 2013: Guildford
P. Georgiou, P. Zanos, S. Hourani, I. Kitchen, A. Bailey
Carbetocin prevents stress-induced reinstatement to morphine CPP in mice

British Pharmacological Society Meeting 2012: London
SR Smith, Zanos P, Yoo JH, Wells LA, Hourani SMO, Kitchen I, Bailey A.
Complete abolition of stereotypic rearing and mGluR5 up-regulation in chronically
methamphetamine but not cocaine treated A2A receptor knock-out mice

British Pharmacological Society Meeting 2012: London
HL Keyworth, A Tzaikouri, M Cropley, Y Chen, I Kitchen, A Bailey
Exercise Differentially Affects Nicotinic Receptor Subtypes To Attenuate Nicotine Withdrawal In Mice

British Pharmacological Society Meeting 2012: London
P Zanos, P Georgiou, R Winsky-Sommerer, I Kitchen, A Bailey
Oxytocin analogue carbetocin reverses impaired emotional-like behaviour during prolonged abstinence from chronic morphine treatment

British Pharmacological Society Meeting 2012: London
P Georgiou, I Kitchen, S Hourani, R Maldonado, A Bailey
The Effect Of Cocaine Self-administration And Priming- And Cue-induced Reinstatement On mGluR5 And MOP Receptor System In Mouse Brain

Society for Nicotine and Tobacco Research Europe Meeting 2012: Helsinki
Keyworth H, Tziakouri A, Cropley M, Chen Y, Kitchen I, Bailey A
Exercise Attenuates Withdrawal Symptoms From Nicotine In Mice

8th FENS Forum of Neuroscience 2012: Barcelona
Chu Sin Chung P, Keyworth H, Bailey A, Bedford K, Filliol D, Matifas  A, Kitchen I, Beat Lutz and Kieffer B
Dlx-5/6Cre-mediated conditional gene knockout of the delta opioid receptor:  implications on behaviour

8th FENS Forum of Neuroscience 2012: Barcelona
Hambsh B, Keyworth H, Otte DM, Dreslow ML, Kitchen I, Bailey A, Zimmer A
Schizophrenia and comorbid smoking addiction: Impact of chronic nicotine on different forms of memory in G72 transgenic mice

8th FENS Forum of Neuroscience 2012: Barcelona
Farshim P, Bailey A, Chakrabarti B, Swann J, Saddy D, Kitchen I
Delayed Weaning Influences Depressive, But Not Anxiety-like Or Social Behaviour In Rats

IEEE NSS 2012: California
M. Esposito, A. Bailey, J. Newcombe, T. Anaxagoras N. M. Allinson and K. Wells
CMOS APS in pre-clinical science: next generation disruptive technology for multi-modality imaging

British Pharmacological Society Meeting 2011: London
Farshim P, Bailey A, Chakrabarti B, Swann J, Saddy D, Kitchen I
Delayed Weaning Influences Depressive, But Not Anxiety-like Or Social Behaviour In Rats

British Pharmacological Society Meeting 2011: London
Zanos P, Hobson H, Alshehri M, Winsky Sommerer R, Kitchen I, Bailey A
Persistent brain region-specific up-regulation of the vasopressin V1a receptor following chronic cocaine and morphine administration and withdrawal in mice                      

British Pharmacological Society Meeting 2011: London
Smith S, Arlott J, Al Hasani R, Wells L, Hourani S, Kitchen I, Bailey A
Region specific up-regulation of metabotropic mGlu5 receptors in brains of A2A receptor knockout  mice following chronic morphine administration and naloxone-precipitated morphine-withdrawal

World Congress on Neurohypophysial hormones 2011: Boston
P. Zanos, M. Alshehri, T. Sahabandu, R. Winsky-Sommerer, I. Kitchen, A. Bailey
Persistent brain region-specific upregulation of vasopressin (V1ar) and oxytocin receptors in chronic intermittent escalating dose morphine administration in mice

European Opioid Conference 2011: Krakow
A.Bailey
Alterations of the mGluR5, vasopressin and oxytocin receptor systems by chronic opioid administration and withdrawal

European Opioid Conference 2011: Krakow
P. Zanos, M. Alshehri, T. Sahabandu, R. Winsky-Sommerer, I. Kitchen, A. Bailey
Persistent brain region-specific upregulation of vasopressin (V1ar) and oxytocin receptors in chronic intermittent escalating dose morphine administration in mice

European Opioid Conference 2011: Krakow
Smith S, Pochani N, Al Hasani R, Wells L, Hourani S, Kitchen I, Bailey A
Metabotropic mGluR5- And Adenosine A2A- Receptor Interactions In Opioid Addiction

British Pharmacological Society Meeting 2010: London
A.Bailey (Aptuit prize presentation)
Cocaine and opioid addiction and the endogenous opioid system: “A mouse genetics approach to study the neurobiology of drug addiction”

British Pharmacological Society Meeting 2010: London
Smith S, Pochani N, Al Hasani R, Wells L, Hourani S, Kitchen I, Bailey A Metabotropic mGluR5- And Adenosine A2A- Receptor Interactions In Opioid Addiction

International Narcotics Research Conference 2010: Malmo
A. Bailey, V. Viegas, E. Martignoni I. Kitchen
Withdrawal from chronic morphine, but not cocaine induce marked upregulation of mGluR5 binding in the mouse brain

International Narcotics Research Conference 2010: Malmo
A. Bailey, M. Alshehri, T. Sahabandu, I. Kitchen
Withdrawal from chronic morphine induces marked upregulation of V1a vasopressin receptor binding in the mouse brain

British Pharmacological Society Meeting 2009: London
A. Bailey, V. Viegas, E. Martignoni I. Kitchen
Withdrawal from chronic morphine, but not cocaine induce marked upregulation of mGluR5 binding in the mouse brain

British Pharmacological Society Meeting 2009: London
A. Bailey, M. Alshehri, T. Sahabandu, I. Kitchen
Withdrawal from chronic morphine induces marked upregulation of V1a vasopressin receptor binding in the mouse brain

British Pharmacological Society Meeting 2009: London
R. Al Hasani, A. Metaxas, A. Bailey, S. Hourani, I. Kitchen
Differences in dopamine D1 and D2 binding between CD1 mice that respond and those that do not respond to cocain-induced conditioned place preference

Society for Neuroscience meeting 2009:  Chicago
A. Bailey, V. Viegas, E. Martignoni I. Kitchen
Withdrawal from chronic morphine, but not cocaine induce marked upregulation of mGluR5 binding in the mouse brain

14th Annual Meeting of the European-Council-for-Cardiovascular-Research 2009: Nice
Fan, L; Sawbridge, D; Bailey, A, Li, J-M.
The role of oxidative stress in mediating cocaine-induced MAPK activation and cardiac myocyte injuries

British Pharmacological Society Meeting 2008: Brighton
Fan, L., Bailey, A., Kitchen, I., Li, J-M
The role of Nox2 in chronic administration-induced cardiac oxidative stress cardiotoxicity (oral)

British Pharmacological Society Meeting 2008: Brighton
Bailey, A., Metaxas, A., Forster, D.M. and Kitchen., A.
Repeated heroin administration induces locomotion, sensitisation and conditioned place preference in C57BL/6J mice but not in DBA/2J; association with alterations in MOP-r activation and dopamine transporter binding

38th International Narcotics Research Conference 2008: Charleston
Bailey, A., Martignoni, E., Kitchen. I
Persistent region specific upregulation of mGluR5 binding in brains of morphine withdrawn mice

British Cardiovascular Society Conference 2008: Manchester
Fan, L., Sawbridge, D., Bailey, A., Kitchen, I., Li, J-M
The mechanism of chronic cocaine exposure on cardiac reactive oxygen species production and mitogen-activated protein kinase activation

XXVIII European Section Meeting of the International. Society for Heart Research 2008: Athens
Fan, L., Sawbridge, D., Bailey, A., Kitchen, I., Li, J-M
The role of NADPH oxidase in cocaine induced cardiac oxidative stress and redox signalling

European Opioid Conference 2008: Ferrara
Bailey, A., Metaxas, A., Forster, D.M. and Kitchen., A.
Repeated heroin administration induces locomotion, sensitisation and conditioned place preference in C57BL/6J mice but not in DBA/2J; association with alterations in MOP-r activation and dopamine transporter binding

European Opioid Conference 2008: Ferrara
Bailey, A.,  Asari, S. and Kitchen, I.
Complete neuroanatomical mapping of hypocretin (orexin) receptor G protein activation in C57BL/6J mouse brain with the use of [35S]GTPS autoradiography

British Pharmacological  Society Meeting 2007: Brighton
Al-Hasani, R., Bailey, A., Wells, L., Opacka-Juffry, J., Hourani, S., Kitchen, I.
The role of the adenosine A2A receptors in NAc dopamine release and morphine reward

IEEE conference 2007: Hawai
J. Cabello, K. Wells, A. Metaxas, A. Bailey, I. Kitchen, A. Clark, M. Prydderch, R. Turchetta
Digital Autoradiography Imaging Using CMOS Technology: First Tritium Autoradiography with a Back-Thinned CMOS Detector and Comparison of CMOS Imaging Performance with Autoradiography Film

IEEE conference 2007: Hawai
J. Cabello, K. Wells, A. Bailey and I. Kitchen
Semi-automatic elastic registration applied to a-Autoradiography brain atlas

1st Cardiovascular Research Symposium 2007: Guildford
Sawbridge, D., Teng, L., Bailey, A., Ribé, D., George, V., Crossey, P., Kitchen, I. and  Li, J-M.
The Effects of Chronic Cocaine Exposure on Cardiac Reactive Oxygen Species Production and Signalling

37th International Narcotics Research Conference 2007: Berlin
Bailey, A., Metaxas, A., Forster, D.M. and Kitchen., A.
Differences between C57BL/6J and DBA/2J mice in the locomotor,   sensitisation, rewarding and neurochemical effects of chronic heroin treatment on the opioid and dopaminergic systems

37th International Narcotics Research Conference 2007: Berlin
Al-Hasani, R., Bailey, A., Hourani, S., Kitchen, I.
Morphine induced reinstatement in CD1 wildtype and adenosine A2A receptor knockout mice

British Pharmacological  Society Meeting 2006 (75th anniversary Meeting): Oxford, UK
Bailey, A., Yoo, J.H., Racz, I., Zimmer. A., Kitchen. I.
Cocaine sensitization and dopamine D2 and Mu opioid receptor changes in chronic “binge” cocaine treated preprodynorpin knockout mice

36th International Narcotics Research Conference 2006: Minneapolis/St Paul, USA
Bailey, A., Yoo, J.H., Racz, I., Zimmer. A., Kitchen. I.
Enhanced cocaine sensitization in mice lacking preprodynorpin gene

European Opioid Conference 2006: Salamanca, Spain
Bailey, A., Yoo, J.H., Racz, I., Zimmer. A., Kitchen. I.
Enhanced cocaine sensitization in mice lacking preprodynorpin gene

Society for Neuroscience meeting 2006:  Atlanta, USA
Bailey, A., Yoo, J.H., Racz, I., Zimmer. A., Kitchen. I.
Effects of chronic binge cocaine administration on locomotor behaviour, the mu opioid peptide and dopaminergic systems of preprodynorphin knockout mice

35th International Narcotics Research Conference 2005: Annapolis meeting, Maryland, USA
Bailey, A., Gianotti, R., Ho, A. and Kreek, M.J.
Downregulation of  kappa- opioid in brains of  long term withdrawn escalating dose “binge” cocaine treated rats

CPDD meeting 2005: Orlando meeting, Florida, USA
Bailey, A.,  Schlussman, S.D., Ho, A. and Kreek, M.J.
Steady vs Escalating dose chronic “binge” cocaine administration: A preproenkephalin and preprodynorphin gene expression study in the frontal cortex and hippocampus

CPDD meeting 2004: Puerto Rico meeting, San Juan, USA
Bailey, A., Yuferov, V., Bendor, J., Schlussman, S.D., Zhou, Y., Ho, A., Kreek, M.J. Acute withdrawal from chronic “binge” cocaine administration increases -opioid receptor mRNA levels in rat frontal cortex

CPDD meeting 2004: Puerto Rico meeting, San Juan, USA
Schlussman, S.D., Zhou, Y., Bailey, A., Ho, A., Kreek, M.J.
Effect of “binge” pattern and “escalating dose” cocaine administration on striatal preprodynorphin mRNA levels

Society for Neuroscience meeting 2004: San Diego, USA
Schlussman, S.D., Zhou, Y., Bailey, A., Ho, A., Kreek, M.J.
Effects of steady and escalating dose”binge” administration of cocaine on behavioural stereotypy and on striatal preprodynorphin and  opioid receptor mRNA levels in male rats

British Pharmacology Society Meeting 2003: Guildford, Surrey, UK
Bailey, A., Lesscher, H.M.B., Kelly, M., Ledent, C., Davis, L., Hourani, S.M.O.,
Kitchen, I.
Morphine withdrawal in A2A adenosine receptor knockout mice

Neuroscience Conference 2002: Orlando, U.S.A
Lesscher, H.M.B., Bossong, M., van der Linden, A.J.A., Spruijt, B.M., Kitchen, I., Bailey, A., Burbach, J.P.H., van Ree, J.M., Gerrits, M.A.F.M.
Behavioural effects of cocaine and dopamine receptor autoradiography in MOR knockout mice and chronic naltrexone treated mice

Purines Conference 2002: Brisbane, Gold beach, Australia
Bailey, A., Kelly, M., Hourani, S.M.O., Ledent, C., Kitchen, I.
Changes in spinal delta and kappa opioid systems in mice deficient in the A2A receptor gene

European Opioid Conference 2002: Uppsala, Sweeden
Bailey, A., Kelly, M., Hourani, S.M.O., Ledent, C., Kitchen, I.
Changes in spinal delta and kappa  opioid systems in mice deficient in the A2A receptor gene

Drug Discovery 2001: PFIZER global research and development, Sandwich, Kent, UK.
Bailey, A., Kelly, M., Hourani, S.M.O., Ledent, C., Kitchen, I. Quantitative autoradiography of mu, delta, kappa, ORL-1 and A1 receptors in the brains and spinal cords of A2A adenosine receptor knockout mice

32nd International Narcotics Research Conference 2001: Helsinki,
Bailey, A., Kelly, M., Hourani, S.M.O., Ledent, C., Kitchen, I. Quantitative autoradiography of mu, delta, kappa, ORL-1 and A1 receptors in the brains and spinal cords of A2A adenosine receptor knockout mice

British Pharmacology Society Meeting 2001: Bristol meeting, Bristol, UK
Toms, N.J., Bailey, A., Kelland, E.E., Thomas, A., Biggs, J., Crawford, D., Kitchen, I.,  Regional localisation of low-affinity kainate receptors in murine brain using [3H](2S,4R)-4-methylglutamate autoradiography

30th International Narcotics Research Conference 1999: Sarantoga Springs, New York
Oakley, S.M., Bailey, A., Matthes, H., Kieffer, B., Toth, G., Borsodi, A., Kitchen, I.
Autoradiographic characterisation of G-protein coupling of delta-opioid receptors in the brains of mu-receptor knockout mice

Teaching

Undergraduate
Biochemistry 1 (BMS1030)
Pharmacology 1 (BMS2047)
Neuroscience 1 (BMS2048)
Neuroscience 2 (BMS3064)

MSc
Principles in Toxicology (TOX 002)
Practical Toxicology (TOX007)
Experimental Psychology (PSYM022)
MSc Nutritional Medicine
MSc Palliative care
MSc Health and Clinical Sciences
CPD MSc Clinical Pharmacology
University Sao Paulo (invited lectures)

Project Supervision
Supervision of BSc, MSc, Erasmus and PhD research projects

Departmental Duties

Senior Tutor, Professional Training (since 2013)
Level 5 organizer (since 2009)

Affiliations

British Pharmacology Society
Society for Neuroscience
New York Academy of Sciences
Rockefeller University Post-doctoral Association

European Opioid Conference

International Narcotics Research Conference

External Responsibilities

Editorial board member, British Journal of Pharmacology (since 2013)
Co-organizer of the European Opioid Conference (2013)
Chair of Junior scientists prize symposium, European Opioid Conference (2013)
Poster approval referee for European Opioid Conference and Festival of Research (2013)
Member of the list of expert’s database of the Science Media Centre (since 2009)
Peer review of grants for multiple scientific bodies
Peer review of manuscripts for multiple journals

Outreach and Media

School talks on drug addiction
Organizer or taster course in Neuroscience
Interview with the BPS president (Ray Hill) about my research 
BBC and National Geographic documentary on the effects of cocaine 

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