DNA Microarrays
Arrays of known stretches of DNA on solid supports representing the entire genome can be used as "probes" for complementary labelled nucleic acids in solution. DNA arrays are used to determine global gene expression patterns from RNA preparations or global distribution of transcription factor binding sites through 'ChIP-on-chip'. Hybridisation of labelled genomic DNA from diverse strains or species on a microarray, called comparative genomic hybridisation (CGH) can reveal the genetic basis of the diversity. In accordance with the international nomenclature we have used "probe" to refer to the DNA immobilised on a solid surface and "target" the labelled DNA in solution.
Services
We offer training in all practical aspects of DNA microarray experiments for both prokaryotic and eukaryotic systems. The array platform preferentially used in our laboratory is the Agilent platform; however we can print “custom” spotted arrays on request.
We are a worldwide resource for spotted Oligo, PCR-product and high density ink-jet in situ synthesised Streptomyces arrays.
Please see our Microarray resources site for further details.
Instruments
- Nanodrop ND-1000 spectrophotometer
- Agilent Bioanalyser
- Agilent scanner (with XDR)
- Axon 4000B scanner
- Genetix Q-array2 printing robot
- MWG Liquid handling robot and PCR robotics
- Qiagen BioRobot Rapid Plate and Twister arm
- MAUI hybridization station (12X)
- Agilent hybridization oven
Costs
All instruments in the DNA microarray facility are available to University members prior arrangements. There is however a cost associated with using our equipment. Please contact microarrays@surrey.ac.uk for further information.
Microbial Sciences Division,
Faculty of Health and Medical Sciences,
University of Surrey,
Guildford, GU2 7XH, UK.
Tel. 01483 686498
