Professor Michael Bushell

Journal articles

• Ahmed AE-SI, Cavalli G, Bushell ME, Wardell JN, Pedley S, Charles K, Hay JN. (2011) 'New Approach To Produce Water Free of Bacteria, Viruses, and Halogens in a Recyclable System'. AMER SOC MICROBIOLOGY APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77 (3), pp. 847-853.
  doi: 10.1128/AEM.01645-10
  Full text is available at: http://epubs.surrey.ac.uk/7417/
• Ahmed AE-SI, Wardell JN, Thumser AE, Avignone-Rossa CA, Cavalli G, Hay JN, Bushell ME. (2011) 'Metabolomic Profiling Can Differentiate Between Bactericidal Effects of Free and Polymer Bound Halogen'. WILEY-BLACKWELL JOURNAL OF APPLIED POLYMER SCIENCE, 119 (2), pp. 709-718.
  doi: 10.1002/app.32731
• Lewis RA, Shahi SK, Laing E, Bucca G, Efthimiou G, Bushell M, Smith CP. (2011) 'Genome-wide transcriptomic analysis of the response to nitrogen limitation in Streptomyces coelicolor A3(2).'. BMC Res Notes, England: 4
  doi: 10.1186/1756-0500-4-78
  Full text is available at: http://epubs.surrey.ac.uk/203297/

  Abstract

  The present study represents a genome-wide transcriptomic analysis of the response of the model streptomycete Streptomyces coelicolor A3(2) M145 to fermentor culture in Modified Evans Media limited, respectively, for nitrogen, phosphate and carbon undertaken as part of the ActinoGEN consortium to provide a publicly available reference microarray dataset.

• Sroka J, Bieniasz-Krzywiec L, Gwóźdź S, Leniowski D, Lącki J, Markowski M, Avignone-Rossa C, Bushell ME, McFadden J, Kierzek AM. (2011) 'Acorn: a grid computing system for constraint based modeling and visualization of the genome scale metabolic reaction networks via a web interface.'. BioMed Central BMC Bioinformatics, England: 12 (196)
  doi: 10.1186/1471-2105-12-196
  Full text is available at: http://epubs.surrey.ac.uk/185976/

  Abstract

  Constraint-based approaches facilitate the prediction of cellular metabolic capabilities, based, in turn on predictions of the repertoire of enzymes encoded in the genome. Recently, genome annotations have been used to reconstruct genome scale metabolic reaction networks for numerous species, including Homo sapiens, which allow simulations that provide valuable insights into topics, including predictions of gene essentiality of pathogens, interpretation of genetic polymorphism in metabolic disease syndromes and suggestions for novel approaches to microbial metabolic engineering. These constraint-based simulations are being integrated with the functional genomics portals, an activity that requires efficient implementation of the constraint-based simulations in the web-based environment.

• Gevorgyan A, Bushell ME, Avignone-Rossa C, Kierzek AM. (2010) 'SurreyFBA: a command line tool and graphics user interface for constraint-based modeling of genome-scale metabolic reaction networks'. OXFORD UNIV PRESS BIOINFORMATICS, 27 (3), pp. 433-434.
  doi: 10.1093/bioinformatics/btq679
• Ahmed AE-SI, Hay JN, Bushell ME, Wardell JN, Cavalli G. (2010) 'Macroscopic N-Halamine Biocidal Polymeric Beads'. JOHN WILEY & SONS INC JOURNAL OF APPLIED POLYMER SCIENCE, 116 (4), pp. 2396-2408.
  doi: 10.1002/app.31774
• Ahmed AE-SI, Hay JN, Bushell ME, Wardell JN, Cavalli G. (2009) 'Optimizing Halogenation Conditions of N-Halamine Polymers and Investigating Mode of Bactericidal Action'. JOHN WILEY & SONS INC JOURNAL OF APPLIED POLYMER SCIENCE, 113 (4), pp. 2404-2412.
  doi: 10.1002/app.30390
• Ahmed AE-SI, Hay JN, Bushell ME, Wardell JN, Cavalli G. (2008) 'Biocidal polymers (II): Determination of biological activity of novel N-halamine biocidal polymers and evaluation for use in water filters'. ELSEVIER SCIENCE BV REACTIVE & FUNCTIONAL POLYMERS, 68 (10), pp. 1448-1458.
  doi: 10.1016/j.reactfunctpolym.2008.06.021
• Khannapho C, Zhao H, Bonde BK, Kierzek AM, Avignone-Rossa CA, Bushell ME. (2008) 'Selection of objective function in genome scale flux balance analysis for process feed development in antibiotic production'. ACADEMIC PRESS INC ELSEVIER SCIENCE METABOLIC ENGINEERING, 10 (5), pp. 227-233.
  doi: 10.1016/j.ymben.2008.06.003
• Ahmed AEI, Hay JN, Bushell ME, Wardell JN, Cavalli G. (2008) 'Biocidal polymers (I): Preparation and biological activity of some novel blocidal polymers based on uramil and its azo-dyes'. ELSEVIER SCIENCE BV REACTIVE & FUNCTIONAL POLYMERS, 68 (1), pp. 248-260.
  doi: 10.1016/j.reactfunctpolym.2007.09.004
• Beste DJV, Laing E, Bonde B, Avignone-Rossa C, Bushell ME, McFadden JJ. (2007) 'Transcriptomic analysis identifies growth rate modulation as a component of the adaptation of mycobacteria to survival inside the macrophage'. AMER SOC MICROBIOLOGY JOURNAL OF BACTERIOLOGY, 189 (11), pp. 3969-3976.
  doi: 10.1128/JB.01787-06
  Full text is available at: http://epubs.surrey.ac.uk/184935/
• Beste DJV, Hooper T, Stewart G, Bonde B, Avignone-Rossa C, Bushell M, Wheeler P, Klamt S, Kierzek AM, McFadden J. (2007) 'GSMN-TB: a web-based genome scale network model of Mycobacterium tuberculosis metabolism'. BIOMED CENTRAL LTD GENOME BIOLOGY, 8 (5) Article number ARTN r89
  doi: 10.1186/gb-2007-8-5-r89
  Full text is available at: http://epubs.surrey.ac.uk/184904/
• Bushell ME, Sequeira SIP, Khannapho C, Zhao H, Chater KF, Butler MJ, Kierzek AM, Avignone-Rossa CA. (2006) 'The use of genome scale metabolic flux variability analysis for process feed formulation based on an investigation of the effects of the zwf mutation on antibiotic production in Streptomyces coelicolor'. ELSEVIER SCIENCE INC ENZYME AND MICROBIAL TECHNOLOGY, 39 (6), pp. 1347-1353.
  doi: 10.1016/j.enzmictec.2006.06.011
• Rozkov A, Avignone-Rossa CA, Ertl PF, Jones P, O'Kennedy RD, Smith JJ, Dale JW, Bushell ME. (2006) 'Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1'. ELSEVIER SCIENCE INC ENZYME AND MICROBIAL TECHNOLOGY, 39 (1), pp. 47-50.
  doi: 10.1016/j.enzmictec.2005.09.005
• Bushell ME, Kirk S, Zhao HJ, Avignone-Rossa CA. (2006) 'Manipulation of the physiology of clavulanic acid biosynthesis with the aid of metabolic flux analysis'. ELSEVIER SCIENCE INC ENZYME AND MICROBIAL TECHNOLOGY, 39 (1), pp. 149-157.
  doi: 10.1016/j.enzmictec.2006.01.017
• Beste DJ, Peters J, Hooper T, Avignone-Rossa C, Bushell ME, McFadden J. (2005) 'Compiling a molecular inventory for Mycobacterium bovis BCG at two growth rates: evidence for growth rate-mediated regulation of ribosome biosynthesis and lipid metabolism.'. J Bacteriol, United States: 187 (5), pp. 1677-1684.
  doi: 10.1128/JB.187.5.1677-1684.2005
  Full text is available at: http://epubs.surrey.ac.uk/184910/

  Abstract

  An experimental system of Mycobacterium tuberculosis growth in a carbon-limited chemostat has been established by the use of Mycobacterium bovis BCG as a model organism. For this model, carbon-limited chemostats with low concentrations of glycerol were used to simulate possible growth rates during different stages of tuberculosis. A doubling time of 23 h (D = 0.03 h(-1)) was adopted to represent cells during the acute phase of infection, whereas a lower dilution rate equivalent to a doubling time of 69 h (D = 0.01 h(-1)) was used to model mycobacterial persistence. This chemostat model allowed the specific response of the mycobacterial cell to carbon limitation at different growth rates to be elucidated. The macromolecular (RNA, DNA, carbohydrate, and lipid) and elemental (C, H, and N) compositions of the biomass were determined for steady-state cultures, revealing that carbohydrates and lipids comprised more than half of the dry mass of the BCG cell, with only a quarter of the dry weight consisting of protein and RNA. Consistent with studies of other bacteria, the specific growth rate impacts on the macromolecular content of BCG and the proportions of lipid, RNA, and protein increased significantly with the growth rate. The correlation of RNA content with the growth rate indicates that ribosome production in carbon-limited M. bovis BCG cells is subject to growth rate-dependent control. The results also clearly show that the proportion of lipids in the mycobacterial cell is very sensitive to changes in the growth rate, probably reflecting changes in the amounts of storage lipids. Finally, this study demonstrates the utility of the chemostat model of mycobacterial growth for functional genomic, physiology, and systems biology studies.

• Rozkov A, Avignone-Rossa CA, Ertl PF, Jones P, O'Kennedy RD, Smith JJ, Dale JW, Bushell ME. (2004) 'Characterization of the metabolic burden on Escherichia coli DH1 cells imposed by the presence of a plasmid containing a gene therapy sequence'. JOHN WILEY & SONS INC BIOTECHNOLOGY AND BIOENGINEERING, 88 (7), pp. 909-915.
  doi: 10.1002/bit.20327
• Bushell ME, Rowe M, Avignone-Rossa CA, Wardell JN. (2003) 'Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris'. JOHN WILEY & SONS INC BIOTECHNOLOGY AND BIOENGINEERING, 82 (6), pp. 678-683.
  doi: 10.1002/bit.10616
• Wardell JN, Stocks SM, Thomas CR, Bushell ME. (2002) 'Decreasing the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338 leads to increased resistance to breakage and increased antibiotic production.'. Biotechnol Bioeng, United States: 78 (2), pp. 141-146.
• Kirk S, Avignone-Rossa CA, Bushell ME. (2000) 'Growth limiting substrate affects antibiotic production and associated metabolic fluxes in Streptomyces clavuligerus'. KLUWER ACADEMIC PUBL BIOTECHNOLOGY LETTERS, 22 (22), pp. 1803-1809.
  doi: 10.1023/A:1005670603596
• Dunstan GH, Avignone-Rossa C, Langley D, Bushell ME. (2000) 'The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic.'. Enzyme Microb Technol, 27 (7), pp. 502-510.
• Wardell JN, Bushell ME. (1999) 'Kinetics and manipulation of hyphal breakage and its effect on antibiotic production'. ELSEVIER SCIENCE INC ENZYME AND MICROBIAL TECHNOLOGY, 25 (3-5), pp. 404-410.
  doi: 10.1016/S0141-0229(99)00064-2
• Bushell ME, Bull AT. (1999) 'Sporulation at minimum specific growth rate in Aspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations.'. J Basic Microbiol, GERMANY: 39 (5-6), pp. 293-298.
• Ives PR, Bushell ME. (1997) 'Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus.'. Microbiology, ENGLAND: 143 ( Pt 11), pp. 3573-3579.
• Moore J, Bushell ME. (1997) 'The effect of morphology and oxygen uptake on penicillin production by Aspergillus nidulans in submerged culture'. CAMBRIDGE UNIV PRESS MYCOLOGICAL RESEARCH, 101, pp. 1237-1241.
  doi: 10.1017/S0953756297003882
• Bushell ME, Dunstan GL, Wilson GC. (1997) 'Effect of small scale culture vessel type on hyphal fragment size and erythromycin production in Saccharopolyspora erythraea'. CHAPMAN HALL LTD BIOTECHNOLOGY LETTERS, 19 (9), pp. 849-852.
  doi: 10.1023/A:1018325301500
• Bushell ME, Smith J, Lynch HC. (1997) 'A physiological model for the control of erythromycin production in batch and cyclic fed batch culture'. SOC GENERAL MICROBIOLOGY MICROBIOLOGY-UK, 143, pp. 475-480.
• Martin SM, Bushell ME. (1996) 'Effect of hyphal micromorphology on bioreactor performance of antibiotic-producing Saccharopolyspora erythraea cultures'. SOC GENERAL MICROBIOLOGY MICROBIOLOGY-UK, 142, pp. 1783-1788.
• Lynch HC, Bushell ME. (1995) 'The physiology of erythromycin biosynthesis in cyclic fed batch culture'. SOC GENERAL MICROBIOLOGY MICROBIOLOGY-UK, 141, pp. 3105-3111.