Blackbourn laboratory methods
2
A
B
- BACK50, batch testing of
- BACK50, luciferase testing of
- BACK50, production of
- BAC recombination protocol using galK
- Bac-to-Bac Baculovirus Expression System (Invitrogen)
- BCBL-1 cells, reactivation of
- B cell transfection by electroporation
- Buffer E for cell lysis (Western blotting)
C
- Calibrating the CO2 incubator
- CAT reporter assay
- Citrate antigen retrieval buffer
- Complement attack assay
- Complement attack assay (revised)
- Coomassie Blue staining of SDS-PAGE gels
- Culture of tDMVEC
D
- DNA loading buffer
- DNA probes, labelling for Southern and Northern blotting
- DNAse treatment
- Dual luciferase assay
E
- Electrophoretic mobility shift assay (EMSA)
- Endothelial cell medium
- Enhanced chemiluminescence (ECL) reagents
- Enzyme-linked immunosorbent assay (ELISA)
F
G
H
- Haematoxylin and eosin (H&E) staining of tissues section
- Harvesting cells for luciferase assay
- Harvesting dishes of tDMVEC cells
- HeLa transfections
- Homemade chemically competent E.coli
- Homemade chemically competent E.coli, transformation of plasmid into
- Hybridisation of Hybond nylon membrane (Amersham)
I
- IFA acetone/methanol fixation
- Immunocytochemistry
- Immunoprecipitation
- In-gel digestion of Coomassie-stained protein
- Intracellular IFN assay
K
L
M
N
O
P
- PBMC preps for buffying T cell clones
- PCR primer dissolution
- Permanent plasmid stocks
- Phenol extraction
- Plasmid Purification
- Plasmids on filter paper
- PolyA mRNA by oligo(dT) bulk preparation
- Preparation of EMSA probes
- Prepartation of competent JM109 E.coli cells
- Proteinase K treatment of PCR products for cloning
R
S
- SDS-PAGE gels
- SDS-PAGE loading buffer
- SF9 insect cells, growth of
- Shipping instructions for sera
- Shipping instructions for virus
- Silver staining of gels
- STM cell lysis
T
- TAE buffer, 50x
- TaqMan
- T cell clone culture medium
- T cell clonning with antigen specific tetramers
- T cells, IFN assay
- T cells, staining for CD200R
- Total RNA purification
- Transformation of competent cells
- TSA simple amplification
- Tumour infiltrating lymphocyte preparation methods