Nox enzymes in aging bladder dysfunction
Aims and objectives
The overall aim of this collaborative project is to investigate the role of Nox enzymes and ROS in regulating bladder function and mechanisms of Nox activation in ageing bladders. We will first determine whether Nox enzymes exist in the urothelium and bladder; if so what subtypes they are; next whether these Nox subtypes are functional and their characteristics and which is the main functional subtype(s); we will then investigate how these Nox and associated ROS affect normal urothelium and bladder physiology and next how they contribute to urothelium and bladder dysfunction in response to ageing process; finally we will examine the mechanisms whereby these Nox pathways work at cellular and molecular levels.
This is the first study to examine the role of Nox and ROS in urothelial function. The outcome of this project is expected to identify a novel regulator, Nox molecules, in the newly-recognized sensory structure – the urothelium, and provide new insight into bladder aging and bladder dysfunction in the humans. It will also provide added translational value for the use of novel specific Nox inhibitors in improving aging bladder function and the potential for treating bladder disorders. Providing scientific basis for instillation of Nox inhibitors in the bladder in clinical practice.