Bioimaging and flow cytometry

3 lasers and 6 laser lines:

1. Argon: 458nm, 477nm , 488nm ,514nm
2. HeNe1: 543nm
3. HeNe2: 633nm
4. META spectral detector for spectral imaging

Objective lenses:

1. Plan-Neofluar 10x/0.3
2. Plan-Neofluar 20x/0.5NA
3. Plan-Neofluar 40x/1.3NA oil DIC
4. C-Apochromat 63x/1.2 W corr
5. Plan-Apochromat 63x/1.4 oil DIC

• Motorised stage for automated multi area timelapse imaging
• Full environmental chamber for temperature control plus local CO2 delivery for atmospheric control
• Windows Vista computer with Zen 2009 confocal acquisition software

4 lasers and 6 laser lines:

1. High power solid state laser: 405nm
2. Argon: 457nm, 488nm ,514nm
3. Solid state diode laser, Coherent: 561nm
4. Solid state diode laser, Melles Griot: 643nm

• Mercury lamp for Widefield fluorescence imaging
• Nikon spectral detector for spectral imaging

Objective lenses:

For Brightfield, Fluorescence and Phase Contrast

• Plan Fluor 10x/0.3NA Working distance 15.2mm DL
• Plan Fluor (Extra Long Working Distance – for imaging through well plates) 20x/0.45NA Working distance 8.2mm ADM
• Plan Fluor (Extra Long Working Distance – for imaging through well plates) 40x/0.60NA Working distance 3.6mm ADM

For Brightfield, Fluorescence and Differential Interference Contrast (DIC)

1. Plan Apo Violet corrected 20x/0.75NA Working distance 1mm DIC
2. Plan Apo Violet corrected 60x/1.40NA Working distance 0.13mm Oil DIC

• Motorised stage for automated multi area timelapse imaging
• Full environmental chamber for temperature control plus local CO₂ delivery for atmospheric control
• Windows 7 computer with NIS elements acquisition software for both confocal and widefield imaging

3 LEDS:

1. Ex: 405nm Em: 447/60 (DAPI)
2. Ex: 488nm Em: 510/42 (GFP)
3. Ex: 585nm Em: 624/40 (Texas Red)

• Transmitted light (bright field and phase contrast)

Objective lenses:

1. Plan Fluor (Extra Long Working Distance – for imaging through well plates / slides) 40x/0.65NA Working distance 2.8mm
2. Plan Apo 100x/1.40NA Working distance 0.13mm Oil DIC

• 15” LCD display high-resolution (1,024 x 768 pixels) colour monitor with adjustable tilt
• EVOS FL software for easy snapshot images of fluorescent samples straight to USB stick. Time lapse and cell counting functions are also available.