Bioimaging and flow cytometry

The University of Surrey Bioimaging and Flow Cytometry (BIAFC) core facility supports the imaging and flow cytometry requirements of staff and students at the University of Surrey, as well as to outside academic and industrial institutions upon request.

Make an enquiry

Email us

About us

We have a wide research user base, with particular interests in:

Cell biology
Immunology
Bacteriology
Virology
Cancer biology
Cell death

We provide full training and any assistance required in flow cytometry and confocal microscopy. We also offer a cell sorting service.

Flow cytometry

The instruments within the core facility allow for multi-parameter analysis of your cells, including eukaryotic and prokaryotic cells which can either be alive or fixed. It is also possible to sort your cells into separate populations based on their characteristics as shown by fluorescent indicators.

See below for further details on the capabilities of the instruments within the facility to enable fluorochrome choice.

The facility is equipped with the following flow cytometers and cell sorter:

BD Biosciences FACS ARIA Fusion (4-laser, 16 colour), FACS DIVA v8.0 software - contained in a Class II Microbiology Safety Cabinet
BD Biosciences FACS Celesta (3-laser, 12-colour),FACS DIVA v8.0 software
Thermo Fisher Attune Acoustic Focussing Flow Cytometer (2-laser, 7-colour), Attune NxT software
Analysis computer containing FlowJo v10 software.

Laser	Filters	Fluorochrome Choices
Blue 488nm	530/30	FITC, GFP
	590/40	PI
	695/40	PerCP-Cy5.5
Yellow/Green 561nm	585/16	PE, RFP
	620/15	mCherry, PE-Texas Red
	695/40	PE-Cy5.5
	780/60	PE-Cy7

Bioimaging

The instruments that are available allow for multi-dimensional analysis of your cells including eukaryotic and prokaryotic cells. Analysis is based on fluorescence and brightfield imagery, and samples can either be alive or fixed. See link below for further details on the capabilities of the instruments within the facility to enable fluorochrome choice and experimental design.

The facility is equipped with the following microscopes:

Zeiss LSM510 VIS META Confocal Microscope (on an Axiovert 200M microscope)
Nikon A1M Confocal Microscope and DS-Qi1 Widefield Camera (on an Eclipse Ti-E Microscope)
Life Technologies EVOS FL widefield microscope.

Book equipment and request training

Please direct requests for equipment booking and training to:

Gillian Wallis, Research Laboratory Manager
Tel: +44 (0)1483 689755
Email: g.wallis@surrey.ac.uk

Laser	Filters	Fluorochrome Choices
Violet 405nm	450/50	BV421, V450, Pacific Blue, DAPI
	525/50	BV510, V500, AmCyan
	610/20	BV605
	660/20	BV650
	710/50	BV711
	780/60	BV786
Blue 488nm	530/30	FITC, AF488, GFP, BB515
	695/40	PerCP-Cy5.5
Yellow/Green 561nm	582/15	PE, DsRed, RFP
	610/20	FITC, AF488, GFP, BB515
	670/14	PE-Cy5
	710/50	PE-Cy5.5
	780/60	PE-Cy7
Red 640nm	670/30	APC, AF647
	730/45	AF700
	780/60	APC-Cy7, APC-H7

Laser	Filters	Fluorochrome Choices
Violet 403nm	450/50	BV421, V450, Pacific Blue, DAPI
	525/50	BV510, V500, AmCyan
	610/20	BV605
	670/30	BV650
	780/60	BV786
Blue 488nm	530/30	FITC, AF488, GFP, BB515
	575/25	PE
	610/20	FITC, AF488, GFP, BB515
	695/40	PerCP-Cy5.5
Red 640nm	670/30	APC, AF647
	730/45	AF700
	780/60	APC-Cy7, APC-H7

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Bioimaging and flow cytometry

Make an enquiry

About us

BD Biosciences FACS ARIA Fusion (4-laser, 16 colour), with FACS DIVA v8.0

BD Biosciences FACS Celesta (3-laser, 12 colour), with FACS DIVA v8.0

ThermoFisher Attune Acoustic Focussing Flow Cytometer (2-laser, 7 colour), with Attune Software v2.4

Zeiss LSM510 VIS META Confocal Microscope (on an Axiovert 200M microscope)

Nikon A1M Confocal Microscope and DS-Qi1 Widefield Camera (on an Eclipse Ti-E Microscope)

Life Technologies EVOS FL Microscope

Book equipment and request training

Useful links