Persistent Salmonella Newport contamination was found within rearing houses and on their external concrete aprons after cleaning and disinfection between crops of heavily shedding young birds.
Salmonella shedding was often detected by 5 d of age and the frequency of positive samples peaked at 14?35 d. Thereafter Salmonella isolations declined, especially in the later (fattening) stages. Samples were still Salmonella-positive at low prevalence in half of the intensively sampled houses at slaughter age.
A number of management interventions to combat Salmonella infection of flocks, including sourcing policy, competitive exclusion cultures and cleaning and disinfection, were inadequate to prevent flock infection, although improved disinfection on one unit was associated with a delay in the onset of flock infection.
Extends the breadth and coverage of the first volume in the series
Includes updates on specific pathogens and safety for specific foods
Reviews both detection and management of foodborne pathogens
sows and gilts on three commercial pig units experiencing clinical salmonellosis associated with
S. Typhimurium or its monophasic variant. After vaccination, clinical salmonellosis resolved and
shedding of S. Typhimurium declined markedly and persistently on all breeding or breedingfinishing
units, during the one- to two-year monitoring period. On two finishing units supplied in
part by one of the vaccinated herds, pigs from the vaccinated herd were less likely to shed
Salmonella than those from non-vaccinating herds, and Salmonella counts in faeces were also lower
from the vaccine-linked animals. Non-Typhimurium Salmonella serovars were isolated typically in
fewer than 10% of samples, and showed no clear temporal changes in frequency. Vaccination of
dams alone with S. Typhimurium was associated with reduced shedding of closely-related serovars
among all age groups in this commercial setting.
commercial formulations of organic acid products, Avian Pathology 42 (3) pp. 268-275 Taylor & Francis
a cause of substantial concern among food safety bodies, prompting voluntary and legislative
responses aimed at monitoring and reducing the number of Salmonella-infected animals
entering the human food chain. Elimination of the problem at an early stage of production is
highly desirable, and to this end the present review examines published evidence on the
carriage of Salmonella by piglets before and after weaning, plus evidence on the dynamics of
Salmonella infection in the weaner and grower stages of pig production, the effects of
maternal immunity, and risk factors for Salmonella excretion after weaning. Various
interventions to reduce or eliminate Salmonella infection in young pigs have been tried, such
as vaccination, competitive exclusion, feed and water treatments, antibiotic administration,
disinfection of animals, and segregated weaning to clean accommodation. The evidence on
the effectiveness of these is considered, and the last is examined in some detail as it appears
presently to offer the best chance of eliminating Salmonella from growing stock.
in E. coli and thermophilic Campylobacter spp. on
poultry farms, Avian Pathology 45 (5) pp. 559-568 Taylor & Francis
during a cross-sectional survey of 89 poultry meat farms in England and Wales to
provide information on possible risk factors for the occurrence of fluoroquinolone
(FQ) resistant bacteria. Faeces samples were used to classify farms as ?affected? or
?not affected? by FQ-resistant E. coli or Campylobacter spp. Risk factor analysis
identified the use of FQ on the farms as having by far the strongest association,
among the factors considered, with the occurrence of FQ-resistant bacteria.
Resistant E. coli and/or Campylobacter spp. were found on 86% of the farms with a
history of FQ use. However, a substantial proportion of farms with no history of FQ
use also yielded FQ-resistant organisms, suggesting that resistant bacteria may
transfer between farms. Further analysis suggested that for Campylobacter spp., onfarm
hygiene, cleaning and disinfection between batches of birds and wildlife control
were of most significance. By contrast, for E. coli biosecurity from external
contamination was of particular importance, although the modelling indicated that
other factors were likely to be involved. Detailed studies on a small number of sites
showed that FQ-resistant E. coli can survive routine cleaning and disinfection. It
appears difficult to avoid the occurrence of resistant bacteria when FQ are used on a
farm, but the present findings provide evidence to support recommendations to
reduce the substantial risk of the incidental acquisition of such resistance by farms
where FQ are not used.
an Integrated Pig Breeding and Production Organisation in
the United Kingdom, ISRN Veterinary Science 2013 943126 Hindawi Publishing Corporation
faeces samples from 161 nursery and finishing units in a UK integrated pig enterprise. An epidemiological questionnaire was also
completed by investigators for each farm. Salmonella was isolated from 630 (19.5%) of the samples: S. Typhimurium was found
in 387 (12%) and S. Derby in 157 (4.9%) samples; 111 units yielded at least one sample containing Salmonella. The proportion of
Salmonella-positive samples from positive farms ranged from 5% to 95%. In a univariable risk factor analysis, increasing length of
time as a pig farm was positively associated with the detection of Salmonella in a herd. Larger farms (>500 pigs) were significantly
more likely to be positive for S. Typhimurium than smaller farms. There was an association between Salmonella serovars isolated
in the present study and those subsequently isolated in breeding herds linked to the integration.
hens., Avian Pathology 40 (5) pp. 429-436 Taylor & Francis
the World, but where Salmonella Enteritidis is endemic it is this latter serovar that
dominates egg-borne salmonellosis. However, Salmonella Typhimurium is a major
foodborne pathogen so it is important to understand how it can impact the
microbiological safety of eggs and what serovar-specific control strategies may be
appropriate in the future as control over Salmonella Enteritidis continues to
improve. To that end, the present review examines the published literature on
Salmonella Typhimurium in laying hens and eggs, with particular reference to
comparative studies examining different serovars. Experimentally Salmonella
Enteritidis is more often isolated from egg contents and seems to adhere better to
reproductive tract mucosa, whilst Salmonella Typhimurium appears to provoke a
more intense tissue pathology and immune response, and flock infections are more
transient. However, it is observed that in many cases the present body of evidence
does not identify clear differences between specific behaviours of the serovars
Typhimurium and Enteritidis, whether in laying hens, in their eggs, or in the laying
environment. It is concluded that further long-term experimental and natural
infection studies are needed in order to generate a clearer picture.
Significance and Impact of the Study
Salmonella surveillance and control regimes in the European poultry industry and elsewhere require sensitive culture detection of Salmonella in environmental samples, including poultry faeces. A ring trial was conducted, and the results highlighted that some of the participating laboratories failed to identify Salmonella. This suggests that contaminated frozen faeces cubes could be beneficial to assess proficiency, according to the results of this preliminary study. The data obtained in this study can be used as an indication for the design of realistic external quality assurance for laboratories involved in official testing of Salmonella in poultry flocks.
feedmills in the UK, Journal of Applied Microbiology 109 (4) pp. 1430-1440 Wiley
To investigate and compare commercial and farm-level milling operations in
respect of the monitoring and control of Salmonella contamination.
Methods and Results:
Four commercial feedmills and four on-farm poultry feed
mixers were intensively sampled. Samples included dust and spillages and were
cultured for Salmonella. Serovars in ingredients on farms were associated with wildlife
and/or livestock, whereas those in commercial mill ingredients were associated with
domestically-produced cereals and imported vegetable protein. Endemic
contamination of two commercial feedmills was reflected in isolates obtained from
finished products and destination flocks. Renovation of equipment and chemical
treatment of equipment and feed had not removed endemic strains, and previous
routine monitoring in the commercial mills had not revealed the degree of
contamination found in the present investigations.
Ingredient contamination was diverse and reflected the sources and
storage environments used by mills and farms, respectively. The use of dust and
spillage samples showed a clear sensitivity advantage over the previously-used
monitoring methods in the feedmills.
Significance and Impact of Study:
Monitoring for Salmonella contamination of
commercial feedmills requires sensitive methods, such as those employed in the
present study. This is particularly important for endemic contamination.
arthropods, with special reference to Salmonella in
mites, flies and litter beetles, Zoonoses and Public Health 57 (5) pp. 299-314 Wiley
found on and around livestock premises, and zoonotic bacteria. The principal focus is
upon insects and arachnids on poultry units, where houses, litter and manure provide
good conditions for the growth, multiplication and protection of flies, beetles and
mites, and where zoonotic pathogens such as Salmonella and Campylobacter are
prevalent. Other members of the Enterobacteriaceae and the taxa Clostridium,
Helicobacter, Erysipelas and Chlamydiaceae are also discussed. Salmonella is widely
distributed in the flies of affected livestock units, and is detectable to a lesser degree
in beetles and mites. Persistent carriage appears to be common and there is some field
and experimental evidence to support arthropod-mediated transmission between
poultry flocks, particularly carry-over from one flock to the next. Campylobacter may
readily be isolated from arthropods in contact with affected poultry flocks, although
carriage is short-lived. There appears to be a role for flies, at least, in the breaching of
biosecurity around Campylobacter-negative flocks. The carriage of other zoonotic
bacteria by arthropods has been documented, but the duration and significance of such
associations remain uncertain in the context of livestock production.
Heavy Metals, and Its Relevance to Foodborne Pathogens, Antibiotics 4 (4) pp. 567-604 MDPI
resistance among bacteria exposed to biocides used as disinfectants, antiseptics and
preservatives, and to heavy metals (particularly copper and zinc) used as growth promoters
and therapeutic agents for some livestock species. There is indeed experimental and
observational evidence that exposure to these non-antibiotic antimicrobial agents can induce
or select for bacterial adaptations that result in decreased susceptibility to one or more
antibiotics. This may occur via cellular mechanisms that are protective across multiple
classes of antimicrobial agents or by selection of genetic determinants for resistance to
non-antibiotic agents that are linked to genes for antibiotic resistance. There may also be
relevant effects of these antimicrobial agents on bacterial community structure and via
non-specific mechanisms such as mobilization of genetic elements or mutagenesis. Notably,
some co-selective adaptations have adverse effects on fitness in the absence of a continued
selective pressure. The present review examines the evidence for the significance of these
phenomena, particularly in respect of bacterial zoonotic agents that commonly occur in
livestock and that may be transmitted, directly or via the food chain, to human populations.
distribution of Salmonella in the environment
of caged layer flocks, Journal of Applied Microbiology 101 (2) pp. 309-318 Wiley
To evaluate a semi-quantitative technique for the enumeration of Salmonella in the
environment of layer flocks, and to compare findings with those of a standard qualitative
Methods and Results:
Samples were taken from faeces, floor dust, dust on cages, feeders
and egg belts. After mixing with buffered peptone water, serial dilutions were prepared and
culture was performed using pre-enrichment, then plating on semi-solid selective and solid
isolation media. Comparison with a qualitative pre-enrichment technique indicated a similar
sensitivity for both methods despite smaller sample sizes. The numbers of Salmonella
detected for a site or sample type did not correlate closely with the prevalence of positive
The sensitive detection and quantification of Salmonella in the flock
environment is practicable with the technique described. Quantitative data in many cases
does not correlate with qualitative findings.
Significance and Impact of Study: The significance of certain environmental factors and
interventions in the maintenance and dissemination of Salmonella in poultry houses may be
over- or under- represented by prevalence data alone. The technique described allows the
issue of poultry house contamination to be examined from a new perspective.
thermophilic Campylobacter spp. on finisher pig farms, Epidemiology & Infection 137 (8) pp. 1121-1134 Cambridge University Press
association of risk factors with the fluoroquinolone (FQ)-resistance status of 108 pig finisher farms
in Great Britain. The farms were classified as ?affected? or ?not affected? by FQ-resistant E. coli or
Campylobacter spp. on the basis of isolation of organisms from faecal samples on media containing
1 mg.l-1 FQ. The use of FQ was the most important factor associated with finding resistant E. coli
and/or Campylobacter, which were found on 79% (FQ-resistant E. coli) and 86% (FQ-resistant
Campylobacter) of farms with a history of FQ use. However, resistant bacteria were also found on
19% (FQ-resistant E. coli) and 54% (FQ-resistant Campylobacter) of farms with no history of FQ
use. For FQ-resistant E. coli, biosecurity measures may be protective and there was strong seasonal
variation, with more farms found affected when sampled in the summer. For FQ-resistant
Campylobacter, the buying in of grower stock may increase risk and good on-farm hygiene may be
protective. The findings suggest that resistant organisms, particularly Campylobacter, may spread
between pig farms.
sampled intensively for environmental Salmonella contamination. Eight pig breeding units
previously associated with Salmonella Typhimurium were visited during up to seven years. Samples
from voided faeces, surfaces, fomites and wildlife were cultured. Certain serovars were isolated
repeatedly on certain units, whilst others were detected only once or intermittently. A few serovars
were isolated consistently on some units but only intermittently on others. There was an association
between Salmonella in pens and in their immediate environment. S. Typhimurium was significantly
associated with growing pigs. Pens holding breeding stock for production herds were frequently
Salmonella-positive. Herds under common ownership showed similar serovar combinations.
Cleaning and disinfection was frequently ineffective. Wildlife serovars were typical of the associated
premises. On one unit, a low level of Salmonella was attributed to a small herd size, good cleaning
and disinfection and good rodent control. The study has shown that breeding herds are susceptible to
endemic infections with multiple Salmonella serovars and that cleaning, disinfection and vector
control may in many cases be inadequate. Finally, the prevalence of S. Typhimurium may be greater
in youngstock, which has important implications for public health.
poultry layer houses using qualitative and
semi-quantitative culture techniques, Veterinary Microbiology 116 (4) pp. 283-293 Elsevier
contamination of layer houses and associated wildlife vectors by S. Enteritidis. Methods for its
control and elimination include effective cleaning and disinfection of layer houses between flocks,
and it is important to be able to measure the success of such decontamination. A method for the
environmental detection and semi-quantitative enumeration of salmonellae was used and compared
with a standard qualitative method, in twelve Salmonella-contaminated caged layer houses before
and after cleaning and disinfection. The quantitative technique proved to have comparable sensitivity
to the standard method, and additionally provided insights into the numerical Salmonella challenge
that replacement flocks would encounter. Elimination of S. Enteritidis was not achieved in any of the
premises examined although substantial reductions in the prevalence and numbers of salmonellae
were demonstrated, while in others an increase in contamination was observed after cleaning and
disinfection. Particular problems with feeders and wildlife vectors were highlighted. The use of a
quantitative method assisted the identification of problem areas, such as those with a high initial
bacterial load or those experiencing only a modest reduction in bacterial count following
thermophilic Campylobacter spp. on poultry and pig farms in
Great Britain, Journal of Applied Microbiology 105 (5) pp. 1421-1431 Wiley
To estimate the proportions of farms on which broilers, turkeys and pigs were shedding
fluoroquinolone (FQ)-resistant E. coli or Campylobacter spp. near to slaughter.
Methods and Results:
Freshly-voided faeces were collected on 89 poultry and 108 pig farms and
cultured with media containing 1.0 mg l-1 ciprofloxacin. Studies demonstrated the specificity of this
sensitive method, and both poultry and pig sampling yielded FQ-resistant E. coli on 60% of farms.
FQ-resistant Campylobacter spp. were found on around 22% of poultry and 75% of pig farms. The
majority of resistant isolates of Campylobacter (89%) and E. coli (96%) tested had minimum
inhibitory concentrations for ciprofloxacin of e8 mg l-1. The proportion of resistant E. coli and
Campylobacter organisms within samples varied widely.
FQ resistance is commonly present among two enteric bacterial genera prevalent on
pig and poultry farms, although the low proportion of resistant organisms in many cases requires a
sensitive detection technique.
Significance and Impact of Study:
FQ-resistant bacteria with zoonotic potential appear to be
present on a high proportion of UK pig and poultry farms. The risk this poses to consumers relative
to other causes of FQ-resistant human infections remains to be clarified.
ruminants, FEMS Microbiology Reviews 33 (2) pp. 394-410 Oxford University Press
disease in the early 1980s, with ruminants cited as the primary reservoirs. Preliminary
studies indicated cattle to be the sole source of E. coli O157:H7 outbreaks in humans,
however further epidemiological studies soon demonstrated that E. coli O157:H7 was
widespread in other food sources and that a number of transmission routes existed. More
recently, small domestic ruminants (sheep and goats) have emerged as important sources
of E. coli O157:H7 human infection, particularly with the widespread popularity of petting
farms and the increased use of sheep and goat food products, including unpasteurised
cheeses. Although the colonisation and persistence characteristics of E. coli O157:H7 in the
bovine host have been studied intensively, this is not the case for small ruminants. Despite
many similarities with the bovine host, the pathobiology of E. coli O157:H7 in small
domestic ruminants does appear to differ significantly from that described in cattle. This
review aims to critically review the current knowledge regarding colonisation and
persistence of E. coli O157:H7 in small domestic ruminants, including comparisons with the
bovine host where appropriate.
contamination in caged and free-range layer flocks, Avian Pathology 36 (3) pp. 187-197 Taylor & Francis:
commercial layer flocks from eight farms in the UK, which previously had been identified as
being contaminated with salmonella. Samples of faeces, dust, litter, egg belt spillage and
wildlife vectors were taken, plus swabs of cages, feeders, drinkers, floors, egg belts and boots.
Some sampling was performed in each month of the year. Numerous serovars were detected
but Salmonella Enteritidis was the only persistent serotype found among single-age flocks.
There was a significant correlation between qualitative environmental samples and semiquantitative
faeces samples. The level of environmental contamination increased significantly
over time. There were significant temperature and seasonal effects upon contamination.
Wildlife vectors proved to be sensitive samples for the detection of salmonella. The efficacy
of cleaning and disinfection upon residual salmonella contamination, and upon subsequent
flock contamination, was highly variable between and within premises.
The variability between detected prevalences over time and between flocks indicates a
need for regular, sensitive monitoring of flocks for salmonella to permit targeting of control
measures aimed at eliminating contamination of the layer environment by salmonella. There
is substantial scope for improvement of cleaning and disinfection procedures.
Salmonella, FOODBORNE PATHOGENS AND DISEASE 7 (1) pp. 3-15 MARY ANN LIEBERT INC
food chain from contamination by Salmonella derived from infected animals. The
transmission of Salmonella from animal feeds to animals, and onwards to human food
products, has been convincingly documented. This is especially important for chicken
breeding and laying flocks and pigs, in view of the consequences of recent or imminent
control legislation in the European Union. Animal feed ingredients, particularly animal and
plant-derived protein meals, are frequently contaminated with Salmonella either from source
or from processing plant, and recontamination in compounding mills is an additional problem.
Several complementary strategies have been used to control this feed contamination, and
these include a range of chemical treatments. The principal agents used are: organic acids and
their salts, formaldehyde, and bacterial membrane disruptors such as terpenes and essential
oils. Experimental agents include chlorate compounds. Many products use blends of agents
from the same or different chemical groups to achieve synergistic or combination effects. The
present review draws upon published and company data to describe the various modes of
action and efficacies of different chemical agents delivered in feed or in drinking water
against Salmonella occurring in feed or in livestock environments. Reasons for the failure of
protection are explored, along with problems in usage such as corrosion and reduced
palatability. Given the wide array of products available with contrasting modes of action, the
need for standardised tests of efficacy is also discussed.
Biosecurity and the Potential Role of Disinfectants in
Resistance Selection, Comprehensive Reviews in Food Science and Food Safety 18 (3) pp. 753-774 Wiley
both human and veterinary medicine, and the need to address the issue in both of these linked
domains is a current priority in public policy. Efforts to limit antimicrobial resistance on farms has so
far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to
reduce infectious disease. In the United Kingdom (UK) and some other countries, substantial
progress has been made recently against targets on agricultural antimicrobial drug use. However,
evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within
and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely
complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated
with maintenance and replication of resistance genes and associated proteins, horizontal
transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via
movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and coselection
of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides.
Areas of particular concern for public health include extended-spectrum cephalosporinases and
fluoroquinolone resistance among Enterobacteriaceae, livestock-associated methicillin-resistant
Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of
biosecurity have repeatedly been identified as risk factors for the presence of antimicrobial
resistance on farm premises, but there are large gaps in our understanding of the most important
risk factors and the most effective interventions. The present review aims to summarize the present
state of knowledge in this area, from a European perspective.
Following a rapid rise in cases of monophasic Salmonella Typhimurium DT193 (mST) in humans and pigs since 2007 a detailed study of the prevalence and persistence of mST on pig and cattle farms in Great Britain (GB) was undertaken. Thirteen commercial pig farms and twelve cattle farms, identified as mST-positive from surveillance data, were intensively sampled over a three year period. Five indoor and eight outdoor pig farms and four beef and eight dairy farms were included. Individual and pooled faecal samples were collected from each epidemiological group and environmental samples throughout each farm and the antimicrobial resistance profile
determined for a selection of mST-positive isolates.
Indoor pig farms had a higher mST prevalence than outdoor pig farms, and across both cattle and pig farms the juvenile animals had a higher mST prevalence than the adult animals. Overall, mST prevalence decreased with time across all pig farms, from 25% to less than 15% of environmental samples and 22% to 15% of pooled faecal samples; only one organic outdoor breeding farm was Salmonella-negative at the end of the study. Across the cattle farms no mST was detected by the end of the study, apart from one persistent farm. Clearance time of
mST was between seven and twenty-five months. Farms were selected based on having the antimicrobial resistance profile ampicillin, streptomycin, sulphonamides and tetracycline (A, S, SU, T), although resistance to trimethoprim-potentiated sulphamethoxazole was also identified on five pig farms sampled.
This study provided a detailed insight into the distribution and persistence of mST on individual pig and cattle farms in GB. It has identified variation in mST shedding of individual animals, and the data can be applied to the wider livestock industry when considering the distribution of mST once identified on an individual farm.