Professor Simon N Archer

Professor Simon N Archer FRSB


Professor of Molecular Biology of Sleep
+44 (0)1483 686408

About

University roles and responsibilities

  • Member University Ethics Committee (2020-)
  • Head Department of Biochemical Sciences (2014-2017)
  • Member University Ethical Review Committee (2009-2012)
  • Member Faculty Research & Enterprise Committee (2007-2012)
  • Sleep, Chronobiology & Neuroscience Theme Leader (2007-2012)
  • Biochemistry Admissions Tutor (2006-2016)
  • Level 4 Exams Officer (2005-2006)
  • Chair Faculty Seminar & Research Festival Committee (2004-2008)

My qualifications

1985
BSc Biology
University of Sussex
1989
PhD Zoology
University of Bristol
2016
Fellow Royal Society of Biology

Affiliations and memberships

European Sleep Research Society
Member
Scientific Committee Member (2010-2014)
Scientific Committee Chair (2012-2014)
Research Networks Committee Member (2010-2014)
Society for Research on Biological Rhythms
Member
Trainee Research Awards Committee Member (2011-2014)
European Biological Rhythms Society
Member

Research

Research interests

Indicators of esteem

  • Editorial Board Member, Frontiers in Neuroscience - Sleep & Circadian Rhythms

  • Editorial Board Member, Clocks & Sleep

  • Member Royal Society Research Grants Panel (2014-2020)

  • Member Canadian Institutes of Health Research Grant Review Panel (2016/2017)

  • Member UKRI Future Leader Fellowships Peer Review College (2018-)

  • Member ERC Expert Review Pool

Publications

Michelle Magee, Tracey L. Sletten, Jade M. Murray, Christopher J. Gordon, Nicole Lovato, Delwyn J. Bartlett, David J. Kennaway, Steven W. Lockley, Leon C. Lack, Ronald R. Grunstein, Simon Archer, Shantha M.W Rajaratnam (2020)A PERIOD3 variable-number-tandem-repeat polymorphism modulates melatonin treatment response in Delayed Sleep-Wake Phase Disorder, In: Journal of Pineal Research69(4)pp. e12684-n/a Wiley

We examined whether a polymorphism of the PERIOD3 gene (PER3; rs57875989) modulated the sleep promoting effects of melatonin in Delayed Sleep-Wake Phase Disorder (DSWPD). One hundred and four individuals (53 males; 29.4±10.0 years) with DSWPD and a delayed dim light melatonin onset (DLMO) collected buccal swabs for genotyping (PER34/4 n=43; PER3 5 allele [heterozygous and homozygous] n=60). Participants were randomised to placebo or 0.5mg melatonin taken 1 hour before desired bedtime (or ~ 1.45 h before DLMO), with sleep attempted at desired bedtime (4 weeks; 5-7 nights/week). We assessed sleep (diary and actigraphy), Pittsburgh Sleep Quality Index (PSQI), Insomnia Severity Index (ISI), Patient-Reported Outcomes Measurement Information System (PROMIS: Sleep Disturbance, Sleep-Related Impairment), Sheehan Disability Scale (SDS), and Patient- and Clinician-Global Improvement (PGI-C, CGI-C). Melatonin treatment response on actigraphic sleep onset time did not differ between genotypes. For PER34/4 carriers, self-reported sleep onset time was advanced by a larger amount and sleep onset latency (SOL) was shorter in melatonin-treated patients compared to those receiving placebo (P=0.008), while actigraphic sleep efficiency in the first third of the sleep episode (SE T1) did not differ. For PER3 5 carriers, actigraphic SOL and SE T1 showed a larger improvement with melatonin (P

Daan R. van der Veen, Emma Laing, Sung-Eun Bae, Jonathan Johnston, Derk-Jan Dijk, Simon Archer (2020)A Topological Cluster of Differentially Regulated Genes in Mice Lacking PER3, In: Frontiers in Molecular Neuroscience13(15) Frontiers Media

Polymorphisms in the human circadian clock gene PERIOD3 (PER3) are associated with a wide variety of phenotypes such as diurnal preference, delayed sleep phase disorder, sleep homeostasis, cognitive performance, bipolar disorder, type 2 diabetes, cardiac regulation, cancer, light sensitivity, hormone and cytokine secretion, and addiction. However, the molecular mechanisms underlying these phenotypic associations remain unknown. Per3 knockout mice (Per3

Emma E. Laing, Carla S. Moller-Levet, Derk-Jan Dijkand, Simon N. Archer (2020)Identifying and validating blood mRNA biomarkers for acute and chronic insufficient sleep in humans: a machine learning approach (vol 42, zsy186, 2019), In: Sleep (New York, N.Y.)43(6)313 Oxford Univ Press
Carla S. Möller-Levet, Emma E. Laing, Simon N. Archer, Derk-Jan Dijk (2022)Diurnal and circadian rhythmicity of the human blood transcriptome overlaps with organ and tissue specific expression of a non-human primate, In: BMC Biology BMC

BACKGROUND: Twenty-four-hour rhythmicity in mammalian tissues and organs is driven by local circadian oscillators, systemic factors, the central circadian pacemaker, and light-dark cycles. At the physiological level, the neural and endocrine systems synchronize gene expression in peripheral tissues and organs to the twenty-four-hour day cycle, and disruption of such regulation has been shown to lead to pathological conditions. Thus, monitoring rhythmicity in tissues/organs holds promise for circadian medicine, however most tissues and organs are not easily accessible in humans and alternative approaches to quantify circadian rhythmicity are needed. We investigated the overlap between rhythmic transcripts in human blood and transcripts shown to be rhythmic in 64 tissues/organs of the baboon, how these rhythms are aligned with light-dark cycles and each other, and whether timing of tissue-specific rhythmicity can be predicted from a blood sample. RESULTS: We compared rhythmicity in transcriptomic time series collected from humans and baboons using set logic, circular cross-correlation, circular clustering, functional enrichment analyses and least squares regression. Of the 759 orthologous genes that were rhythmic in human blood, 652 (86%) were also rhythmic in at least one baboon tissue and most of these genes were associated with basic processes such as transcription and protein homeostasis. 109 (17%) of the 652 overlapping rhythmic genes were reported as rhythmic in only one baboon tissue or organ and several of these genes have tissue/organ-specific functions. The timing of human and baboon rhythmic transcripts displayed prominent ‘night’ and ‘day’ clusters, with genes in the dark cluster associated with translation. Alignment between baboon rhythmic transcriptomes and the overlapping human blood transcriptome was significantly closer when light onset, rather than midpoint of light, or end of light period, was used as phase reference point. The timing of overlapping human and baboon rhythmic transcriptomes was significantly correlated in 25 tissue/organs with an average earlier timing of 3.21 h (SD 2.47 h) in human blood. CONCLUSIONS: The human blood transcriptome contains sets of rhythmic genes that overlap with rhythmic genes of tissues/organs in baboon. The rhythmic sets vary across tissues/organs, but the timing of most rhythmic genes is similar in human blood and baboon tissues/organs. These results have implications for development of blood transcriptome-based biomarkers for circadian rhythmicity in tissues and organs.

© Cambridge University Press 2013.It would be extremely unusual, not to mention highly inconvenient, if everyone woke up and went about their daily routines at the same time. Fortunately this is not the case, and humans display a wide range of sleep–wake timing preferences. Some of us like to wake up and get things done in the morning (so-called larks, or morning types), others prefer to be active later in the day and night (owls, or evening types), and many are in between or a mixture of the two. The range in sleep–wake timing is considerable and differences in preferred bedtime and wake time can be as much as 2–3 The on average between morning and evening types [1], and in circadian rhythm sleep phase disorders, bedtimes can range from 7–9 p.m. (advanced) to 2–6 a.m. (delayed) [2]. It has often been assumed that diurnal preference (morningness versus eveningness) is not an acquired characteristic but relates to biological factors involved in the circadian timing system that regulates the optimum times for waking performance and sleep–wake timing. However, current understanding of factors influencing variation in sleep–wake timing and optimal timing of waking performance emphasizes the interactive contribution of social factors, such as work schedules and leisure time, and biological factors. Underlying biological factors include the timing (phase of entrainment) of the endogenous circadian rhythmicity relative to clock time, and the light–dark cycle [3]. The phase of entrainment is determined by the intrinsic period of the circadian clock, as well as sensitivity to the effects of light on the circadian clock. In addition, sleep homeostatic mechanisms also play an important role in sleep–wake timing. This implies that diurnal preference could be related to any of these three main factors: circadian period, light sensitivity, and sleep homeostasis.

Skevoulla Christou, Sophie M T Wehrens, Cheryl Isherwood, Carla S Moller-Levet, Huihai Wu, Victoria L Revell, Giselda Bucca, Debra J Skene, Emma E Laing, Simon N Archer, Jonathan D Johnston (2019)Circadian regulation in human white adipose tissue revealed by transcriptome and metabolic network analysis, In: Scientific Reports Nature Research

Studying circadian rhythms in most human tissues is hampered by difficulty in collecting serial samples. Here we reveal circadian rhythms in the transcriptome and metabolic pathways of human white adipose tissue. Subcutaneous adipose tissue was taken from seven healthy males under highly controlled ‘constant routine’ conditions. Five biopsies per participant were taken at six-hourly intervals for microarray analysis and in silico integrative metabolic modelling. We identified 837 transcripts exhibiting circadian expression profiles (2% of 41619 transcript targeting probes on the array), with clear separation of transcripts peaking in the morning (258 probes) and evening (579 probes). There was only partial overlap of our rhythmic transcripts with published animal adipose and human blood transcriptome data. Morning-peaking transcripts associated with regulation of gene expression, nitrogen compound metabolism, and nucleic acid biology; evening-peaking transcripts associated with organic acid metabolism, cofactor metabolism and redox activity. In silico pathway analysis further indicated circadian regulation of lipid and nucleic acid metabolism; it also predicted circadian variation in key metabolic pathways such as the citric acid cycle and branched chain amino acid degradation. In summary, in vivo circadian rhythms exist in multiple adipose metabolic pathways, including those involved in lipid metabolism, and core aspects of cellular biochemistry.

© Cambridge University Press 2013.In humans, the sleep–wake cycle is determined by the interaction of the endogenous circadian clock and sleep homeostat, and exogenous factors such as the light/dark cycle, which is important for circadian entrainment, and social influences such as work and recreation (Figure 31.1). These factors interact and it is often difficult to determine the causes and nature of altered sleep–wake timing. Abnormal sleep–wake timing may be a simple consequence of an abnormal phase relationship of the circadian clock and environmental time. This may be caused by aberrant light exposure patterns or extreme intrinsic periods of the circadian clock. The timing of the sleep–wake cycle relative to the circadian sleep propensity rhythm may be altered because of fast or slow build-up of homeostatic sleep pressure. Recent mathematical models of the sleep–wake cycle have indeed demonstrated that one particular phenotype may be related to parameters of very different processes [1]. Here, we focus on some of the genetic factors that are associated with abnormally delayed sleep timing, and explore to what extent the effects of these factors can be attributed to physiological processes such as light sensitivity, sleep homeostasis or circadian period. Circadian rhythm sleep disorders (CRSDs) refer to sleep disturbances that are primarily due to alterations of the circadian time-keeping system or are related to a misalignment of endogenous circadian rhythms and the required sleep–wake time (see [2]). The latter distinction is important because social factors may necessitate a non-desirable sleep–wake schedule, as occurs in shift work, for example. Shift work disorder and jet lag disorder are CRSDs that are caused by exogenous factors, whereas dysfunction of the endogenous circadian clock is thought to be the primary cause of delayed sleep phase disorder (DSPD). A better understanding of what causes CRSDs and inter-individual vulnerability differences is important because of the large proportion of the population who regularly undertake shift work, the epidemiological evidence linking insufficient sleep with negative health outcomes [3], and known associations between extreme evening preference and health problems such as mood disorders, metabolic disorders, and cardiovascular risk (see [4]).

Vincenzo Muto, Ekaterina Koshmanova, Pouya Ghaemmaghami, Mathieu Jaspar, Christelle Meyer, Mahmoud Elansary, Maxime Van Egroo, Daphne Chylinski, Christina Berthomier, Marie Brandewinder, Charlotte Mouraux, Christina Schmidt, Gregory Hammad, Wouter Coppieters, Naima Ahariz, Christian Degueldre, Andre Luxen, Eric Salmon, Christophe Phillips, Simon N. Archer, Loic Yengo, Enda Byrne, Fabienne Collette, Michel Georges, Derk-Jan Dijk, Pierre Maquet, Peter M. Visscher, Gilles Vandewalle (2020)Alzheimer?s disease genetic risk and sleep phenotypes: association with more slow-waves and daytime sleepiness, In: Sleep Oxford University Press

Study Objectives. Sleep disturbances and genetic variants have been identified as risk factors for Alzheimer’s disease. Our goal was to assess whether genome-wide polygenic risk scores (PRS) for AD associate with sleep phenotypes in young adults, decades before typical AD symptom onset. Methods. We computed whole-genome Polygenic Risk Scores (PRS) for AD and extensively phenotyped sleep under different sleep conditions, including baseline sleep, recovery sleep following sleep deprivation and extended sleep opportunity, in a carefully selected homogenous sample of healthy 363 young men (22.1 y ± 2.7) devoid of sleep and cognitive disorders. Results. AD PRS was associated with more slow wave energy, i.e. the cumulated power in the 0.5-4 Hz EEG band, a marker of sleep need, during habitual sleep and following sleep loss, and potentially with large slow wave sleep rebound following sleep deprivation. Furthermore, higher AD PRS was correlated with higher habitual daytime sleepiness. Conclusions. These results imply that sleep features may be associated with AD liability in young adults, when current AD biomarkers are typically negative, and the notion that quantifying sleep alterations may be useful in assessing the risk for developing AD.

SN Archer, P Ahuja, R Caffe, C Mikol, RG Foster, T van Veen, M von Schantz (2004)Absence of phosphoglucose isomerase-1 in retinal photoreceptor, pigment epithelium and Muller cells, In: EUROPEAN JOURNAL OF NEUROSCIENCE19(11)pp. 2923-2930 BLACKWELL PUBLISHING LTD
JC PARTRIDGE, J SHAND, SN ARCHER, JN LYTHGOE, WAHM VANGRONINGENLUYBEN (1989)INTERSPECIFIC VARIATION IN THE VISUAL PIGMENTS OF DEEP-SEA FISHES, In: JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY164(4)pp. 513-529 SPRINGER VERLAG
SN ARCHER, JN LYTHGOE, L HALL (1992)ROD OPSIN CDNA SEQUENCE FROM THE SAND GOBY (POMATOSCHISTUS-MINUTUS) COMPARED WITH THOSE OF OTHER VERTEBRATES, In: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES248(1321)pp. 19-25 ROYAL SOC
SN Archer, EE Laing, Carla S. Moller-Levet, Daan R. van der Veen, G Bucca, AS Lazar, N Santhi, A Slak, R Kabiljo, M von Schantz, CP Smith, DJ Dijk (2014)Mistimed sleep disrupts circadian regulation of the human transcriptome, In: Proceedings of the National Academy of Sciences (PNAS) National Academy of Sciences

Circadian organization of the mammalian transcriptome is achieved by rhythmic recruitment of key modifiers of chromatin structure and transcriptional and translational processes. These rhythmic processes, together with posttranslational modification, constitute circadian oscillators in the brain and peripheral tissues, which drive rhythms in physiology and behavior, including the sleep-wake cycle. In humans, sleep is normally timed to occur during the biological night, when body temperature is low and melatonin is synthesized. Desynchrony of sleep-wake timing and other circadian rhythms, such as occurs in shift work and jet lag, is associated with disruption of rhythmicity in physiology and endocrinology. However, to what extent mistimed sleep affects the molecular regulators of circadian rhythmicity remains to be established. Here, we show that mistimed sleep leads to a reduction of rhythmic transcripts in the human blood transcriptome from 6.4% at baseline to 1.0% during forced desynchrony of sleep and centrally driven circadian rhythms. Transcripts affected are key regulators of gene expression, including those associated with chromatin modification (methylases and acetylases), transcription (RNA polymerase II), translation (ribosomal proteins, initiation, and elongation factors), temperature-regulated transcription (cold inducible RNA-binding proteins), and core clock genes including CLOCK and ARNTL (BMAL1). We also estimated the separate contribution of sleep and circadian rhythmicity and found that the sleep-wake cycle coordinates the timing of transcription and translation in particular. The data show that mistimed sleep affects molecular processes at the core of circadian rhythm generation and imply that appropriate timing of sleep contributes significantly to the overall temporal organization of the human transcriptome.

JC Lo, JA Groeger, N Santhi, EL Arbon, AS Lazar, S Hasan, M von Schantz, SN Archer, DJ Dijk (2012)Effects of partial and acute total sleep deprivation on performance across cognitive domains, individuals and circadian phase., In: PLoS One7(9)pp. e45987-?

Cognitive performance deteriorates during extended wakefulness and circadian phase misalignment, and some individuals are more affected than others. Whether performance is affected similarly across cognitive domains, or whether cognitive processes involving Executive Functions are more sensitive to sleep and circadian misalignment than Alertness and Sustained Attention, is a matter of debate.

JQM Ly, G Gaggioni, SL Chellappa, S Papachilleos, A Brzozowski, C Borsu, M Rosanova, S Sarasso, Benita Middleton, A Luxen, Simon Archer, C Phillips, Derk-Jan Dijk, P Maquet, M Massimini, G Vandewalle (2016)Circadian regulation of human cortical excitability, In: Nature Communications11828 Nature Publishing Group

Prolonged wakefulness alters cortical excitability, which is essential for proper brain function and cognition. However, besides prior wakefulness, brain function and cognition are also affected by circadian rhythmicity. Whether the regulation of cognition involves a circadian impact on cortical excitability is unknown. Here, we assessed cortical excitability from scalp EEG-responses to transcranial magnetic stimulation in 22 participants during 29-h of wakefulness under constant conditions. Data reveal robust circadian dynamics of cortical excitability that were strongest in those individuals with highest endocrine markers of circadian amplitude. In addition, the time course of cortical excitability correlated with changes in EEG synchronization and cognitive performance. These results demonstrate that the crucial factor for cortical excitability, and basic brain function in general, is the balance between circadian rhythmicity and sleep need, rather than sleep homeostasis alone. These findings have implications for clinical applications such as noninvasive brain stimulation in neurorehabilitation.

SN ARCHER, JA ENDLER, JN LYTHGOE, JC PARTRIDGE (1987)VISUAL PIGMENT POLYMORPHISM IN THE GUPPY POECILIA-RETICULATA, In: VISION RESEARCH27(8)pp. 1243-1252 PERGAMON-ELSEVIER SCIENCE LTD
A Lazar, N Santhi, S Hasan, JCY Lo, JD Johnston, M von Schantz, SN Archer, DJ Dijk (2017)Intrinsic Circadian Period and the Melatonin Rhythm in Men and Women: Predictors of Sleep during the Weekend and in the Laboratory, In: Journal of Sleep Research22(2)pp. 155-159

Sleep complaints and irregular sleep patterns, such as curtailed sleep during workdays and longer and later sleep during weekends are common. It is often implied that differences in circadian period and in entrained phase contribute to these patterns but few data are available. We assessed parameters of the circadian rhythm of melatonin at baseline and in a forced desynchrony protocol in 35 participants (18 women) with no sleep disorders. Intrinsic circadian period varied between 23h50min and 24h31min and correlated positively (n=31, rs=0.43, P=0.017) with the timing of the melatonin rhythm relative to habitual bedtime. This phase of the melatonin rhythm correlated with the insomnia severity score (n=35, rs=0.47, P=0.004). Self-reported time in bed (TIB) during free days also correlated with the timing of the melatonin rhythm (n=35, rs=0.43, P=0.01) as well as with circadian period (n=31, rs=0.47, P=0.007) such that individuals with a more delayed melatonin rhythm or a longer circadian period reported longer sleep during the weekend. The increase in TIB during the free days correlated positively with circadian period (n=31, rs=0.54, P=0.002). Polysomnographically-assessed latency to persistent sleep (n=34, rs=0.48, P=0.004) correlated with the timing of the melatonin rhythm when participants were sleeping at their habitual bedtimes in the laboratory. This correlation was significantly stronger in women than in men (Z=2.38, P=0.017). The findings show that individual differences in period and phase of the circadian melatonin rhythm associate with differences in sleep and imply that individuals with a long circadian period are at risk of developing sleep problems.

SN ARCHER, JN LYTHGOE (1990)THE VISUAL PIGMENT BASIS FOR CONE POLYMORPHISM IN THE GUPPY, POECILIA-RETICULATA, In: VISION RESEARCH30(2)pp. 225-233 PERGAMON-ELSEVIER SCIENCE LTD
AU Viola, SN Archer, LM James, JA Groeger, JCY Lo, DJ Skene, M von Schantz, D-J Dijk (2007)PER3 polymorphism predicts sleep structure and waking performance, In: CURRENT BIOLOGY17(7)pp. 613-618 CELL PRESS
AU Viola, LM James, SN Archer, D-J Dijk (2008)PER3 polymorphism and cardiac autonomic control: effects of sleep debt and circadian phase, In: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY295(5)pp. H2156-H2163 AMER PHYSIOLOGICAL SOC
A Jenkins, SN Archer, M von Schantz (2005)Expansion during primate radiation of a variable number tandem repeat in the coding region of the circadian clock gene Period3, In: JOURNAL OF BIOLOGICAL RHYTHMS20(5)pp. 470-472 SAGE PUBLICATIONS LTD
NL Barclay, TC Eley, DJ Buysse, SN Archer, AM Gregory (2010)Diurnal preference and sleep quality: Same genes? A study of young adult twins, In: Chronobiology International27(2)pp. 278-296 Infoma Healthcare
Sophie Wehrens, Skevoulla Christou, Cheryl Isherwood, Benita Middleton, Michelle Gibbs, Simon Archer, Debra Skene, Jonathan Johnston (2017)Meal Timing Regulates the Human Circadian System, In: Current Biology27(12)pp. 1768-1775e3 Elsevier (Cell Press)

Circadian rhythms, metabolism and nutrition are intimately linked [1, 2], although effects of meal timing on the human circadian system are poorly understood. We investigated the effect of a 5-hour delay in meals on markers of the human master clock and multiple peripheral circadian rhythms. Ten healthy young men undertook a 13-day laboratory protocol. Three meals (breakfast, lunch, dinner) were given at 5-hour intervals, beginning either 0.5 (early) or 5.5 (late) hours after wake. Participants were acclimated to early meals and then switched to late meals for 6 days. After each meal schedule, participants' circadian rhythms were measured in a 37-hour constant routine that removes sleep and environmental rhythms while replacing meals with hourly isocaloric snacks. Meal timing did not alter actigraphic sleep parameters before circadian rhythm measurement. In constant routines, meal timing did not affect rhythms of subjective hunger and sleepiness, master clock markers (plasma melatonin and cortisol), plasma triglycerides, or clock gene expression in whole blood. Following late meals, however, plasma glucose rhythms were delayed by 5.69 ± 1.29 hours (p < 0.001) and average glucose concentration decreased by 0.27 ± 0.05 mM (p < 0.001). In adipose tissue, PER2 mRNA rhythms were delayed by 0.97 ± 0.29 hours (p < 0.01), indicating that human molecular clocks may be regulated by feeding time and could underpin plasma glucose changes. Timed meals therefore play a role in synchronising peripheral circadian rhythms in humans, and may have particular relevance for patients with circadian rhythm disorders, shift workers, and transmeridian travellers.

AS Lazar, N Santhi, S Hasan, JC-Y Lo, JD Johnston, MV Schantz, SN Archer, D-J Dijk (2013)Circadian period and the timing of melatonin onset in men and women: Predictors of sleep during the weekend and in the laboratory, In: Journal of Sleep Research22(2)pp. 155-159

Sleep complaints and irregular sleep patterns, such as curtailed sleep during workdays and longer and later sleep during weekends, are common. It is often implied that differences in circadian period and in entrained phase contribute to these patterns, but few data are available. We assessed parameters of the circadian rhythm of melatonin at baseline and in a forced desynchrony protocol in 35 participants (18 women) with no sleep disorders. Circadian period varied between 23 h 50 min and 24 h 31 min, and correlated positively (n = 31, rs = 0.43, P = 0.017) with the timing of the melatonin rhythm relative to habitual bedtime. The phase of the melatonin rhythm correlated with the Insomnia Severity Index (n = 35, rs = 0.47, P = 0.004). Self-reported time in bed during free days also correlated with the timing of the melatonin rhythm (n = 35, rs = 0.43, P = 0.01) as well as with the circadian period (n = 31, rs = 0.47, P = 0.007), such that individuals with a more delayed melatonin rhythm or a longer circadian period reported longer sleep during the weekend. The increase in time in bed during the free days correlated positively with circadian period (n = 31, rs = 0.54, P = 0.002). Polysomnographically assessed latency to persistent sleep (n = 34, rs = 0.48, P = 0.004) correlated with the timing of the melatonin rhythm when participants were sleeping at their habitual bedtimes in the laboratory. This correlation was significantly stronger in women than in men (Z = 2.38, P = 0.017). The findings show that individual differences in circadian period and phase of the melatonin rhythm associate with differences in sleep, and suggest that individuals with a long circadian period may be at risk of developing sleep problems.

M von Schantz, SN Archer (2003)Clocks, genes and sleep, In: JOURNAL OF THE ROYAL SOCIETY OF MEDICINE96(10)pp. 486-489 ROYAL SOC MEDICINE PRESS LTD
JD Carpen, M von Schantz, M Smits, DJ Skene, SN Archer (2006)A silent polymorphism in the PER1 gene associates with extreme diurnal preference in humans, In: JOURNAL OF HUMAN GENETICS51(12)pp. 1122-1125 SPRINGER TOKYO
Emma Laing, Jonathan Johnston, Carla Moller-Levet, G Bucca, CP Smith, Derk-Jan Dijk, Simon Archer (2015)Exploiting human and mouse transcriptomic data: Identification of circadian genes and pathways influencing health, In: BioEssays37(5)pp. 544-556 Wiley

The power of the application of bioinformatics across multiple publicly available transcriptomic data sets was explored. Using 19 human and mouse circadian transcriptomic data sets, we found that NR1D1 and NR1D2 which encode heme-responsive nuclear receptors are the most rhythmic transcripts across sleep conditions and tissues suggesting that they are at the core of circadian rhythm generation. Analyzes of human transcriptomic data show that a core set of transcripts related to processes including immune function, glucocorticoid signalling, and lipid metabolism is rhythmically expressed independently of the sleep-wake cycle. We also identify key transcripts associated with transcription and translation that are disrupted by sleep manipulations, and through network analysis identify putative mechanisms underlying the adverse health outcomes associated with sleep disruption, such as diabetes and cancer. Comparative bioinformatics applied to existing and future data sets will be a powerful tool for the identification of core circadian- and sleep-dependent molecules.

S Archer, J Hirano (1996)Absorbance spectra and molecular structure of the blue-sensitive rod visual pigment in the conger eel (Conger conger), In: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES263(1371)pp. 761-767 ROYAL SOC
AL Hogben, J Ellis, SN Archer, M von Schantzi (2007)Conscientiousness is a predictor of diurnal preference, In: CHRONOBIOLOGY INTERNATIONAL24(6)pp. 1249-1254 TAYLOR & FRANCIS INC
SN Archer, J Hirano (1997)Opsin sequences of the rod visual pigments in two species of Poeciliid fish, In: JOURNAL OF FISH BIOLOGY51(1)pp. 215-219 ACADEMIC PRESS LTD
A Adan, SN Archer, M Paz Hidalgo, L Di Milia, V Natale, C Randler (2012)Circadian Typology: A Comprehensive Review, In: CHRONOBIOLOGY INTERNATIONAL29(9)pp. 1153-1175 INFORMA HEALTHCARE
S Archer, A Hope, JC Partridge (1995)The molecular basis for the green-blue sensitivity shift in the rod visual pigments of the European eel, In: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES262(1365)pp. 289-295 ROYAL SOC
MJ Parsons, PM Nolan, KJ Lester, TC Eley, NL Barclay, SN Archer, AM Gregory (2014)Polymorphisms in the circadian expressed genes PER3 and ARNTL2 are associated with diurnal preference and GNβ3 with sleep measures, In: Journal of Sleep Research

Sleep and circadian rhythms are intrinsically linked, with several sleep traits, including sleep timing and duration, influenced by both sleep homeostasis and the circadian phase. Genetic variation in several circadian genes has been associated with diurnal preference (preference in timing of sleep), although there has been limited research on whether they are associated with other sleep measurements. We investigated whether these genetic variations were associated with diurnal preference (Morningness-Eveningness Questionnaire) and various sleep measures, including: the global Pittsburgh Sleep Quality index score; sleep duration; and sleep latency and sleep quality. We genotyped 10 polymorphisms in genes with circadian expression in participants from the G1219 sample (n = 966), a British longitudinal population sample of young adults. We conducted linear regressions using dominant, additive and recessive models of inheritance to test for associations between these polymorphisms and the sleep measures. We found a significant association between diurnal preference and a polymorphism in period homologue 3 (PER3) (P < 0.005, recessive model) and a novel nominally significant association between diurnal preference and a polymorphism in aryl hydrocarbon receptor nuclear translocator-like 2 (ARNTL2) (P < 0.05, additive model). We found that a polymorphism in guanine nucleotide binding protein beta 3 (GNβ3) was associated significantly with global sleep quality (P < 0.005, recessive model), and that a rare polymorphism in period homologue 2 (PER2) was associated significantly with both sleep duration and quality (P < 0.0005, recessive model). These findings suggest that genes with circadian expression may play a role in regulating both the circadian clock and sleep homeostasis, and highlight the importance of further studies aimed at dissecting the specific roles that circadian genes play in these two interrelated but unique behaviours. © 2014 The Authors.

JKJ Diss, SN Archer, J Hirano, SP Fraser, MBA Djamgoz (2001)Expression profiles of voltage-gated Na+ channel alpha-subunit genes in rat and human prostate cancer cell lines, In: PROSTATE48(3)pp. 165-178 WILEY-LISS
Emma Laing, Carla Moller-Levet, Derk-Jan Dijk, Simon Archer (2018)Identifying and validating blood mRNA biomarkers for acute and chronic insufficient sleep in humans: a machine learning approach, In: Supplemental material for "Identifying and validating blood mRNA biomarkers for acute and chronic insufficient sleep in humans: a machine learning approach" Oxford University Press

Acute and chronic insufficient sleep are associated with adverse health outcomes and risk of accidents. There is therefore a need for biomarkers to monitor sleep debt status. None are currently available. We applied Elastic-net and Ridge regression to entire and pre-filtered transcriptome samples collected in healthy young adults during acute total sleep deprivation and following 1 week of either chronic insufficient (< 6 h) or sufficient sleep (~8.6 h) to identify panels of mRNA biomarkers of sleep debt status. The size of identified panels ranged from 9-74 biomarkers. Panel performance, assessed by leave-one-subject-out cross-validation and independent validation, varied between sleep debt conditions. Using between-subject assessments based on one blood sample, the accuracy of classifying ‘Acute sleep loss’ was 92%, but only 57% for classifying ‘Chronic sleep insufficiency’. A reasonable accuracy for classifying ‘chronic sleep insufficiency’ could only be achieved by a within-subject comparison of blood samples. Biomarkers for sleep debt status showed little overlap with previously identified biomarkers for circadian phase. Biomarkers for acute and chronic sleep loss also showed little overlap but were associated with common functions related to the cellular stress response, such as heat shock protein activity, the unfolded protein response, protein ubiquitination and endoplasmic reticulum associated protein degradation, and apoptosis. This characteristic response of whole blood to sleep loss can further aid our understanding of how sleep insufficiencies negatively affect health. Further development of these novel biomarkers for research and clinical practice requires validation in other protocols and age groups.

N Santhi, JA Groeger, SN Archer, M Gimenez, LJ Schlangen, DJ Dijk (2013)Morning sleep inertia in alertness and performance: effect of cognitive domain and white light conditions., In: PLoS One8(11)pp. e79688-?

The transition from sleep to wakefulness entails a temporary period of reduced alertness and impaired performance known as sleep inertia. The extent to which its severity varies with task and cognitive processes remains unclear. We examined sleep inertia in alertness, attention, working memory and cognitive throughput with the Karolinska Sleepiness Scale (KSS), the Psychomotor Vigilance Task (PVT), n-back and add tasks, respectively. The tasks were administered 2 hours before bedtime and at regular intervals for four hours, starting immediately after awakening in the morning, in eleven participants, in a four-way cross-over laboratory design. We also investigated whether exposure to Blue-Enhanced or Bright Blue-Enhanced white light would reduce sleep inertia. Alertness and all cognitive processes were impaired immediately upon awakening (p

Sareh Panjeh, Sabine Pompeia, Simon Archer, Mario Pedrazzoli, Malcolm von Schantz, Hugo Cogo-Moreira (2020)What are we measuring with the Morningness-Eveningness Questionnaire? Exploratory factor analysis across four samples from two countries, In: Chronobiology International38(2)Taylor & Francis Taylor and Francis

Individual variability in diurnal preference or chronotype is commonly assessed with selfreport scales such as the widely used Morningness-Eveningness Questionnaire (MEQ). We sought to investigate the MEQ’s internal consistency by applying exploratory factor analysis (EFA) to determine the number of underlying latent factors in four different adult samples, two each from the United Kingdom and Brazil (total N=3,457). We focused on factors that were apparent in all samples, irrespective of particular sociocultural diversity and geographical characteristics, so as to show a common core reproducible structure across samples. Results showed a three-factor solution with acceptable to good model fit indexes in all studied populations. Twelve of the 19 MEQ items in the three-correlated factor solution loaded onto the same factors across the four samples. This shows that the scale measures three distinguishable, yet correlated constructs: 1) items related to how people feel in the morning, which we termed efficiency of dissipation of sleep pressure (recovery process) (items 1, 3, 4, 5, 7, 9, 13, and 19); 2) items related to how people feel before sleep, which we called sensitivity to build-up of sleep pressure (items 2, 10, and 12); and 3) peak time of cognitive arousal (item 11). Although the third factor was not regarded as consistent since only one item was common among all samples, it might represent subjective amplitude. These results suggested that the latent constructs of the MEQ reflect dissociable homeostatic processes in addition to a less consistent propensity for cognitive arousal at different times of the day. By analysing answers to MEQ items that compose these latent factors, it may be possible to extract further knowledge of factors that affect morningness-eveningness.

JKJ Diss, D Stewart, SP Fraser, JA Black, S Dib-Hajj, SG Waxman, SN Archer, MBA Djamgoz (1998)Expression of skeletal muscle-type voltage-gated Na+ channel in rat and human prostate cancer cell lines, In: FEBS LETTERS427(1)pp. 5-10 ELSEVIER SCIENCE BV
G Vandewalle, SN Archer, C Wuillaume, E Balteau, C Degueldre, A Luxen, DJ Dijk, P Maquet (2011)Effects of light on cognitive brain responses depend on circadian phase and sleep homeostasis., In: J Biol Rhythms26(3)pp. 249-259 Sage

Light is a powerful modulator of cognition through its long-term effects on circadian rhythmicity and direct effects on brain function as identified by neuroimaging. How the direct impact of light on brain function varies with wavelength of light, circadian phase, and sleep homeostasis, and how this differs between individuals, is a largely unexplored area. Using functional MRI, we compared the effects of 1 minute of low-intensity blue (473 nm) and green light (527 nm) exposures on brain responses to an auditory working memory task while varying circadian phase and status of the sleep homeostat. Data were collected in 27 subjects genotyped for the PER3 VNTR (12 PER3(5/5) and 15 PER3(4/4) ) in whom it was previously shown that the brain responses to this task, when conducted in darkness, depend on circadian phase, sleep homeostasis, and genotype. In the morning after sleep, blue light, relative to green light, increased brain responses primarily in the ventrolateral and dorsolateral prefrontal cortex and in the intraparietal sulcus, but only in PER3(4/4) individuals. By contrast, in the morning after sleep loss, blue light increased brain responses in a left thalamofrontoparietal circuit to a larger extent than green light, and only so in PER3(5/5) individuals. In the evening wake maintenance zone following a normal waking day, no differential effect of 1 minute of blue versus green light was observed in either genotype. Comparison of the current results with the findings observed in darkness indicates that light acts as an activating agent particularly under those circumstances in which and in those individuals in whom brain function is jeopardized by an adverse circadian phase and high homeostatic sleep pressure.

DJ Dijk, SN Archer (2010)PERIOD3, circadian phenotypes, and sleep homeostasis, In: SLEEP MED REV14(3)pp. 151-160 W B SAUNDERS CO LTD

Circadian rhythmicity and sleep homeostasis contribute to sleep phenotypes and sleep-wake disorders, some of the genetic determinants of which are emerging. Approximately 10% of the population are homozygous for the 5-repeat allele (PER3(5/5)) of a variable number tandem repeat polymorphism in the clock gene PERIOD3 (PER3). We review recent data on the effects of this polymorphism on sleep-wake regulation. PER3(5/5) are more likely to show morning preference, whereas homozygosity for the four-repeat allele (PER3(4/4)) associates with evening preferences. The association between sleep timing and the circadian rhythms of melatonin and PER3 RNA in leukocytes is stronger in PER3(5/5) than in PER3(4/4). EEG alpha activity in REM sleep, theta/alpha activity during wakefulness and slow wave activity in NREM sleep are elevated in PER3(5/5). PER3(5/5) show a greater cognitive decline, and a greater reduction in fMRI-assessed brain responses to an executive task, in response to total sleep deprivation. These effects are most pronounced during the late circadian night/early morning hours, i.e., approximately 0-4 h after the crest of the melatonin rhythm. We interpret the effects of the PER3 polymorphism within the context of a conceptual model in which higher homeostatic sleep pressure in PER3(5/5) through feedback onto the circadian pacemaker modulates the amplitude of diurnal variation in performance. These findings highlight the interrelatedness of circadian rhythmicity and sleep homeostasis. (C) 2009 Elsevier Ltd. All rights reserved.

DS Pereira, D. R. van der Veen, BSB Goncalves, S Tufik, M von Schantz, SN Archer, M Pedrazzoli (2014)The Effect of Different Photoperiods in Circadian Rhythms of Per3 Knockout Mice, In: BioMed Research International170795 Hindawi

The aim of this study was to analyse the circadian behavioural responses of mice carrying a functional knockout of the Per3 gene (𝑃𝑒𝑟3−/−) to different light : dark (L : D) cycles. Male adult wild-type (WT) and 𝑃𝑒𝑟3−/− mice were kept under 12-hour light : 12- hour dark conditions (12L : 12D) and then transferred to either a short or long photoperiod and subsequently released into total darkness. All mice were exposed to both conditions, and behavioural activity data were acquired through running wheel activity and analysed for circadian characteristics during these conditions. We observed that, during the transition from 12L : 12D to 16L : 8D, 𝑃𝑒𝑟3−/− mice take approximately one additional day to synchronise to the new L : D cycle compared to WT mice. Under these long photoperiod conditions, 𝑃𝑒𝑟3−/− mice were more active in the light phase. Our results suggest that 𝑃𝑒𝑟3−/− mice are less sensitive to light. The data presented here provides further evidence that Per3 is involved in the suppression of behavioural activity in direct response to light.

JC PARTRIDGE, SN ARCHER, JN LYTHGOE (1988)VISUAL PIGMENTS IN THE INDIVIDUAL RODS OF DEEP-SEA FISHES, In: JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY162(4)pp. 543-550 SPRINGER VERLAG
M von Schantz, A Jenkins, SN Archer (2006)Evolutionary history of the vertebrate Period genes, In: JOURNAL OF MOLECULAR EVOLUTION62(6)pp. 701-707 SPRINGER
J Sahnd, JC Partridge, S Archer, GW Potts, JN Lythgoe (2008)Spectral absorbance changes in the violet/blue sensitive cones of the juvenile pollack, Pollachius pollachius, In: Journal of Comparative Physiology A: sensory, neural, and behavioral physiology163(5)pp. 699-703 Springer

Using the technique of microspectrophotometry (MSP) we have found that the short wavelength sensitive cones in the retina of the pollack (Pollachius pollachius) shift in spectral absorption from a maximum (λ max) at about 420 nm in the violet to about 460 nm in the blue. This shift is not due to chromophore replacement, which substitutes rhodopsin for a porphyropsin, but is more likely to be due to a change in the opsin. The shift appears to be progressive rather than abrupt and coincides with a change in lifestyle of the fish.

K van der Heijden, MJ Blok, K Spee, SN Archer, MG Smits, LM Curfs, WB Gunning (2005)No evidence to support an association of PER3 clock gene polymorphism with ADHD-related idiopathic chronic sleep onset insomnia, In: BIOLOGICAL RHYTHM RESEARCH36(5)pp. 381-388 TAYLOR & FRANCIS LTD
C Wu, G Sui, SN Archer, P Sassone-Corsi, K Aitken, D Bagli, Y Chen (2014)Local receptors as novel regulators for peripheral clock expression, In: FASEB JOURNAL28(11)pp. 4610-4616 FEDERATION AMER SOC EXP BIOL
BJ Martynhak, AL Hogben, P Zanos, P Georgiou, R Andreatini, I Kitchen, SN Archer, M von Schantz, A Bailey, Daan R. van der Veen (2017)Transient anhedonia phenotype and altered circadian timing of behaviour during night-time dim light exposure in Per3-/- mice, but not wildtype mice, In: Scientific Reports740399 Nature Publishing Group

Industrialisation greatly increased human night-time exposure to artificial light, which in animal models is a known cause of depressive phenotypes. Whilst many of these phenotypes are ‘direct’ effects of light on affect, an ‘indirect’ pathway via altered sleep-wake timing has been suggested. We have previously shown that the Period3 gene, which forms part of the biological clock, is associated with altered sleep-wake patterns in response to light. Here, we show that both wild-type and Per3-/- mice showed elevated levels of circulating corticosterone and increased hippocampal Bdnf expression after 3 weeks of exposure to dim light at night, but only mice deficient for the PERIOD3 protein (Per3-/-) exhibited a transient anhedonia-like phenotype, observed as reduced sucrose preference, in weeks 2-3 of dim light at night, whereas WT mice did not. Per3-/- mice also exhibited a significantly smaller delay in behavioural timing than WT mice during weeks 1, 2 and 4 of dim light at night exposure. When treated with imipramine, neither Per3-/- nor WT mice exhibited an anhedonia-like phenotype, and neither genotypes exhibited a delay in behavioural timing in responses to dLAN. While the association between both Per3-/- phenotypes remains unclear, both are alleviated by imipramine treatment during dim night-time light.

Derk-Jan Dijk, Simon Archer (2009)Light, Sleep, and Circadian Rhythms: Together Again, In: PLOS BIOL7(6)e10001 PUBLIC LIBRARY SCIENCE
VS Staples, SN Archer, S Arber, DJ Skene (2009)Daily light exposure profiles in older non-resident extreme morning and evening types, In: Journal of Sleep Research18(4)pp. 466-471 Wiley-Blackwell Publishing

Differences in daily light exposure profiles have been reported, with younger M-types shown to spend more time in bright light, especially in the morning, compared with E-types. This study aimed to investigate how patterns of daily light exposure in older non-resident M-types and E-types compare. Sleep diaries were kept during actigraphic measurement of activity and light using the Actiwatch-L for 14 days in 12 M-types [eight females, mean ± standard deviation (SD) Horne–Östberg Morning–Eveningness Questionnaire (HÖ MEQ) score 75.2 ± 1.6] and 11 E-types (seven females, HÖ MEQ 41.5 ± 4.8), over 60 years old, living in their own homes. Light data were log-transformed, averaged over each hour, and group × time analysis of covariance (ancova) performed with age as a covariate. M-types had significantly earlier bed and wake time than E-types, but there was no significant difference in sleep duration, sleep efficiency or time spent in bed between groups. Daily exposure to light intensity greater than 1000 lux was compared between the two groups, with no significant difference in the duration of exposure to >1000 lux between M-types and E-types. Twenty-four-hour patterns of light exposure show that M-types were exposed to higher light intensity at 06:00 h than E-types. Conversely, E-types were exposed to higher light intensity between 22:00 and 23:00 h than M-types. These findings show that differences in daily light exposure patterns found previously in younger M-types and E-types are also found in older M-types and E-types, but at an earlier clock-time, confirming the tendency to advance with ageing.

N Santhi, HC Thorne, DR van der Veen, S Johnsen, SL Mills, V Hommes, LJ Schlangen, SN Archer, DJ Dijk (2011)The spectral composition of evening light and individual differences in the suppression of melatonin and delay of sleep in humans., In: J Pineal Res

The effect of light on circadian rhythms and sleep is mediated by a multi-component photoreceptive system of rods, cones and melanopsin-expressing intrinsically photosensitive retinal ganglion cells. The intensity and spectral sensitivity characteristics of this system are to be fully determined. Whether the intensity and spectral composition of light exposure at home in the evening is such that it delays circadian rhythms and sleep also remains to be established. We monitored light exposure at home during 6-8 wk and assessed light effects on sleep and circadian rhythms in the laboratory. Twenty-two women and men (23.1 ± 4.7 yr) participated in a six-way, cross-over design using polychromatic light conditions relevant to the light exposure at home, but with reduced, intermediate or enhanced efficacy with respect to the photopic and melanopsin systems. The evening rise of melatonin, sleepiness and EEG-assessed sleep onset varied significantly (P 

DL Robilliard, SN Archer, J Arendt, SW Lockley, LM Hack, J English, D Leger, MG Smits, A Williams, DJ Skene, M von Schantz (2002)The 3111 Clock gene polymorphism is not associated with sleep and circadian rhythmicity in phenotypically characterized human subjects, In: JOURNAL OF SLEEP RESEARCH11(4)pp. 305-312 BLACKWELL PUBLISHING LTD
S Archer, J Hirano, JKJ Diss, SP Fraser, MBA Djamgoz (1998)Expression and localization in the fish retina of a homologue of the Alzheimer's related PS1 gene, In: NEUROREPORT9(13)pp. 3115-3122 LIPPINCOTT WILLIAMS & WILKINS
MBA Djamgoz, MW Hankins, J Hirano, SN Archer (1997)Neurobiology of retinal dopamine in relation to degenerative states of the tissue, In: VISION RESEARCH37(24)pp. 3509-3529 PERGAMON-ELSEVIER SCIENCE LTD
Daan R. van der Veen, SN Archer (2010)Light-Dependent Behavioral Phenotypes in PER3-Deficient Mice, In: Journal of Biological Rhythms25(1)pp. 3-8 SAGE Publications

A functional knockout of Period3 in mice (mPer3— /—) results in a mildly altered circadian phenotype, and mPer3 shows a redundant role within the circadian clock. In this study, the authors reevaluated the Per3—/ — behavioral phenotype on a C57Bl/6J background and report altered responses to light. In constant light, free-running activity period was shorter than that of wild-type, whereas in constant darkness, no difference was observed between genotypes. The effect of light was parametric, and the difference in free-running period between genotypes increased under constant light with increasing light intensity. An attenuated response to light in Per3—/— mice was also demonstrated through reduced negative masking in activity in an ultradian protocol and a slower reentrainment to a shifted light-dark cycle when activity falls in the light period of the new light-dark cycle. Behavioral phase-shifts in response to a single delaying or advancing light pulse in the Per3—/— mouse were not compromised. This demonstrates that the mPer3— /— phenotype is characterized predominantly by altered sensitivity to light and not by the ability of the circadian system to respond to light. In addition to its redundant role within the molecular clock, these data suggest a new role for Per3 outside of the circadian clock and contributing to light input pathways.

JD Carpen, SN Archer, DJ Skene, M Smits, M von Schantz (2005)A single-nucleotide polymorphism in the 5 '-untranslated region of the hPER2 gene is associated with diurnal preference, In: JOURNAL OF SLEEP RESEARCH14(3)pp. 293-297 BLACKWELL PUBLISHING
HC Thorne, KH Jones, SP Peters, SN Archer, DJ Dijk (2009)Daily and Seasonal Variation in the Spectral Composition of Light Exposure in Humans, In: CHRONOBIOL INT26(5)pp. 854-866 Informa Healthcare

Light is considered the most potent synchronizer of the human circadian system and exerts many other non-image-forming effects, including those that affect brain function. These effects are mediated in part by intrinsically photosensitive retinal ganglion cells that express the photopigment melanopsin. The spectral sensitivity of melanopsin is greatest for blue light at approximately 480 nm. At present, there is little information on how the spectral composition of light to which people are exposed varies over the 24 h period and across seasons. Twenty-two subjects, aged 22±4 yrs (mean±SD) participated during the winter months (November–February), and 12 subjects aged 25±3 yrs participated during the summer months (April–August). Subjects wore Actiwatch-RGB monitors, as well as Actiwatch-L monitors, for seven consecutive days while living in England. These monitors measured activity and light exposure in the red, green, and blue spectral regions, in addition to broad-spectrum white light, with a 2 min resolution. Light exposure during the day was analyzed for the interval between 09:00 and 21:00 h. The time course of white-light exposure differed significantly between seasons (p = 0.0022), with light exposure increasing in the morning hours and declining in the afternoon hours, and with a more prominent decline in the winter. Overall light exposure was significantly higher in summer than winter (p = 0.0002). Seasonal differences in the relative contribution of blue-light exposure to overall light exposure were also observed (p = 0.0006), in particular during the evening hours. During the summer evenings (17:00–21:00 h), the relative contribution of blue light was significantly higher (p 

SL Chellappa, G Gaggioni, JQM Ly, S Papachilleos, C Borsu, A Brzozowski, M Rosanova, S Sarasso, A Luxen, Benita Middleton, Simon Archer, Derk-Jan Dijk, M Massimini, P Maquet, C Phillips, RJ Moran, G Vandewalle (2016)Circadian dynamics in measures of cortical excitation and inhibition balance, In: Scientific Reports633661 Nature Publishing Group

Several neuropsychiatric and neurological disorders have recently been characterized as dysfunctions arising from a ‘final common pathway’ of imbalanced excitation to inhibition within cortical networks. How the regulation of a cortical E/I ratio is affected by sleep and the circadian rhythm however, remains to be established. Here we addressed this issue through the analyses of TMS-evoked responses recorded over a 29h sleep deprivation protocol conducted in young and healthy volunteers. Spectral analyses of TMS-evoked responses in frontal cortex revealed non-linear changes in gamma band evoked oscillations, compatible with an influence of circadian timing on inhibitory interneuron activity. In silico inferences of cell-to-cell excitatory and inhibitory connectivity and GABA/Glutamate receptor time constant based on neural mass modeling within the Dynamic causal modeling framework, further suggested excitation/inhibition balance was under a strong circadian influence. These results indicate that circadian changes in EEG spectral properties, in measure of excitatory/inhibitory connectivity and in GABA/glutamate receptor function could support the maintenance of cognitive performance during a normal waking day, but also during overnight wakefulness. More generally, these findings demonstrate a slow daily regulation of cortical excitation/inhibition balance, which depends on circadian-timing and prior sleep-wake history.

G Vandewalle, SN Archer, C Wuillaume, E Balteau, C Degueldre, A Luxen, P Maquet, D-J Dijk (2009)Functional Magnetic Resonance Imaging-Assessed Brain Responses during an Executive Task Depend on Interaction of Sleep Homeostasis, Circadian Phase, and PER3 Genotype, In: JOURNAL OF NEUROSCIENCE29(25)pp. 7948-7956 SOC NEUROSCIENCE

Cognition is regulated across the 24 h sleep-wake cycle by circadian rhythmicity and sleep homeostasis through unknown brain mechanisms. We investigated these mechanisms in a functional magnetic resonance imaging study of executive function using a working memory 3-back task during a normal sleep-wake cycle and during sleep loss. The study population was stratified according to homozygosity for a variable-number (4 or 5) tandem-repeat polymorphism in the coding region of the clock gene PERIOD3. This polymorphism confers vulnerability to sleep loss and circadian misalignment through its effects on sleep homeostasis. In the less-vulnerable genotype, no changes were observed in brain responses during the normal-sleep wake cycle. During sleep loss, these individuals recruited supplemental anterior frontal, temporal and subcortical regions, while executive function was maintained. In contrast, in the vulnerable genotype, activation in a posterior prefrontal area was already reduced when comparing the evening to the morning during a normal sleep-wake cycle. Furthermore, in the morning after a night of sleep loss, widespread reductions in activation in prefrontal, temporal, parietal and occipital areas were observed in this genotype. These differences occurred in the absence of genotype-dependent differences in circadian phase. The data show that dynamic changes in brain responses to an executive task evolve across the sleep-wake and circadian cycles in a regionally specific manner that is determined by a polymorphism which affects sleep homeostasis. The findings support a model of individual differences in executive control, in which the allocation of prefrontal resources is constrained by sleep pressure and circadian phase.

C Meyer, V Muto, M Jaspar, C Kusse, E Lambot, SL Chellappa, C Degueldre, E Balteau, A Luxen, B Middleton, SN Archer, F Collette, D-J Dijk, C Phillips, P Maquet, G Vandewalle (2016)Seasonality in human cognitive brain responses, In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA113(11)pp. 3066-3071 NATL ACAD SCIENCES
S Hasan, Daan R. van der Veen, R Winsky-Sommerer, A Hogben, EE Laing, F Koentgen, DJ Dijk, SN Archer (2014)A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism, In: The FASEB Journal

In humans, a primate-specific variable-number tandem-repeat (VNTR) polymorphism (4 or 5 repeats 54 nt in length) in the circadian gene PER3 is associated with differences in sleep timing and homeostatic responses to sleep loss. We investigated the effects of this polymorphism on circadian rhythmicity and sleep homeostasis by introducing the polymorphism into mice and assessing circadian and sleep parameters at baseline and during and after 12 h of sleep deprivation (SD). Microarray analysis was used to measure hypothalamic and cortical gene expression. Circadian behavior and sleep were normal at baseline. The response to SD of 2 electrophysiological markers of sleep homeostasis, electroencephalography (EEG) θ power during wakefulness and δ power during sleep, were greater in the Per3(5/5) mice. During recovery, the Per3(5/5) mice fully compensated for the SD-induced deficit in δ power, but the Per3(4/4) and wild-type mice did not. Sleep homeostasis-related transcripts (e.g., Homer1, Ptgs2, and Kcna2) were differentially expressed between the humanized mice, but circadian clock genes were not. These data are in accordance with the hypothesis derived from human data that the PER3 VNTR polymorphism modifies the sleep homeostatic response without significantly influencing circadian parameters.-Hasan, S., van der Veen, D. R., Winsky-Sommerer, R., Hogben, A., Laing, E. E., Koentgen, F., Dijk, D.-J., Archer, S. N. A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism.

S Hasan, Daan R. van der Veen, R Winsky-Sommerer, DJ Dijk, SN Archer (2011)Altered sleep and behavioral activity phenotypes in PER3-deficient mice, In: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology301(6)pp. R1821-R1830 American Physiological Society

Sleep homeostasis and circadian rhythmicity interact to determine the timing of behavioral activity. Circadian clock genes contribute to circadian rhythmicity centrally and in the periphery, but some also have roles within sleep regulation. The clock gene Period3 (Per3) has a redundant function within the circadian system and is associated with sleep homeostasis in humans. This study investigated the role of PER3 in sleep/wake activity and sleep homeostasis in mice by recording wheel running activity under baseline conditions in wild-type (WT; n = 54) and in PER3-deficient (Per3(-/-); n = 53) mice, as well as EEG-assessed sleep before and after 6 hours of sleep deprivation in WT (n = 7) and Per3(-/-) (n = 8) mice. Whereas total activity and vigilance states did not differ between the genotypes, the temporal distribution of wheel running activity, vigilance states, and EEG delta activity was affected by genotype. In Per3(-/-) mice, running wheel activity was increased and REM sleep and NREM sleep were reduced in the middle of the dark phase, and delta activity was enhanced at the end of the dark phase. At the beginning of the baseline light period, there was less wakefulness and more REM and NREM in Per3(-/-) mice. Per3(-/-) mice spent less time in wakefulness and more time in NREM sleep in the light period immediately after sleep deprivation and REM sleep accumulated more slowly during the recovery dark phase. These data confirm a role for PER3 in sleep/wake timing and sleep homeostasis.

V Muto, M Jaspar, C Meyer, C Kussé, SL Chellappa, C Degueldre, E Balteau, A Shaffii-Le Bourdiec, A Luxen, B Middleton, SN Archer, C Phillips, F Collette, G Vandewalle, D Dijk, P Maquet (2016)Local Modulation of Human Brain Responses by Circadian Rhythmicity and Sleep Debt, In: Science353(6300)

Human performance results from an interaction between circadian rhythmicity and homeostatic sleep pressure. Whether and how this interaction is represented at the regional brain level is not established. We quantified changes in brain responses to a sustained-attention task during 13 functional magnetic resonance imaging (fMRI) sessions scheduled across the circadian cycle during 42h of wakefulness and following recovery sleep, in 33 healthy participants. Cortical responses showed significant circadian rhythmicity, the phase of which varied across brain regions. Cortical responses also significantly decreased with accrued sleep debt. Subcortical areas exhibited primarily a circadian modulation, which closely followed the melatonin profile. These findings expand our understanding of the mechanisms involved in maintaining cognition during the day and its deterioration during sleep deprivation and circadian misalignment.

NL Barclay, TC Eley, J Mill, CCY Wong, HMS Zavos, SN Archer, AM Gregory (2011)Sleep Quality and Diurnal Preference in a Sample of Young Adults: Associations With 5HTTLPR, PER3, and CLOCK 3111, In: AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS156B(6)pp. 681-690 WILEY-BLACKWELL
DR Van der Veen, SN Archer (2010)Light-dependent behavioral phenotypes in PER3-deficient mice (vol 25, pg 3, 2010), In: JOURNAL OF BIOLOGICAL RHYTHMS25(2)pp. 150-150 SAGE PUBLICATIONS INC
Emma Laing, Carla Moller-Levet, Norman Poh, Nayantara Santhi, Simon Archer, Derk-Jan Dijk (2017)Blood transcriptome based biomarkers for human circadian phase, In: Supplemental material for "Blood transcriptome based biomarkers for human circadian phase" eLife Sciences Publications Ltd

Diagnosis and treatment of circadian rhythm sleep-wake disorders requires assessment of circadian phase of the brain’s circadian pacemaker. The gold-standard univariate method is based on collection of a 24 h time series of plasma melatonin, a suprachiasmatic nucleus driven pineal hormone. We developed and validated a multivariate whole-blood mRNA based predictor of melatonin phase which requires few samples. Transcriptome data were collected under normal, sleep-deprivation and abnormal sleep-timing conditions to assess robustness of the predictor. Partial least square regression (PLSR), applied to the transcriptome, identified a set of 100 biomarkers primarily related to glucocorticoid signaling and immune function. Validation showed that PLSR-based predictors outperform published blood-derived circadian phase predictors. When given one sample as input, the R2 of predicted vs observed phase was 0.74, whereas for two samples taken 12 h apart, R2 was 0.90. This blood transcriptome based model enables assessment of circadian phase from a few samples.

EMMA ELIZABETH LAING, Carla Sofia Moller-Levet, Derk-Jan Dijk, Simon Archer Supplemental material for "Identifying and validating blood mRNA biomarkers for acute and chronic insufficient sleep in humans: a machine learning approach", In: Identifying and validating blood mRNA biomarkers for acute and chronic insufficient sleep in humans: a machine learning approach University of Surrey

Training and validation processed datasets used in the analyses can be found in the file called SleepDebt.zip which contains the following folders: TimeAwake, SleepIncreaseDecrease, ChronicSleepInsufficiency and AcuteSleepLoss. Each folder contains R object files [.RData] with training and validation datasets, prediction labels and R2 in each set and lambda used in the regression analyses. Instrument- or software-specific information needed to interpret the data: R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/. Further information about the methodology used can be found in the journal paper (Sleep, Volume 42, Issue 1, January 2019, zsy186).

EMMA ELIZABETH LAING, Carla Sofia Moller-Levet, Norman Poh, NAYANTARA SANTHI, Simon Archer, Derk-Jan Dijk (2017)Supplemental material for "Blood transcriptome based biomarkers for human circadian phase", In: Blood transcriptome based biomarkers for human circadian phase University of Surrey

Data sources and code used for the analyses are found in the file called PLSR_16.zip which contains the following files and folders: File_contents_description.txt (detailed description of the files included in the zip file), TRAINING_SAMPLES_MicroarrayInformation.csv (description of the microarray samples conforming the training set), Training_Processed_SingleDataset.csv (pre-processed, normalised and filtered (i.e. ready to use) microarray data matrix conforming the training set), Training_TwoSamples_SamplingTable.csv (table describing the pairing of samples 12 hrs apart within the training set), VALIDATION_SAMPLES_MicroarrayInformation.csv (description of the microarray samples conforming the validation set), Validation_Processed_SingleDataset.csv (pre-processed, normalised and filtered (i.e. ready to use) microarray data matrix conforming the validation set), Validation_TwoSamples_SamplingTable.csv (table describing the pairing of samples 12 hrs apart within the validation set), Code (folder with R scripts (.R) described in Code/RUNNING_CODE_README.txt file). Further information about the methodology used can be found in the journal paper https://doi.org/10.7554/eLife.20214.

Daan R. van der Veen, SN Archer (2012)Sleep-dependent learning, In: P Jarvis (eds.), The Routledge International Handbook of Learning(27)pp. 256-264 Routledge

The aim of this handbook is to present an overview of the work on learning, written by leading scholars from all these different perspectives and disciplines.

JF Schwarz, M Ingre, A Anund, C Fors, JG Karlsson, G Kecklund, DR Van der Veen, SN Archer, D Dijk, T Akerstedt (2012)Period3 VNTR polymorphism modifies sleepiness during real road driving, In: SLEEP35(Abstract Supplement)pp. A109-A109 Oxford University Press

Introduction: Individual differences in response to sleep loss have been described in various settings including driver sleepiness. A potential biological marker for this differential vulnerability is a PERIOD3 (PER3) Variable Number (4 or 5) Tandem Repeat polymorphism (rs57875989), for which homozygosity for the 5 repeat (PER35/5) has been associated with increased homeostatic sleep pressure and cognitive performance deficits in laboratory conditions. This is the first study so far experimentally investigating the effect of this polymorphism on sleepiness and performance outside the laboratory. Methods: 18 PER3 4/4 homozygotes and 10 PER3 5/5 homozygotes drove during day, evening and night for approximately 90 minutes on real roads. Subjective sleepiness was measured every 5th minute, physiological sleepiness (blink duration, delay of eyelid reopening) was measured continuously. Driving performance was averaged over the whole condition.Statistical analyses were conducted using multilevel mixed effects regression modelling. Results: Subjective sleepiness showed a steeper rise during evening and night conditions in PER3 5/5 individuals. The PER3 polymorphism was also associated with individual differences observed in one of the physiological sleepiness indicators (delay of eyelid reopening). While the standard deviation of lateral position and blink duration showed clear effects of condition and time on task, PER3 genotype was not significantly related to individual differences in these measures. Conclusion: The PER3 VNTR polymorphism contributed significantly to individual differences in subjective and physiological sleepiness during real road driving; yet observed individual differences were still pronounced.

D. R. van der Veen, D-J Dijk, SN Archer (2012)A role for PERIOD3 in sleep/wake rhythms: photic responses in humanised knock-in mice and gene expression correlates of PER3 expression, In: Journal of Sleep Research21(s1)pp. 333-333 Wiley

Objectives: Previously, we reported a light-dependant phenotype incircadian regulation in PER3 knockout (Per3-/-) mice. These mice also showed altered sleep architecture and elevated activity levels inthe second half of the dark period. In humans, a polymorphism inPER3 has been associated with diurnal preference, sleep homeo-stasis, and cognitive decline in response to sleep loss. We generated humanised knock-in (KI) mice expressing two variants of the human polymorphism and investigated activity patterns in response to different photoperiods. We also further investigated gene expression profiles of Per3-/- and KI mice during an ultradian light exposure paradigm. Methods: Male and female C57Bl/6 mice, expressing either the 4- or 5- repeat of the human variable number tandem repeat in PER3 (Per34/4 and Per35/5) were exposed to short (8 h), intermediate (12 h) and long (16 h) photoperiods, as well as constant light.Transitions between the conditions were mixed between animals,such that the response to a new photoperiod could be analysed,taking into account different light-histories. Behavioural activity was recorded as running wheel revolutions. In addition, we subjected Per3-/- and KI mice to an ultradian light-dark cycle (3.5 h L–3.5 h D) and analysed whole genome RNA expression at CT 16, in an ultradian light episode. Results: Significant differences between male and female activity were seen. Female mice showed more activity in the second half of the dark period, and overall 24-h activity levels were more than 1.5-fold higher in females. These differences were seen in all genotypes. In constant darkness, both male and female Per34/4 mice showed increased activity in the second half of the dark period, compared toWT and Per35/5 mice. The behavioural responses to photoperiods were diverse, with KI mice appearing to adjust more rapidly to a new photoperiod. Whole genome RNA expression in Per3-/-and KI mice was altered compared to WT mice, and similar pathways were affected in both Per3-/-and KI mice. Conclusion: Here we show behavioural data on a novel humanised mouse model of PER3. In mice, this polymorphism associates with altered activity, especially in the transition between photic conditions.We also observed a consistent difference between male and female activity. This emphasizes the need to not only use transgenic mice but also to include both sexes in animal models of human conditions.

SN Archer, EE Laing, CS Moller-Levet, Daan R. van der Veen, N Santhi, G Bucca, M von Schantz, AS Lazar, DJ Dijk, JCY Lo, A Slak, R Kabiljo, CP Smith (2014)Mistimed sleep disrupts the circadian regulation of the human transcriptome, In: JOURNAL OF SLEEP RESEARCH23pp. 15-15

Circadian organization of the mammalian transcriptome is achieved by rhythmic recruitment of key modifiers of chromatin structure and transcriptional and translational processes. These rhythmic processes, together with posttranslational modification, constitute circadian oscillators in the brain and peripheral tissues, which drive rhythms in physiology and behavior, including the sleep–wake cycle. In humans, sleep is normally timed to occur during the biological night, when body temperature is low and melatonin is synthesized. Desynchrony of sleep–wake timing and other circadian rhythms, such as occurs in shift work and jet lag, is associated with disruption of rhythmicity in physiology and endocrinology. However, to what extent mistimed sleep affects the molecular regulators of circadian rhythmicity remains to be established. Here, we show that mistimed sleep leads to a reduction of rhythmic transcripts in the human blood transcriptome from 6.4% at baseline to 1.0% during forced desynchrony of sleep and centrally driven circadian rhythms. Transcripts affected are key regulators of gene expression, including those associated with chromatin modification (methylases and acetylases), transcription (RNA polymerase II), translation (ribosomal proteins, initiation, and elongation factors), temperature-regulated transcription (cold inducible RNA-binding proteins), and core clock genes including CLOCK and ARNTL (BMAL1). We also estimated the separate contribution of sleep and circadian rhythmicity and found that the sleep–wake cycle coordinates the timing of transcription and translation in particular. The data show that mistimed sleep affects molecular processes at the core of circadian rhythm generation and imply that appropriate timing of sleep contributes significantly to the overall temporal organization of the human transcriptome.

G Vandewalle, SN Archer, C Wuillaume, E Balteau, C Degueldre, A Luxen, P Maquet, D Dijk (2008)A period 3 polymorphism predicts FMRI assessed brain responses following sleep loss, In: SLEEP31pp. A111-A111
S Hasan, R Winsky-Sommerer, D-J Dijk, SN Archer (2012)Sleep in transgenic mouse models for a polymorphism in the human PER3 gene, In: JOURNAL OF SLEEP RESEARCH21pp. 79-79
SN Archer, S Thompson, RJ Lucas, RG Foster, M von Schantz (2000)Analysis of differentially expressed genes in the wildtype and rd mouse retina by macroarray screening., In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE41(4)pp. S27-S27 ASSOC RESEARCH VISION OPHTHALMOLOGY INC
G Vandewalle, S Archer, C Wuillaume, E Balteau, C Degueldre, A Luxen, P Maquet, D Dijk (2008)Polymorphism in period3 predicts fMRI-assessed inter-individual differences in the effects of sleep deprivation, In: JOURNAL OF SLEEP RESEARCH17pp. 38-38
G Vandewalle, SN Archer, C Wuillaume, E Balteau, C Degueldre, A Luxen, D Dijk, P Maquet (2009)MODULATION OF FMRI ASSESSED BRAIN RESPONSES TO BLUE AND GREEN LIGHT BY SLEEP HOMFOSTASIS, CIRCADIAN PHASE AND PER3 POLYMORPHISM, In: SLEEP32pp. A1-A1
AU Viola, LM James, SN Archer, D Duk (2008)PER3 polymorphism affects cardiac autonomic control in healthy people, In: JOURNAL OF SLEEP RESEARCH17pp. 82-82
JC PARTRIDGE, SN ARCHER, J VANOOSTRUM (1992)SINGLE AND MULTIPLE VISUAL PIGMENTS IN DEEP-SEA FISHES, In: JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM72(1)pp. 113-130 CAMBRIDGE UNIV PRESS
SN Archer, J Hirano (1998)Rod opsin sequence in the John Dory: further evidence for the spectral tuning of rhodopsin, In: JOURNAL OF FISH BIOLOGY52(1)pp. 209-212 ACADEMIC PRESS LTD
G Gaggioni, JQM Ly, DC Wallant, V Muto, C Borsu, S Papachilleos, A Brzozowski, S Sarasso, M Rosanova, SN Archer, P Maquet, D-J Dijk, C Phillips, M Massimini, G Vandewalle, SL Chellappa (2014)Sleep slow-wave activity predicts changes in human cortical excitability during extended wakefulness, In: JOURNAL OF SLEEP RESEARCH23pp. 267-267
LM James, AU Viola, SN Archer, D Dijk (2008)Topography of the effects of a PER3 polymorphism on alpha activity in REM sleep under baseline and recovery conditions, In: JOURNAL OF SLEEP RESEARCH17pp. 1-1
J Groeger, J Lo, A Viola, M Von Schantz, S Archer, D Dijk (2007)PER3 polymorphism predictssusceptibility to sleep deprivation-induced impairment of early morning executive performance, In: SLEEP30pp. A54-A54
SL Chellappa, JQM Ly, G Gaggioni, S Papachilleos, C Borsu, A Brzozowski, SN Archer, M Rosanova, S Sarasso, D-J Dijk, P Maquet, M Massimini, C Phillips, RJ Moran, G Vandewalle (2014)Dynamics of human cortical ensembles are set by circadian system and sleep homeostasis, In: JOURNAL OF SLEEP RESEARCH23pp. 79-79
SN Archer, S Kitamura, N Santhi, D-J Dijk (2014)Effects of circadian typology and partial and total sleep deprivation on the human transcriptome, In: JOURNAL OF SLEEP RESEARCH23pp. 55-55
SN Archer (1999)Visual pigments and photoreception, In: Adaptive Mechanisms in the Ecology of Vision Springer Science & Business Media

This book represents a timely and much needed review of the wealth of research that has been carried out in the last twenty years.

JC Lo, JA Groeger, N Santhi, EL Arbon, AS Lazar, S Hasan, M Von Schantz, SN Archer, DJ Dijk (2012)Effects of circadian phase and prior partial sleep deprivation on executive functions during total sleep deprivation are modulated by PER3 polymorphism, In: JOURNAL OF SLEEP RESEARCH21pp. 41-41
JA Groeger, JC Lo, AU Viola, MV Schantz, SN Archer, D Dijk (2008)Period3 and the effects of sleep deprivation on executive function: the importance of circadian phase, In: JOURNAL OF SLEEP RESEARCH17pp. 38-38
N Santhi, AS Lazar, P Mccabe, SN Archer, JA Groeger, D-J Dijk (2014)Circadian regulation in cognition and subjective assessment of waking function in humans, In: JOURNAL OF SLEEP RESEARCH23pp. 48-48
M Von Schantz, SN Archer (2006)Genetic aspects of delayed sleep phase syndrome (DSPS), In: JOURNAL OF SLEEP RESEARCH15pp. 5-5
C Borsu, G Gaggioni, JQM Ly, S Papachilleos, A Brzozowski, M Rosanova, S Sarasso, SN Archer, D-J Dijk, C Phillips, P Maquet, M Massimini, SL Chellappa, G Vandewalle (2014)Cortical excitability dynamics during extended wakefulness set PVT performance, In: JOURNAL OF SLEEP RESEARCH23pp. 176-176
V Vaughan, S Archer, DJ Skene, S Arber (2008)Daily light exposure profiles in older extreme morning and evening types, In: JOURNAL OF SLEEP RESEARCH17pp. 73-73
P Ahuja, SN Archer, T van Veen, M von Schantz (2003)Neuroleukin/AMF is present around cones and in various spatially restricted cell types of the mouse retina, In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE44pp. U459-U459
M von Schantz, SN Archer, P Ahuja, T van Veen (2002)Identification of transcripts enriched in the inner retina by differential macroarray hybridization, In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE43pp. U321-U321
A Lazar, N Santhi, J Lo, A Slak, S Hasan, M Von Schantz, S Archer, D-J Dijk (2012)The circadian and homeostatic regulation of sleep spindle activity: effect of the PER3 VNTR polymorphism, In: JOURNAL OF SLEEP RESEARCH21pp. 82-82
S Archer (1999)Adaptive Mechanisms in the Ecology of Vision Springer Science & Business Media

This book represents a timely and much needed review of the wealth of research that has been carried out in the last twenty years.

C Meyer, M Jaspar, V Muto, C Kusse, SL Chellappa, C Degueldre, E Balteau, A Luxen, F Collette, B Middleton, C Phillips, SN Archer, D-J Dijk, G Vandewalle, P Maquet (2014)Seasonal variation in human executive brain responses, In: JOURNAL OF SLEEP RESEARCH23pp. 171-171
JA Groeger, CA Finnan, S Zoeller, KM Mcgregor, SN Archer (2012)Effects of genotype and chronotype on time use on work and rest days, In: JOURNAL OF SLEEP RESEARCH21pp. 333-334
JQM Ly, G Gaggioni, SL Chellappa, S Papachilleos, A Brzozowski, C Borsu, SN Archer, M Rosanova, S Sarrasso, D-J Dijk, C Phillips, P Maquet, M Massimini, G Vandewalle (2014)Human cortical excitability depends on time awake and circadian phase, In: JOURNAL OF SLEEP RESEARCH23pp. 9-10
SN Archer, RJ Lucas, S Thompson, RG Foster, M von Schantz (2002)Identification of a novel retinal kinase with promoter elements affected by circadian clock proteins, In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE43pp. U308-U308
M Jaspar, C Meyer, V Muto, A Shaffii-Le Bourdiec, C Kusse, G Vandewalle, F Collette, S Archer, D-J Dijk, P Maquet (2012)Influence of sleep homeostasis and circadian rhythm on executive discriminative ability during a constant routine, In: JOURNAL OF SLEEP RESEARCH21pp. 328-328
V Muto, M Jaspar, C Meyer, AS LeBourdiec, C Kussee, SL Chellappa, G Vandewalle, C Degueldre, A Luxen, F Collette, C Phillips, B Middleton, SN Archer, D-J Dijk, P Maquet (2014)Neural correlates of sustained attention under sleep deprivation during a constant routine: circadian and homeostatic interaction, In: JOURNAL OF SLEEP RESEARCH23pp. 61-61
EL Arbon, AS Lazar, JC Lo, A Slak, PJ Mccabe, J Boyle, SN Archer, D-J Dijk (2012)Individual differences in sleep revisited: stability across sleep restriction, extension and total sleep loss, In: JOURNAL OF SLEEP RESEARCH21pp. 207-208
SN Archer, AU Viola, V Kyriakopoulou, M von Schantz, DJ Dijk (2008)Inter-individual differences in habitual sleep timing and entrained phase of endogenous circadian rhythms of BMAL1, PER2 and PER3 mRNA in human leukocytes, In: SLEEP31(5)pp. 608-617 AMER ACAD SLEEP MEDICINE

Study Objectives: Individual sleep timing differs and is governed partly by circadian oscillators, which may be assessed by hormonal markers, or by clock gene expression. Clock gene expression oscillates in peripheral tissues, including leukocytes. The study objective was to determine whether the endogenous phase of these rhythms, assessed in the absence of the sleep-wake and light-dark cycle, correlates with habitual sleep-wake timing. Design: Observational, cross-sectional. Setting: Home environment and Clinical Research Center. Participants: 24 healthy subjects aged 25.0 ± 3.5 (SD) years. Measurements: Actigraphy and sleep diaries were used to characterize sleep timing. Circadian rhythm phase and amplitude of plasma melatonin, cortisol, and BMAL1, PER2, and PER3 expression were assessed during a constant routine. Results: Circadian oscillations were more robust for PER3 than for BMAL1 or PER2. Average peak timings were 6:05 for PER3, 8:06 for PER2, 15:06 for BMAL1, 4:20 for melatonin, and 10:49 for cortisol. Individual sleep-wake timing correlated with the phases of melatonin and cortisol. Individual PER3 rhythms correlated significantly with sleep-wake timing and the timing of melatonin and cortisol, but those of PER2 and BMAL1 did not reach significance. The correlation between sleep timing and PER3 expression was stronger in individuals homozygous for the variant of the PER3 polymorphism that is associated with morningness. Conclusions: Individual phase differences in PER3 expression during a constant routine correlate with sleep timing during entrainment. PER3 expression in leukocytes represents a useful molecular marker of the circadian processes governing sleep-wake timing.

A Shaffii-Le Bourdiec, V Muto, M Jaspar, C Kusse, A Foret, SN Archer, F Le Bourdiec, G Vandewalle, F Collette, DJ Dijk, P Maquet (2012)Difference in neural correlates of discrimination during sleep deprivation in PER3 homozygous, In: JOURNAL OF SLEEP RESEARCH21pp. 82-82
JA Groeger, JC Lo, N Santhi, EL Arbon, A Lazar, S Hasan, M Von Schantz, SN Archer, DJ Dijk (2012)'Trait-like' susceptibility to sleep loss varies with circadian phase and the task used to index vulnerable-resilient sleep-deprived performance, In: JOURNAL OF SLEEP RESEARCH21pp. 36-37
SN Archer, DL Robilliard, DJ Skene, M Smits, A Williams, J Arendt, M von Schantz (2003)A length polymorphism in the circadian clock gene Per3 is linked to delayed sleep phase syndrome and extreme diurnal preference, In: SLEEP26(4)pp. 413-415 AMER ACAD SLEEP MEDICINE
V Vaughan, R Meadows, SN Archer, DJ Skene, S Arber (2006)Diurnal preference in couples: negotiating sleep timing, In: JOURNAL OF SLEEP RESEARCH15pp. 95-95
JA Groeger, AU Viola, JCY Lo, M von Schantz, SN Archer, D-J Dijk (2008)Early morning executive functioning during sleep deprivation is compromised by a PERIOD3 polymorphism, In: SLEEP31(8)pp. 1159-1167 AMER ACAD SLEEP MEDICINE
DJ Skene, TL Sletten, K Ackermann, M Herljevic, KA Lederle, B Middleton, SN Archer, VL Revell (2009)LIGHT AND THE HUMAN CIRCADIAN TIMING SYSTEM: AGE-RELATED CHANGES, In: JOURNAL OF PHYSIOLOGICAL SCIENCES59pp. 37-37 SPRINGER TOKYO
SN Archer, JD Carpen, M Gibson, GH Lim, JD Johnston, DJ Skene, M von Schantz (2010)Polymorphism in the PER3 Promoter Associates with Diurnal Preference and Delayed Sleep Phase Disorder, In: SLEEP33(5)pp. 695-701 AMER ACAD SLEEP MEDICINE

Study Objectives: To screen the PER3 promoter for polymorphisms and investigate the phenotypic associations of these polymorphisms with diurnal preference, delayed sleep phase disorder/syndrome (DSPD/DSPS), and their effects on reporter gene expression. Design: Interspecific comparison was used to define the approximate extent of the PER3 promoter as the region between the transcriptional start site and nucleotide position −874. This region was screened in DNA pools using PCR and direct sequencing, which was also used to screen DNA from individual participants. The different promoter alleles were cloned into a luciferase expression vector and a deletion library created. Promoter activation was measured by chemiluminescence. Setting: N/A Patients or Participants: DNA samples were obtained from volunteers with defined diurnal preference (3 x 80, selected from a pool of 1,590), and DSPD patients (n = 23). Interventions: N/A Measurements and Results: We verified three single nucleotide polymorphisms (G −320T, C −319A, G −294A), and found a novel variable number tandem repeat (VNTR) polymorphism (−318 1/2 VNTR). The −320T and −319A alleles occurred more frequently in DSPD compared to morning (P = 0.042 for each) or evening types (P = 0.006 and 0.033). The allele combination TA2G was more prevalent in DSPD compared to morning (P = 0.033) or evening types (P = 0.002). Luciferase expression driven by the TA2G combination was greater than for the more common GC2A (P < 0.05) and the rarer TA1G (P < 0.001) combinations. Deletion reporter constructs identified two enhancer regions (−703 to −605, and −283 to −80). Conclusions: Polymorphisms in the PER3 promoter could affect its expression, leading to potential differences in the observed functions of PER3.

W Von Schantz, JD Carpen, M Gibson, G Lim, J Johnston, DJ Skene, SN Archer (2008)Analysis of the PER3 promoter and haplotypes that associate with diurnal preference and delayed sleep phase disorder, In: JOURNAL OF SLEEP RESEARCH17pp. 80-80
C Lintas, J Hirano, S Archer (1998)Genetic variation of the European eel (Anguilla anguilla), In: MOLECULAR MARINE BIOLOGY AND BIOTECHNOLOGY7(4)pp. 263-269 SPRINGER VERLAG
KH Jones, J Ellis, M Von Schantz, DJ Skene, D Dijk, SN Archer (2006)Age-related change in the association between a variable number tandem repeat polymorphism in the (PER3) gene and preferred timing of sleep and waking activities, In: JOURNAL OF SLEEP RESEARCH15pp. 97-98
AU Viola, SN Archer, JA Groeger, DJ Skene, M Von Schantz, DJ Dijk (2006)Polymorphism in the clock gene PER3 predicts sleep structure and EEG power spectra, In: JOURNAL OF SLEEP RESEARCH15pp. 53-53
S Archer (1998)Eels see in multicolour, In: BIOFUTUR(179)pp. 30-32 EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
M Jaspar, C Meyer, V Muto, A Shaffii-LeBourdiec, SL Chellappa, C Kussee, G Vandewalle, F Collette, B Middleton, S Archer, DJ Dijk, P Maquet (2014)Sleep loss changes executive brain responses in the wake maintenance zone, In: JOURNAL OF SLEEP RESEARCH23pp. 61-61
V Muto, C Meyer, M Jaspar, A Shaffii-Le Bourdiec, C Kusse, A Foret, G Vandewalle, F Collette, S Archer, D-J Dijk, P Maquet (2012)Influence of sleep homeostasis and circadian rhythm on waking EEG oscillations during a constant routine, In: JOURNAL OF SLEEP RESEARCH21pp. 327-328