Carina Dunlop

Dr Carina Dunlop


Senior Lecturer
+44 (0)1483 686524
06 AA 04

Biography

Areas of specialism

Biophysics; Mathematical Biology; Cancer modelling

University roles and responsibilities

  • Admissions Tutor

    Affiliations and memberships

    Society of Mathematical Biology
    Member (Developmental Biology subgroup)
    European Society for Mathematical and Theoretical Biology
    Member

    Research

    Research interests

    Research projects

    Supervision

    Postgraduate research supervision

    My teaching

    My publications

    Publications

    Erin A. Henslee, Carina Dunlop, Christine M. de Mel, Emily Carter, Rula Abdallat, Patrizia Camelliti, Fatima Labeed (2020)DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems, In: Scientific Reports1014603 Nature Research

    It is known that cells grown in 3D are more tolerant to drug treatment than those grown in dispersion but the mechanism for this is still not clear; cells grown in 3D have opportunities to develop inter-cell communication, but are also closely packed which may impede diffusion. In this study we examine methods for dielectrophoresis-based cell aggregation of both suspension and adherent cell lines and compare the effect of various drugs on cells grown in 3D and 2D. Comparing viability of pharmacological interventions on 3D cell clusters against both suspension cells and adherent cells grown in monolayer, as well as against a unicellular organism with no propensity for intracellular communication, we suggest that 3D aggregates of adherent cells, compared to suspension cells, show a substantially different drug response to cells grown in monolayer, which increases as the IC50 is approached. Further, a mathematical model of the system for each agent demonstrates that changes to drug response are due to inherent changes in the system of adherent cells from the 2D to 3D state. Finally, differences within electrophysiological membrane properties of the adherent cell type suggest this parameter plays an important role in the differences found in the 3D drug response.

    Euan Littlejohns, Carina Dunlop (2018)Mechanotransduction mechanisms in growing spherically structured tissues, In: New Journal of Physics20043041pp. 043041-1 IOP Publishing

    There is increasing experimental interest in mechanotransduction in multi-cellular tissues as opposed to single cells. This is driven by a growing awareness of the importance of physiologically relevant three-dimensional culture and of cell-cell and cell-gel interactions in directing growth and development. The paradigm biophysical technique for investigating tissue level mechanobiology in this context is to grow model tissues in artificial gels with well-defined mechanical properties. These studies often indicate that the sti↵ness of the encapsulating gel can significantly alter cellular behaviours. We demonstrate here potential mechanisms linking tissue growth with sti↵ness-mediated mechanotransduction. We show how tissue growth in gel systems generates points at which there is a significant qualitative change in the cellular stress and strain experienced. We show analytically how these potential switching points depend on the mechanical properties of the constraining gel and predict when they will occur. Significantly, we identify distinct mechanisms that act separately in each of the stress and strain fields at di↵erent times. These observations suggest growth as a potential physical mechanism coupling gel sti↵ness with cellular mechanotransduction in three-dimensional tissues. We additionally show that non-proliferating areas, in the case that the constraining gel is soft compared with the tissue, will expand and contract passively as a result of growth. Central compartment size is thus seen to not be a reliable indicator on its own for growth initiation or active behaviour.

    C Koke, T Kanesaki, J Grosshans, US Schwarz, CM Dunlop (2014)A computational model of nuclear self-organisation in syncytial embryos, In: Journal of Theoretical Biology359pp. 92-100

    Syncytial embryos develop through cycles of nuclear division and rearrangement within a common cytoplasm. A paradigm example is Drosophila melanogaster in which nuclei form an ordered array in the embryo surface over cell cycles 10-13. This ordering process is assumed to be essential for subsequent cellularisation. Using quantitative tissue analysis, it has previously been shown that the regrowth of actin and microtubule networks after nuclear division generates reordering forces that counteract its disordering effect (Kanesaki et al., 2011). We present here an individual-based computer simulation modelling the nuclear dynamics. In contrast to similar modelling approaches e.g. epithelial monolayers or tumour spheroids, we focus not on the spatial dependence, but rather on the time-dependence of the interaction laws. We show that appropriate phenomenological inter-nuclear force laws reproduce the experimentally observed dynamics provided that the cytoskeletal network regrows sufficiently quickly after mitosis. Then repulsive forces provided by the actin system are necessary and sufficient to regain the observed level of order in the system, after the strong disruption resulting from cytoskeletal network disassembly and spindle formation. We also observe little mixing of nuclei through cell cycles. Our study highlights the importance of the dynamics of cytoskeletal forces during this critical phase of syncytial development and emphasises the need for real-time experimental data at high temporal resolution. © 2014 Elsevier Ltd.

    US Schwarz, CM Dunlop (2012)Developmental Biology: A Growing Role for Computer Simulations, In: CURRENT BIOLOGY22(11)pp. R441-R443 CELL PRESS
    Carina Dunlop (2019)Differential cellular contractility as a mechanism for stiffness sensing, In: New Journal of Physics21063005pp. 1-9 IOP Publishing

    The ability of cells to sense and respond to the mechanical properties of their environments is fundamental to a range of cellular behaviours, with substrate stiffness increasingly being found to be a key signalling factor. Although active contractility of the cytoskeleton is clearly necessary for stiffness sensing in cells, the physical mechanisms connecting contractility with mechanosensing and molecular conformational change are not well understood. Here we present a contractility-driven mechanism for linking changes in substrate stiffness with internal conformational changes. Cellular contractility is often assumed to imply an associated compressive strain. We show, however, that where the contractility is non-uniform, localized areas of internal stretch can be generated as stiffer substrates are encountered. This suggests a physical mechanism for the stretch-activation of mechanotransductive molecules on stiffer substrates. Importantly, the areas of internal stretch occur deep within the cell and not near the cellular perimeter, which region is more traditionally associated with stiffness sensing through e.g. focal adhesions. This supports recent experimental results on whole-cell mechanically-driven mechanotransduction. Considering cellular shape we show that aspect ratio acts as an additional control parameter, so that the onset of positive strain moves to higher stiffness values in elliptical cells.

    Kate Hardy, Jocelyn M Mora, Carina Dunlop, Raffaella Carzaniga, Stephen Franks, Mark A Fenwick (2018)Nuclear exclusion of SMAD2/3 in granulosa cells is associated with cell proliferation and follicle activation in the mouse ovary., In: Journal of Cell Science131jcs218123 Company of Biologists

    Maintenance and activation of the limited supply of primordial follicles in the ovary are important determinants of reproductive lifespan. Currently, the molecular programme that maintains the primordial phenotype and the early events associated with follicle activation are not well defined. Here we have systematically analysed these events using microscopy and detailed image analysis. Using the immature mouse ovary as a model, we demonstrate that the onset of granulosa cell (GC) proliferation results in increased packing density on the oocyte surface and consequent GC cuboidalisation. These events precede oocyte growth and nuclear translocation of FOXO3a, a transcription factor important in follicle activation. Immunolabelling of the TGF signalling mediators and transcription factors, SMAD2/3, revealed a striking expression pattern specific to GCs of small follicles. SMAD2/3 was expressed in the nuclei of primordial GCs but was mostly excluded in early growing follicles. In activated follicles, GC nuclei lacking SMAD2/3 generally expressed Ki67. These findings suggest that the first phenotypic changes during follicle activation are observed in GCs, and that TGF signalling is fundamental for regulating GC arrest and the onset of proliferation.

    James W T Yates, Helen Byrne Byrne, Sonya C Chapman, Tao Chen, Lourdes Cucurull-Sanchez, Juan Delgado-SanMartin, Giovanni Di Veroli, Simon J Dovedi, Carina Dunlop, Rajesh Jena, Duncan Jodrell, Emma Martin, Francois Mercier, Antonio Ramos-Montoya, Herbert Struemper, Paolo Vicini (2020)Opportunities for quantitative translational modelling in Oncology, In: Clinical Pharmacology & Therapeutics American Society for Clinical Pharmacology and Therapeutics

    A two-day meeting was held by members of the UK Quantitative Systems Pharmacology Network (http://www.qsp-uk.net/) in November 2018 on the topic of Translational Challenges in Oncology. Participants from a wide range of backgrounds were invited to discuss current and emerging modelling applications in non-clinical and clinical drug development, and to identify areas for improvement. This resulting perspective explores opportunities for impactful quantitative pharmacology approaches. Four key themes arose from the presentations and discussions that were held, leading to the following recommendations: - Evaluate the predictivity and reproducibility of animal cancer models through pre-competitive collaboration - Apply mechanism of action (MoA) based mechanistic model derived from nonclinical data to clinical trial data - Apply MoA reflective models across trial data sets to more robustly quantify the natural history of disease and response to differing interventions - Quantify more robustly the dose and concentration dependence of adverse events through mathematical modelling techniques and modified trial design

    James W.T. Yates, Helen Byrne, Sonya C. Chapman, Tao Chen, Lourdes Cucurull‐Sanchez, Juan Delgado‐SanMartin, Giovanni Di Veroli, Simon J. Dovedi, Carina Dunlop, Rajesh Jena, Duncan Jodrell, Emma Martin, Francois Mercier, Antonio Ramos‐Montoya, Herbert Struemper, Paolo Vicini (2020)Opportunities for Quantitative Translational Modeling in Oncology, In: Clinical Pharmacology & Therapeutics108(3)pp. 447-457 Wiley

    A 2‐day meeting was held by members of the UK Quantitative Systems Pharmacology Network (http://www.qsp‐uk.net/) in November 2018 on the topic of Translational Challenges in Oncology. Participants from a wide range of backgrounds were invited to discuss current and emerging modeling applications in nonclinical and clinical drug development, and to identify areas for improvement. This resulting perspective explores opportunities for impactful quantitative pharmacology approaches. Four key themes arose from the presentations and discussions that were held, leading to the following recommendations: • Evaluate the predictivity and reproducibility of animal cancer models through precompetitive collaboration. • Apply mechanism of action (MoA) based mechanistic models derived from nonclinical data to clinical trial data. • Apply MoA reflective models across trial data sets to more robustly quantify the natural history of disease and response to differing interventions. • Quantify more robustly the dose and concentration dependence of adverse events through mathematical modelling techniques and modified trial design.