Basidiomycete fungi are a rich source of natural products with a diverse array of potentially
exploitable bioactivities. Two dimeric sesquiterpenes, bovistol B (1) and D (2), and one
monomeric sesquiterpene, strossmayerin (7), were isolated from the culture filtrate of the
basidiomycete fungus Coprinopsis strossmayeri. The structures were determined through a
combination of MS and 1D/2D NMR spectroscopic techniques. Likely monomeric precursors,
identified on the basis of HRMS analysis, allow a plausible biosynthetic pathway to be
proposed for the biosynthesis of 1 and 2, involving the dimerisation of the monomer through
a hetero-Diels-Alder mechanism. A gene cluster, including a putative sesquiterpene 1?11
cyclase, was identified through phylogenetic and RNA-seq analysis, and is proposed to be
responsible for the biosynthesis of 1 and 2.
Fungi are often inconspicuous in nature and this means it is all too easy to overlook their importance. Often referred to as the?Forgotten Kingdom?, fungi arekey components of life on this planet. The phylumBasidiomycota, considered to contain the most complex and evolutionarily advanced members of this Kingdom,includes some of the most iconic fungal species such as the gilled mushrooms, puffballs and bracket fungi. Basidiomycetes inhabit a wide range of ecological niches,carrying out vital ecosystem roles, particularly in carbon cycling and as symbiotic partners with a range of other organisms. Specifically in the context of human use, thebasidiomycetes are a highly valuable food source and are increasingly medicinally important. In this review, seven main categories, or?roles?, for basidiomycetes havebeen suggested by the authors: as model species, edible species, toxic species, medicinal basidiomycetes, symbionts, decomposers and pathogens, and two specieshave been chosen as representatives of each category. Although this is in no way an exhaustive discussion of the importance of basidiomycetes, this review aims to givea broad overview of the importance of these organisms, exploring the various ways they can be exploited to the benefit of human society.
The fungus Lecanicillium fungicolacauses dry bubble disease in the white button mushroom Agaricus bisporus. Control strategies are limited, as boththe host and pathogen are fungi, and there is limited understanding of the interac-tions in this pathosystem. Here, we present the genome sequence of Lecanicillium fungicola strain 150-1.
Coprinopsis strossmayeri is a coprinoid mushroom favoring the habitat of herbivore dung. As a result of this highly competitive environment, C. strossmayeri
is anticipated to produce a wide array of antimicrobial secondary metabolites (SMs)
of potential pharmaceutical importance. Here, we present the draft genome sequence of C. strossmayeri.
We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.